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Isolation of microorganism and characterization of its metabolism under different oxygen concentrations

Research Project

Project/Area Number 19780065
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionKyoto Gakuen University

Principal Investigator

SHINODA Yoshifumi  Kyoto Gakuen University, バイオ環境学部, 講師 (20434657)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,760,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywords微生物機能 / 環境応答 / 生物 / 発現制御 / 低酸素 / スクリーニング / 微生物
Research Abstract

微好気性細菌Magnetospirillum sp. TS-6株を異なる酸素濃度条件下で培養し、安息香酸の好気/嫌気代謝の鍵となる3つの酵素の発現を調べた。その結果、1)好気条件と嫌気条件の両方の代謝経路で機能する安息香酸CoAリガーゼ(Bcl)の遺伝子は各条件下でほぼ一定の転写量を示した 2)好気代謝を担うベンゾイルCoAオキシゲナーゼ(Box)の遺伝子は酸素濃度0%とそれ以外でゼロ/サムになるように転写されていた 3)嫌気代謝を担うベンゾイルCoAレダクターゼ(Bcr)の遺伝子は、酸素濃度が減少するにつれて転写量が連続的に増加した。これにより、微好気環境に生息する環境微生物の特徴的な環境応答の一端を明らかにした。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (6 results)

All 2008 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (1 results) Remarks (3 results)

  • [Journal Article] 6-Oxocyclohex-1-ene-1-carbonyl-coenzyme A hydrolases from obligately anaerobic bacteria : characterization and identification of its gene as a functional marker for aromatic compounds degrading anaerobes.2008

    • Author(s)
      Kuntze, K., Shinoda, Y., Moutakki, H., McInerney, M. J., Vogt, C., Richnow, H.H., Boll, M.
    • Journal Title

      Environmental Microbiology 10

      Pages: 1547-1556

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 6-Oxocyclohex-1-ene-1-carbonyl-coenzyme A hydrolases from obligately anaerobic b acteria : characterization and identification of its gene as a functional marker for aromatic compounds degrading anaerobes2008

    • Author(s)
      Kuntze, K., Shinoda, Y., Moutakki, H., McInerney, M. J., Vogt, C., Rich now, H. H., Boll, M.
    • Journal Title

      Environmental Microbiology 10

      Pages: 1547-1556

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Magnetospirillum sp. TS-6株の安息香酸分解遺伝子の発現に対する曝気条件の影響2008

    • Author(s)
      篠田吉史、谷吉樹、加藤暢夫
    • Organizer
      2008年度日本農芸化学会大会
    • Place of Presentation
      名城大学
    • Year and Date
      2008-03-26
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.kyotogakuen.ac.jp/~microbio

    • Related Report
      2008 Final Research Report
  • [Remarks]

    • URL

      http://www.kyotogakuen.ac.jp/~microbio/

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.kyotogakuen.ac.jp/~microbio/

    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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