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Enhancement of Rubisco content of rice leaves by an overexpression of rbcS

Research Project

Project/Area Number 19780047
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Plant nutrition/Soil science
Research InstitutionTohoku University

Principal Investigator

SUZUKI Yuji  Tohoku University, 大学院・農学研究科, 助教 (80374974)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,740,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥2,700,000 (Direct Cost: ¥2,700,000)
KeywordsRubisco / RBCS / 過剰発現 / RBCL / 光合成 / 葉の老化 / イネ / mRNA量 / 老化 / 個体生育
Research Abstract

イネ葉において,光合成の鍵酵素であるRubiscoの量をRubiscoの小サブユニットの遺伝子であるrbcSを過剰発現させることで増強することに成功した.しかし,光合成速度は葉の一生を通じて改善されなかった.その要因として,成熟葉ではRubiscoの酵素活性が低下していたこと,また,老化葉ではRubiscoの量が増強されておらず野生型と同程度にまで低下していたことが挙げられた.

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (26 results)

All 2009 2008 2007 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (13 results) Remarks (1 results)

  • [Journal Article] Rubisco content and photosynthesis of leaves at different positions in transgenic rice with an overexpression of RBCS2009

    • Author(s)
      Y. SUZUKI, T. MIYAMOTO, R. YOSHIZAWA, T. MAE, A. MAKINO
    • Journal Title

      Plant, Cell and Environment 32

      Pages: 417-427

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparative transcriptomic characterization of aluminum, sodium chloride, cadmium and copper rhizotoxicities in Arabidopsis thaliana2009

    • Author(s)
      C.R. Zhao, T. Ikka, Y. Sawaki, Y. Kobayashi, Y. Suzuki, T. Hibino, S. Sato, N. Sakurai, D. Shibata, H. Koyama
    • Journal Title

      BMC Plant Biology 9

      Pages: 32-32

    • NAID

      120002337685

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rubisco content and photosynthesis of leaves at different positions in transgenic rice with an overexpression of RBCS2009

    • Author(s)
      Y. Suzuki
    • Journal Title

      Plant, Cell and Environment 32

      Pages: 417-427

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparative transcriptomic characterization of aluminum, sodium chloride, cadmium and copper rhizotoxicities in Arabidopsis thaliana2009

    • Author(s)
      Cheng-Ri Zhao
    • Journal Title

      BMC Plant Biology 9(in press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased Rubisco Content in Transgenic Rice Transformed with "Sense" rbcS Gene2008

    • Author(s)
      Y. SUZUKI, M. OHKUBO, H. HATAKEYAMA, K. OHASHI, R. YOSHIZAWA, S. KOJIMA, T. HAYAKAWA, T. YAMAYA, T. MAE, A. MAKINO
    • Journal Title

      Energy from the Sun: 14th International Congress on Photosynthesis(J.F. Allen, E. Gantt, J.H. Golbeck, and B. Osmond (eds.), Photosynthesis)(Springer)

    • NAID

      10027001603

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] RNA extraction from various recalcitrant plant tissues with a cethyltrimethylammonium bromide- containing buffer followed by acid guanidium-thiocyanate-phenol-chloroform treatment2008

    • Author(s)
      Y. SUZUKI, T. MAE, A. MAKINO
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry 72

      Pages: 1951-1953

    • NAID

      10027530447

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Changes in the synthesis in the rice leaves in relation to senescence and N influx2008

    • Author(s)
      K. IMAI, Y. SUZUKI, T. MAE, A. MAKINO
    • Journal Title

      Annals of Botany 101

      Pages: 135-144

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Increased Rubisco Content in Transgenic Rice Transformed with "Sense" rbcS Gene2008

    • Author(s)
      Y. Suzuki
    • Journal Title

      Photosynthesis. Energy form the Sun

      Pages: 885-888

    • NAID

      10027001603

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Changes in the Synthesis of Rubisco in Rice Leaves in Relation to Senescence and N Influx2008

    • Author(s)
      K. Imai
    • Journal Title

      Annals of Botany 101

      Pages: 135-144

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RNA Extraction from Various Recalcitrant Plant Tissues with a Cethyltrimethylammonium Bromide-Containing Buffer Followed by Acid Guanidium Thiocyanate-Phenol-Chloroform Treatment2008

    • Author(s)
      Y. Suzuki
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry 72(In press)

    • NAID

      10027530447

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased Rubisco Content in Transgenic Rice Transformed with "Sense" rbcS Gene2007

