Gene regulation system under Fe-deficiency in rice

• Project/Area Number: 19780048
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant nutrition/Soil science
• Research Institution: The University of Tokyo
• Principal Investigator: NAKANISHI Hiromi The University of Tokyo, 大学院農学生命科学研究科, 助教 (80282698)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,760,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: 植物成長 / 生理 / 鉄 / ムギネ酸類 / 遺伝子発現 / 転写因子 / シスエレメント

Research Abstract

イネ科植物の鉄欠乏に応答して遺伝子の発現を引き起こすシスエレメントIDE1とIDE2に結合する転写因子の検索とそれらの解析を行った。IDEF1とIDEF2、二つの転写因子を単離・同定することに成功した。また、鉄獲得に重要な転写因子IRO2も単離した。これらの転写因子を用いて鉄欠乏耐性の組換え植物を作成した。DEF1、IDEF2、OsIRO2が鉄欠乏応答に関与する多段階の遺伝子制御ネットワークを担うによって鉄欠乏応答性と耐性を制御することを明らかにした。

Research Products

• [Journal Article] A novel NAC transcription factor IDEF2 that recognizes the iron deficiency-responsive element 2 regulates the genes involved in iron homeostasis in plants (2008)
  • Author(s): Ogo Y, Kobayashi T, Itai RN, Nakanishi H, Kakei Y, Takahashi M, Toki S, Mori S, Nishizawa NK
  • Journal Title: J Biol Chem 283 Pages: 13407-13417
  • NAID: 130006994069
• [Journal Article] A novel NAC transcription factor IDEF2 that recognizes the iron deficiency-respo nsive element 2 regulates the genes involved in iron homeostasis in plants (2008)
  • Author(s): Ogo Y, Kobayashi T, Itai RN, Naka nishi H, Kakei Y, Takahashi M, Tok i S, Mori S, Nishizawa NK
  • Journal Title: J Biol Chem 283 Pages: 13407-13417
  • Peer Reviewed
• [Journal Article] The transcription factor IDEF1 regulates the response to and tolerance of iron deficiency in plants (2007)
  • Author(s): Kobayashi T, Ogo Y, Nakanishi Itai R, Nakanishi H, Takahashi M, Mori S, Nishizawa NK
  • Journal Title: Proc Natl Acad Sci USA 104 Pages: 19150-19155
  • Peer Reviewed
• [Journal Article] The rice bHLH protein OsIRO2 is an essential regulator of the genes involved in Fe uptake under Fe-deficient conditions (2007)
  • Author(s): Ogo Y, Nakanishi Itai R, Nakanishi H, Kobayashi T, Takahashi M, Mori S, Nishizawa NK
  • Journal Title: Plant J 51 Pages: 366-377
  • NAID: 130006990523
  • Peer Reviewed
• [Journal Article] The transcription factor IDEF1 regulates the response to and tolerance of iron deficiency in plants. (2007)
  • Author(s): Kobayashi T, Ogo Y, Nakanishi Itai R, Nakanishi H, Takahashi M, Mori S, Nishizawa NK
  • Journal Title: Proceedings of the National Academy of Sciences of the USA 104 Pages: 19150-19155
  • Peer Reviewed
• [Journal Article] The rice bHLH protein OsIRO2 is an essential regulator of the genes involved in Fe uptake under Fe-deficient conditions (2007)
  • Author(s): Ogo Y, Nakanishi Itai R, Nakanishi H, Kobayashi T, Takahashi M, Mori S, Nishizawa NK
  • Journal Title: The Plant Journal 51 Pages: 366-377
  • NAID: 130006990523
  • Peer Reviewed
• [Presentation] 鉄欠乏応答性シスエレメントIDE2に結合する新規NAC 型転写因子IDEF2の単離と解析 (2009)
  • Author(s): 小郷裕子、小林高範、板井玲子、中西啓仁、筧雄介、高橋美智子、土岐精一、森敏、西澤直子
  • Organizer: 日本植物生理学会
  • Place of Presentation: 名古屋
• [Presentation] 鉄欠乏応答性シスエレメント結合性転写因子IDEF1は鉄欠乏の初期応答に必須である (2009)
  • Author(s): 小林高範、板井玲子、小郷裕子、筧雄介、中西啓仁、高橋美智子、西澤直子
  • Organizer: 日本植物生理学会
  • Place of Presentation: 名古屋
• [Presentation] 植物の鉄欠乏応答と耐性を制御する新規転写因子IDEF1 (2008)
