Studies on the elicitation of sweetness of sweet-tasting proteins.
| Project/Area Number |
19780074
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
MASUDA Tetsuya Kyoto University, 大学院・地球環境学堂, 助教 (80311744)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,280,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | 甘味タンパク質 / ソーマチン / 結晶構造解析 / 甘味 / 変異体 / Pichia pastoris / X線結晶構造解析 / 放射光 |
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| Research Abstract |
Thaumatin is an intensely sweet-tasting protein. To clarify the structure-sweetness relationships in thaumatin, we performed high resolution crystal structure analysis. The results showed that overall structures of these mutants are identical to that of plant thaumatin. However the subtle conformational changes in the main chain of K67 were induced by mutation of R82. The surface electric potential on the cleft containing side of the thaumatin was dramatically changed by the mutation at R82. These results demonstrated that the strict surface electrostatic potentials at the specific site formed by R82 and K67 are required for thaumatin sweetness
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Cloning of the thaumatin I cDNA and characterization of recombinant thaumatin I secreted Pichia pastoris2007
Author(s)
Ide, N., Kaneko, R., Wada, R., Metha, A., Tamaki, S., Tsuruta, T., Fu j ita, Y., Masuda, T., Kitabatake, N.
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Journal Title
Biotechnol. prog 23
Pages: 1023-1030
Related Report
Peer Reviewed
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[Journal Article] Cloning of the thaumatin I cDNA and characterization of recombinant thaumatin I secreted Pichia pastoris.2007
Author(s)
Ide, N., Kaneko, R., Wada, R., Metha, A., Tamaki, S., Tsuruta,
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Journal Title
Biotechnol. Prog 23
Pages: 1023-1030
Related Report
Peer Reviewed
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[Presentation] リゾチームの甘味特性2007
Author(s)
桝田哲哉、井出信幸、北畠直文
Organizer
日本食品科学工学会, 関西支部大会
Place of Presentation
京都大学
Year and Date
2007-11-09
Related Report
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