    • Author(s)
      Y. SUZUKI, M. OHKUBO, H. HATAKEYAMA, K. OHASHI, R. YOSHIZAWA, S. KOJIMA, T. HAYAKAWA, T. YAMAYA, T. MAE, A. MAKINO
    • Journal Title

      Plant and Cell Physiology 48

      Pages: 1472-1483

    • NAID

      10027001603

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Increased Rubisco Content in Transgenic Rice Transformed with the'Sense'rbcS Gene2007

    • Author(s)
      Y. Suzuki
    • Journal Title

      Plant and Cell Physiology 48

      Pages: 626-637

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Changes in Rubisco content during leaf development in Eucalyptus globulus2009

    • Author(s)
      Y. Suzuki
    • Organizer
      NISSAN SCIENCE FOUNDATION Woody Plants Biotechnology Symposium
    • Place of Presentation
      東京, 千代田区
    • Year and Date
      2009-02-26
    • Related Report
      2008 Final Research Report
  • [Presentation] Changes in Rubisco content during leaf development in Eucalyptus globulus2009

    • Author(s)
      Y. Suzuki
    • Organizer
      NISSAN SCIENCE FOUNDATION Woody Plants Biotechnology Symposium
    • Place of Presentation
      東京、千代田区
    • Year and Date
      2009-02-26
    • Related Report
      2008 Annual Research Report
  • [Presentation] RBCS遺伝子を過剰発現した形質転換体イネのRubisco量,光合成速度および個体生育2009

    • Author(s)
      鈴木 雄二
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      名古屋, 千種区
    • Related Report
      2008 Final Research Report
  • [Presentation] RBCS遺伝子を過剰発現した形質転換体イネのRubisco量, 光合成速度および個体生育2009

    • Author(s)
      鈴木雄二
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      名古屋、千種区
    • Related Report
      2008 Annual Research Report
  • [Presentation] RBCS遺伝子を過剰発現したイネの葉の一生におけるRubisco量および光合成特性の変化2008

    • Author(s)
      鈴木 雄二
    • Organizer
      日本土壌肥料学会年会
    • Place of Presentation
      名古屋, 瑞穂区
    • Related Report
      2008 Final Research Report
  • [Presentation] CTABを含む緩衝液とAGPC処理を用いた厄介な植物組織からのRNA抽出法2008

    • Author(s)
      鈴木 雄二
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      札幌
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] RBCS遺伝子を過剰発現したイネの葉の一生におけるRubiSCQ量および光合成特性の変化2008

    • Author(s)
      鈴木雄二
    • Organizer
      日本土壌肥料学会年会
    • Place of Presentation
      名古屋、瑞穂区
    • Related Report
      2008 Annual Research Report
  • [Presentation] イネにおけるrbcS gene familyの部位特異的発現特性2007

    • Author(s)
      鈴木 雄二
    • Organizer
      日本土壌肥料学会年会
    • Place of Presentation
      東京, 世田谷区
    • Related Report
      2008 Final Research Report
  • [Presentation] Increased Rubisco content in transgenic rice transformed with "sense" rbcS gene2007

    • Author(s)
      Y. Suzuki
    • Organizer
      14th International Congress on Photosynthesis
    • Place of Presentation
      Glasgow, 英国
    • Related Report
      2008 Final Research Report
  • [Presentation] Increased Rubisco content in transgenic rice transformed with "sense" rbcS gene2007

    • Author(s)
      Y. Suzuki
    • Organizer
      Research Frontiers with Rubisco, the "Elixir of Life" in the Biosphere
    • Place of Presentation
      Harpenden, 英国
    • Related Report
      2008 Final Research Report
  • [Presentation] Increased Rubisco content in transgenic rice transformed with "sense" rbcS gene2007

    • Author(s)
      Y. Suzuki
    • Organizer
      Research Frontiers with Rubisco, the "Elixir of Life" in the Biosphere
    • Place of Presentation
      Harpenden、英国
    • Related Report
      2007 Annual Research Report
  • [Presentation] Increased Rubisco content in transgenic rice transformed with "sense" rbcS gene2007

    • Author(s)
      Y. Suzuki
    • Organizer
      14th International Congress on Photosynthesis
    • Place of Presentation
      Glasgow、英国
    • Related Report
      2007 Annual Research Report
  • [Presentation] イネにおけるrbcS gene familyの部位特異的発現特性2007

    • Author(s)
      鈴木 雄二
    • Organizer
      日本土壌肥料学会年会
    • Place of Presentation
      東京、世田谷
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.agri.tohoku.ac.jp/syokuei/index-j.html

    • Related Report
      2008 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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