  • Author(s): 小林高範、小郷裕子、板井玲子、中西啓仁、高橋美智子、森敏、西澤直子
  • Organizer: 日本植物生理学会
  • Place of Presentation: 札幌
• [Presentation] 鉄欠乏誘導性転写因子OsIRO2 過剰発現は石灰質アルカリ土壌においてイネの鉄吸収および移行を向上させる (2008)
  • Author(s): 小郷裕子、板井玲子、中西啓仁、小林高範、高橋美智子、森敏、西澤直子
  • Organizer: 日本植物生理学会
  • Place of Presentation: 札幌
• [Presentation] 鉄の吸収と体内移行に関わる遺伝子の発現機構 (2008)
  • Author(s): 中西啓仁、小林高範、小郷裕子、板井玲子、西澤直子
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 愛知
• [Presentation] IDE1結合性転写因子IDEF1は鉄欠乏応答と耐性を制御する (2008)
  • Author(s): 小林高範、小郷裕子、板井玲子、中西啓仁、高橋美智子、西澤直子
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 愛知
• [Presentation] 鉄欠乏応答性シスエレメントIDE2 に結合する新規NAC型転写因子IDEF2の単離と解析 (2008)
  • Author(s): 小郷裕子、小林高範、板井玲子、中西啓仁、筧雄介、高橋美智子、土岐精一、森敏、西澤直子
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 愛知
• [Presentation] The novel transcription factor IDEF1(IDE-binding factor 1) regulates the iron deficiency response and tolerance in plants (2008)
  • Author(s): Kobayashi T, Ogo Y, Itai RN, Nakanishi H, Takahashi M, Mori S, NishizawaNK
  • Organizer: XIV International Symposium on Iron Nutrition and Interactions in Plants
  • Place of Presentation: Beijing
• [Presentation] Gene profiling in the early iron-deficiency stress in rice roots (2008)
  • Author(s): Itai RN, Ogo Y, Kobayashi T, Takahashi M, Nakanishi H, Mori S, Nishizawa NK
  • Organizer: XIV International Symposium on Iron Nutrition and Interactions in Plants
  • Place of Presentation: Beijing
• [Presentation] Overexpression of the transcription factor OsIRO2 improved both uptake and translocation of iron in rice under low iron availability conditions (2008)
  • Author(s): Ogo Y, Itai RN, Nakanishi H, Kobayashi T, Takahashi M, Mori S, Nishizawa NK
  • Organizer: XIV International Symposium on Iron Nutrition and Interactions in Plants
  • Place of Presentation: Beijing
• [Presentation] A novel NAC transcription factor IDEF2 that recognizes the iron deficiency-responsive element 2 regulates the genes involved in iron homeostasis in plants (2008)
  • Author(s): Ogo Y, Kobayashi T, Itai RN, Nakanishi H, Kakei Y, Takahashi M, Toki S, Mori S, Nishizawa NK
  • Organizer: XIV International Symposium on Iron Nutrition and Interactions in Plants
  • Place of Presentation: Beijing
• [Presentation] Regulation of iron-acquisition-related rice genes by novel iron-deficiency-responsive cisacting elements, IDE1 and IDE2 (2007)
  • Author(s): Kobayashi T, Nakayama Y, Yoshihara T, Suzuki M, Inoue H, Itai RN, Ogo Y, Takahashi M, Nakanishi H, Mori S, Nishizawa NK
  • Organizer: 第5回イネ国際機能ゲノムミックスシンポ
  • Place of Presentation: つくば
• [Presentation] ムギネ酸類合成に関わる遺伝子群を制御する転写因子OsIRO2過剰発現イネは鉄欠乏耐性を示す (2007)
  • Author(s): 小郷裕子、板井玲子、中西啓仁、小林高範、高橋美智子、森敏、西澤直子
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 東京
• [Presentation] Regulation of iron-acquisition-related rice genes by novel iron-deficiency-responsive cis-acting elements, IDE1 and IDE2 (2007)
  • Author(s): Kobayashi T, Nakayama Y, Yoshihara T, Suzuki M, Inoue H, Itai RN, Ogo Y, Takahashi M, Nakanishi H, Mori S, Nishizawa NK
  • Organizer: 第5回イネ国際機能ゲノムミックスシンポ(5th ISRFG: The 5th International Symposium of Rice Functional Genomics)
  • Place of Presentation: つくば