A role of actin in maturation process of a marine alga Porphyra and its application to a molecular marker

• Project/Area Number: 19780141
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General fisheries
• Research Institution: Hokkaido University
• Principal Investigator: KITADE Yukihiro Hokkaido University, 大学院・水産科学研究院, 特任准教授 (90399999)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,090,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: 海苔 / スサビノリ / 成熟 / アクチン / 分子マーカー / 紅藻 / 遺伝子ファミリー / 有性生殖

Research Abstract

海面養殖において成熟の進んだ海苔の収穫は製品の質を低下させることが知られている。しかし、海苔の成熟は比較的広い環境条件下で起こるため成熟時期の予測は難しい。そこで、海苔の成熟をモニターし得る何らかの客観的な指標があれば、適正な漁期を判断する基準の一つとして役立つと考えられる。本研究では海苔の持つ4種類のアクチン遺伝子の発現様式の違いに注目し、そのうちの1種類が成熟の指標として適していることを分子生物学的手法により明らかにした。

Research Products

• [Journal Article] Structural features and gene-expression profiles of actin homologs in Porphyra yezoensis (Rhodophyta) (2008)
  • Author(s): Kitade Y, Nakamura M, Uji T, Fukuda S, Endo H, Saga N.
  • Journal Title: Gene 423 Pages: 79-84
• [Journal Article] Simple differentiation of two closely related species Porphyra tenera and Porphyra yezoensis (Bangiophyceae, Rhodophyta) based on length polymorphism of actin-related protein 4 gene (ARP4) (2008)
  • Author(s): Park F-J, Endo H, Kitade Y, Saga N
  • Journal Title: Fisheries Science 74 Pages: 613-620
  • NAID: 10020861052
  • Peer Reviewed
• [Journal Article] Identification of genes preferentially expressed during asexual sporulation in Porphyra yezoensis gametophytes (Bangiales, Rhodophyta) (2008)
  • Author(s): Kitade Y, Asamizu E, Fukuda S, Nakajima M, Ootsuka S, Endo H, Tabata S, Saga N
  • Journal Title: Journal of Phycology 44 Pages: 113-123
  • Peer Reviewed
• [Journal Article] Structural features and gene-expression profiles of actin homologs in Porphyra yezoensis(Rhodophyta) (2008)
  • Author(s): Kitade Y., Nakamura M., Uji T., Fukuda S., Endo H., Saga N.
  • Journal Title: Gene 423 Pages: 79-84
  • Peer Reviewed
• [Journal Article] Simple differentiation of two closely related species Porphyra tenera and Porphyra yezoensis(Bangiophyceae, Rhodophyta)based on length polymorphism of actin-related protein 4 gene(ARP4) (2008)
  • Author(s): Park E.-J., Endo H., Kitade Y., Saga N.
  • Journal Title: Fisheries Science 74 Pages: 613-620
  • NAID: 10020861052
  • Peer Reviewed
• [Journal Article] Identification of genes preferentially expressed during asexual sporulation in Porphyra yezoensis gametophytes (Bangiales, Rhodophyta) (2008)
  • Author(s): Kitade Y., Asamizu E., Fukuda S., Nakajima M., Ootsuka S., Endo H., Tabata S., Saga N.
  • Journal Title: Journal of Phycology 44 Pages: 113-123
  • Peer Reviewed
• [Presentation] 紅藻スサビノリ配偶体の成熟をモニターする分子マーカーの探索 (2007)
  • Author(s): 北出幸広, 中村理湖, 宇治利樹, 福田覚, 遠藤博寿, 嵯峨直恆
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14