Studies on the specific binding of vitellogenin and very-low-density lipoprotein to ovarian lipoprotein receptor(s) in teleosts

• Project/Area Number: 19780143
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General fisheries
• Research Institution: Hokkaido University
• Principal Investigator: HIRAMATSU Naoshi Hokkaido University, 大学院・水産科学研究院, 助教 (10443920)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,810,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥510,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: ビテロジェニン / 受容体 / リポ蛋白 / 卵形成 / 魚類

Research Abstract

本研究は、魚卵中の卵黄についての研究であり、特に卵黄の素となる血液中の前駆体(ビテロジェニンと超低密度リポ蛋白質)が、どの様に卵へ取り込まれるかを調べた。スズキ類とボラ類の魚を使いこれらの蛋白質や遺伝子を解析した結果、まずビテロジェニンには3種類のサブタイプがあることが明らかとなった。また各種ビテロジェニンと超低密度リポ蛋白質は、それぞれ異なる受容体と結合してから卵の中に取り込まれる可能性が初めて示された。

Research Products

• [Journal Article] Multiple vitellogenin derived yolk proteins in grey mullet (Mugil cephalus): disparate proteolytic patterns associated with ovarian follicle maturation (2008)
  • Author(s): Amano Haruna, Fujita Toshiaki, Hiramatsu Naoshi, Kagawa Hirohiko, Matsubara Takahiro, Sullivan V. Craig, Hara Akihiko
  • Journal Title: Mol. Reprod. Dev 75(8) Pages: 1307-1317
  • Peer Reviewed
• [Journal Article] Molecular alteratin of three forms of vitellogenins and their poduct yolk proteins during oocyte growth and maturation in grey mullet (Mugil cephalus) (2008)
  • Author(s): Amano Haruna, Fujita Toshiaki, Hiramatsu Naoshi, Kagawa Hirohiko, Sawaguchi Sayumi, Matsubara Takahiro, Sullivan V. Craig, Hara Akihiko
  • Journal Title: Cybium 32(2) suppl Pages: 156-158
  • Peer Reviewed
• [Journal Article] Multiple vitellogenin-derived yolk proteins in gray mullet(Mugil cephalus) : disparate proteolytic patterns associated with ovarian follicle maturation (2008)
  • Author(s): Haruna Amano
  • Journal Title: Molecular Reproduction and Development 75 Pages: 1307-1317
  • Peer Reviewed
• [Journal Article] Molecular alteration of three forms of vitellogenins and their product yolk proteins during oocyte growth and maturation in grey mullet(Mugil cephalus) (2008)
  • Author(s): Haruna Amano
  • Journal Title: Cybium 32(2) Pages: 156-158
  • Peer Reviewed
• [Journal Article] Egg yolk proteins in grey mullet(Mugil cephalus):purification and classification of multiple lipovitellins and other vitellogenin-derived yolk prteins and molecular cloning of the parent vitellogenin genes (2007)
  • Author(s): Amano Haruna, Fujita Toshiaki, Hiramatsu Naoshi, Shimizu Munetaka, Sawaguchi Sayumi, Matsubara Takahiro, Kagawa Hirohiko, Nagae Masaki, Sullivan V. Craig, Hara Akihiko
  • Journal Title: J. Exp. Zool 307A Pages: 324-341
  • Peer Reviewed
• [Journal Article] Purification of multiple vitellogenins ingrey mullet (Mugil cephalus) (2007)
  • Author(s): Amano Haruna, Fujita Toshiaki, Hiramats Naoshi, Sawaguchi Sayumi, Matsubara Takahiro, Sullivan V. Craig, Hara Akihiko
  • Journal Title: Mar. Biol 152 Pages: 1215-1225
  • Peer Reviewed
• [Journal Article] Egg yolk proteins in grey mullet (Mugil cephalus): purification and classification of multiple lipovitellins and other vitellogenin-derived yolk proteins and molecular cloning of the parent vitellogenin genes. (2007)
  • Author(s): Haruna Amano
  • Journal Title: J. Experimental Zoology 307A Pages: 324-341
  • Peer Reviewed
• [Journal Article] Purification of multiple vitellogenins in grey mullet (Mugil cephalus). (2007)
  • Author(s): Haruna Amano
  • Journal Title: Marine Biology 152 Pages: 1215-1225
  • Peer Reviewed
