Molecular basis of one-carbon metabolism of microorganisms and the regulatory mechanism of the gene expression

• Project/Area Number: 19780058
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: Kyoto University
• Principal Investigator: YURIMOTO Hiroya Kyoto University, 大学院・農学研究科, 准教授 (00283648)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,760,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: メタノール / ホルムアルデヒド / Candida boidinii / プロモーター / リブロースモノリン酸経路 / Mycobacterium gastri / HPS / PHI

Research Abstract

酵母メタノール誘導性遺伝子発現に関わる転写因子を新たに取得し、Trm1 がメタノール特異的誘導、Trm2 がグルコース脱抑制に関わる転写因子として機能していることを明らかにした。枯草菌のホルムアルデヒド応答性遺伝子発現制御機構を利用し、ホルムアルデヒドを検出できるセンサー細胞を開発した。メタノール資化性細菌のホルムアルデヒド固定経路の酵素(HPS, PHI)を人工的に融合した二機能性酵素を創製し、ホルムアルデヒド資化性や耐性を異種生物に付与することに成功した。

Research Products

• [Journal Article] Activation of the oxidative stress regulator PpYap1 through conserved cysteine residues during methanol metabolism in the yeast Pichia pastoris (2009)
  • Author(s): Yano, T., H. Yurimoto, Y. Sakai
  • Journal Title: Biosci. Biotechnol. Biochem. (in press)
  • NAID: 10027542721
  • Peer Reviewed
• [Journal Article] Methanolinducible gene expression and heterologous protein production in the methylotrophic yeast Candida boidinii (2009)
  • Author(s): Yurimoto H., Y. Sakai
  • Journal Title: Biotechnol. Appl. Biochem. 53 Pages: 85-92
  • Peer Reviewed
• [Journal Article] Molecular basis of methanol-inducible gene expression and its application in the methylotrophic yeast Candida boidinii (2009)
  • Author(s): Yurimoto, H.
  • Journal Title: Biosci. Biotechnol. Biochem. 73 Pages: 793-800
  • NAID: 10027539388
  • Peer Reviewed
• [Journal Article] The Yap-1 regulated glutathione redox system curtails the accumulation of formaldehyde and reactive oxygen species in methanol metabolism of Pichia pastoris (2009)
  • Author(s): Yano, T., E. Takigami, H. Yurimoto, Y. Sakai.
  • Journal Title: Eukaryot. Cell 8 Pages: 540-549
  • Peer Reviewed
• [Journal Article] Overexpression and purification of rat peroxisomal membrane protein 22, PMP22, in Pichia pastoris (2009)
  • Author(s): Egawa, K., H. Shibata, S. Yamashita, H. Yurimoto, Y. Sakai, H. Kato
  • Journal Title: Protein Expr. Purif. 64 Pages: 47-54
  • Peer Reviewed
• [Journal Article] Overexpression and purification of rat peroxismal membrane protein 22, PMP22, in Pichia pastoris. (2009)
  • Author(s): Egawa, K., et.al.
  • Journal Title: Protein Expr. Purif. 64 Pages: 47-54
  • Peer Reviewed
• [Journal Article] The Yap1-regulated. glutathione redox system curtails the accumulation of formaldehyde and reactive oxygen species in methanol metabolism of Pichia nastoris (2009)
  • Author(s): Yano, T., et.al.
  • Journal Title: Eukaryot. Cell 8 Pages: 540-549
  • Peer Reviewed
• [Journal Article] Activation of the oxidative stress regplator PpYapl through conserved cysteine residues during methanol metabolism in. the Pichia pastoris. (2009)
  • Author(s): Yano, T., et.al.
  • Journal Title: Biosci. Biotechnol. Biochem.
  • Peer Reviewed
• [Journal Article] Molecular basis of methanol-inducible gene expression and its application in the methylotrophic yeast Candida boidinii. (2009)
  • Author(s): Yurimoto, H.
  • Journal Title: Biosci. Biotechnol. Biochem.
  • NAID: 10027539388
  • Peer Reviewed
• [Journal Article] Methanol-inducible gene expression and heterologous Protein production in the methylotrophic yeast Candida boidinii. (2009)
  • Author(s): Yurimoto, H. and Sakai, Y.
  • Journal Title: Biotechnol. Appl. Biochem.
  • Peer Reviewed
• [Journal Article] アーキアにおけるホルムアルデヒド固定酵素群の生理機能 (2008)
  • Author(s): 由里本博也, 折田和泉, 阪井康能
  • Journal Title: バイオサイエンスとインダストリー 66 Pages: 447-449
  • NAID: 10024745583
• [Journal Article] Trm1p, a Zn(II)_2Cys_6-type transcription factor, is a master regulator of methanol-specific gene activation in the methylotrophic yeast Candida boidinii (2008)
  • Author(s): Sasano, Y., H. Yurimoto, M. Yanaka, Y. Sakai.
  • Journal Title: Eukaryot. Cell. 7 Pages: 527-536
  • Peer Reviewed
• [Journal Article] アーキアにおけるホルムアルデヒド固定酵素群の生理機能 (2008)
  • Author(s): 由里本博也、折田和泉、阪井康能
  • Journal Title: バイオサイエンスとインダストリー 66 Pages: 447-449
  • NAID: 10024745583
• [Journal Article] Trmlp, a Zn(II)2Cys6-type transcription factor, is a master regulator of methanol-specific gene activation in the methylotrophic yeast Candida boidinii (2008)
  • Author(s): Sasano, Y., et. al.
  • Journal Title: Eukaryot. Cell. 7 Pages: 527-536
  • Peer Reviewed
• [Journal Article] Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase (2007)
  • Author(s): Orita, I., N. Sakamoto, N. Kato, H. Yurimoto, Y. Sakai
  • Journal Title: Appl. Microbiol. Biotechnol. 76 Pages: 439-445
  • Peer Reviewed
• [Journal Article] Gene-tagging mutagenesis in the methylotrophic yeast Candida boidinii (2007)
  • Author(s): Sasano, Y., H. Yurimoto, Y. Sakai
  • Journal Title: J. Biosci. Bioeng. 104 Pages: 86-89
  • NAID: 110006368813
  • Peer Reviewed
• [Journal Article] Gene-tagging mutagenesis in the methylotrophic yeast Candida boidinii (2007)
  • Author(s): Sasano, Y., et. al.
  • Journal Title: J. Biosci. Bioeng. 104 Pages: 86-89
  • Peer Reviewed
• [Journal Article] Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase (2007)
  • Author(s): Orita, I., et. al.
  • Journal Title: Appl. Microbiol. Biotechnol. 76 Pages: 439-445
  • Peer Reviewed