Environmental behavior of polyethylene glycol-degradative megaplasmid of Sphingomonads

• Project/Area Number: 19780060
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: Okayama University
• Principal Investigator: TANI Akio Okayama University, 資源生物科学研究所, 助教 (00335621)
• Research Collaborator: KIMBARA Kazuhide 岡山大学, 資源生物科学研究所, 准教授 (30225122) ; KAWAI Fusako 京都工芸繊維大学, バイオベースマテリアル研究センター, 特任教授 (60118007)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,710,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥2,800,000 (Direct Cost: ¥2,800,000)
• Keywords: ポリエチレングリコール / Sphingomonas属細菌 / プラスミド / xenobiotics / Sphingomonads / plasmid / polyethylene glycol (PEG)

Research Abstract

人工化合物であるポリエチレングリコール(PEG)の有力な分解菌であるSphingomonas属細菌はPEG分解遺伝子をプラスミドにコードしている。このプラスミドは接合伝達で他の菌にも移ることが示唆されているが、その宿主特異性などは明らかではない。本研究ではPEG分解菌が持つプラスミドの環境中での挙動を解析するにあたり基礎的な知見を蓄積し、PEGの完全分解に関わる遺伝子群を明らかにした。

Research Products

• [Journal Article] Involvement of PEG-carboxylate dehydrogenase and glutathione S-transferase in PEG metabolism by Sphingopyxis macrogoltabida strain 103. (2008)
  • Author(s): Somyoonsap, P., Tani, A., Charoenpanich, J., Minami, T., Kimbara, K., Kawai, F.
  • Journal Title: Appl. Microbiol. Biotechnol. 81(3) Pages: 473-84
  • Peer Reviewed
• [Journal Article] Polyethylene glycol (PEG)-carboxylate-CoA synthetase is involved in PEG metabolism in Sphingopyxis macrogoltabida strain 103 (2008)
  • Author(s): Tani, A., Somyoonsap, P., Minami, T., Kimbara, K. & Kawai, F.
  • Journal Title: Arch Microbiol 189 Pages: 407-410
  • Peer Reviewed
• [Journal Article] Involvement of PEG-carboxylate dehydrogenase and glutathione S-transferase in PEG metabolism by Sphingopyxis macrogoltabida strain 103 (2008)
  • Author(s): Somyoonsap, P., Tani. A., Charoenpanich. J., Minami. T., Kimbara. K., Kawai. F
  • Journal Title: Appl. Microbiol. Biotechnol 81(3) Pages: 473-484
• [Journal Article] Polyethylene glycol (PEG)-carboxylate-CoA synthetase is involved in PEG metabolism in Sphingopyxis macrogoltabida strain 103 (2008)
  • Author(s): Tani, A., Somyoonsap, P., Minami, T., Kimbara, K. & Kawai. F
  • Journal Title: Arch Microbiol 189 Pages: 407-410
  • Peer Reviewed
• [Journal Article] Sphingopyxis属細菌によるポリエチレングリコール(PEG)分解機構 (2007)
  • Author(s): 谷明生、河合富佐子
  • Journal Title: 生物工学会誌 Vol85(6) Pages: 267-269
  • NAID: 110006317604
• [Journal Article] Structure and conservation of polyethylene glycol-degradative operon in Sphingomonas species (2007)
  • Author(s): A. Tani, J. Charoenpanich, T. Minami, T. Mori, M. Takeichi, K. Kimbara, and F. Kawai
  • Journal Title: Microbiology 153 Pages: 338-346
  • Peer Reviewed
• [Journal Article] Xenoestrogenic short ethoxy chain nonylphenol is oxidized by a flavoprotein alcohol dehydrogenase from Ensifer sp. strain AS08 (2007)
  • Author(s): X. liu, A. Tani, K. Kimbara, F. Kawai
  • Journal Title: Appl. Microbiol. Biotechnol. 73 Pages: 1414-1422
  • Peer Reviewed
• [Journal Article] Polyethylene glycol (PEG)-carboxylate-CoA synthetase is involved in PEG metabolism in Sphingopyxis macrogoltabida strain 103 (2007)
  • Author(s): Tani A, Somyoonsap P, Minami T, Kimbara K, Kawai F.
  • Journal Title: Arch. Microbiol. DOI 10.1007/s00203-007-0320-z
  • Peer Reviewed
• [Journal Article] Sphingopyxis属細菌によるポリエチレングリコール(PEG)分解機構 (2007)
  • Author(s): 谷 明生、河合富佐子
  • Journal Title: 生物工学会誌 85 Pages: 267-269
  • NAID: 110006317604
• [Presentation] The physiological role of glutathione-S-transferase in the downstream of peg operon in Sphingopyxis macrogoltabida (2007)
  • Author(s): P. Somyoonsap, A. Tani, K. Kimbara, F. Kawai
  • Organizer: 日本生物工学会大会
  • Place of Presentation: 広島大学
  • Year and Date: 2007-09-25
• [Presentation] Xenobiotic polymer degradation by Sphingomonads (2007)
  • Author(s): A. Tani, J. Charoenpanich, P. Somyoonsap, W. Rimwangtragool, K. Kimbara, F. Kawai
  • Organizer: JSPS-NRCT Core university program on Development of thermotolerant microbial resources and their applications, Thailand
  • Place of Presentation: Walailak University, Nakhon Si Thammarat
• [Presentation] The physiological role of glutathione-S-transferase in the downstream of peg operon in Sphingopyxis macrogoltabida (2007)
  • Author(s): P. Somyoonsap, A. Tani, K. Kimbara, F. Kawai
  • Organizer: 日本生物工学会大会
  • Place of Presentation: 広島大学東広島キャンパス総合科学部
• [Presentation] Xenobiotic polymer degradation by Sphingomonads. (2007)
  • Author(s): A. Tani, J. Charoenpanich, P. Somyoonsap, W. Rimwangtragool, K. Kimbara, F. Kawai
  • Organizer: JSPS-NRCT Core university program (1998-2008) on Development of thermotolerant microbial resources and their applications
  • Place of Presentation: Walailak University, Thailand