• [Presentation] Oogenesis in temperate basses (Genus Morone): Multiple yolk precursors (vitellogenins) and their receptors (2009)
  • Author(s): Reading J. Benjamin, Hiramats Naoshi, Sullivan V. Craig
  • Organizer: 21st COE Program Special Lecture
  • Place of Presentation: Hakodate, Hokkaido
  • Year and Date: 2009-01-27
• [Presentation] Oogenesis in temperate basses(Genus Morone) : Multiple yolk precursors(vitellogenins)and their receptors (2009)
  • Author(s): Benjamin J. Reading
  • Organizer: 21^<st> COE program special lecture
  • Place of Presentation: 函館、北海道大学
  • Year and Date: 2009-01-27
• [Presentation] 魚類のリポ蛋白と受容体に関る研究 (2008)
  • Author(s): 平松尚志
  • Organizer: 北海道海洋生物科学研究会シンポジウム
  • Place of Presentation: 函館、北海道
  • Year and Date: 2008-11-07
• [Presentation] 魚類のリポ蛋白質と受容体に関する研究 (2008)
  • Author(s): 平松尚志
  • Organizer: 北海道海洋生物科学研究会シンポジウム
  • Place of Presentation: 函館、北海道大学
  • Year and Date: 2008-11-07
• [Presentation] Yolk assembly in teleost: recent findings on the deposition of ovarian lipids and proteins (2008)
  • Author(s): Hiramats Naoshi, Todo Takashi, Ito Takahiro, Massaki Kiyohiro, Kasahara Ayumi, Amano Haruna, Reading J. Benjamin, Matsubara Takahiro, Sawaguchi Sayumi, Sullivan.V. Craig, Hara Akihiko
  • Organizer: The Annual International Conference and Exposition of World Aquaculture Society, World Aquaculture
  • Place of Presentation: Busan, Korea
  • Year and Date: 2008-05-23
• [Presentation] Yolk assembly in teleost : recent findings on the deposition of ovarian lipids and proteins (2008)
  • Author(s): Naoshi Hiramatsu
  • Organizer: The annual international conference and exposition of world aquaculture society, World aquaculture 2008
  • Place of Presentation: Busan, Korea
  • Year and Date: 2008-05-23
• [Presentation] Multiple vitellogenins and their derived yolk proteins in grey mullet (Mugil cephalus): Differential proteolytic patterns during oocyte growth and maturation (2007)
  • Author(s): Amano Haruna, Fujita Toshiaki, Hiramatsu Naoshi Matsubara Takahiro, Sullivan V.Craig, Hara Akihiko
  • Organizer: 4th Japan-Korea, Korea-Japan Joint Meeting on Reproductive Biology of Aquatic animals
  • Place of Presentation: Nagasaki University, Nagasaki
• [Presentation] Purification and classification of egg yolk proteins derivedfrom multiple vitellogenins in grey mllet (Mugil cephalus) (2007)
  • Author(s): Amano Haruna, Fujita Toshiaki, Hiramtsu Naoshi, Matsubara Takahiro, Sullivan V. Craig, Hara Akihiko
  • Organizer: 8th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: St. Malo, France
• [Presentation] Yolk precursors in white perch (Morone americana): deduced primary structures of three types of vitellogenin (Vg) proteins and disparate binding of the different Vgs to multiple ovarian receptors (2007)
  • Author(s): Reading J. Benjamin, Hiramtsu Naoshi, Matsubara Takahiro, Hara Akihiko, Sullivan V. Craig
  • Organizer: 8th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: St. Malo, France
• [Presentation] Multiple vitellogenins and their derived yolk proteins in grey mullet (Mugil cephalus): differential proteolytic patterns during oocyte growth and maturation. (2007)
  • Author(s): Haruna Amano
  • Organizer: 4th Japan-Korea, Korea-Japan Joint Meeting on Reproductive Biology of Aquatic Animals
  • Place of Presentation: 長崎大学
• [Presentation] Purification and classification of egg yolk proteins derived from multiple vitellogenins in grey mullet (Mugil cephalus). (2007)
  • Author(s): Haruna Amano
  • Organizer: 8th International Symposium on Reproductive Physiology of Fish.
  • Place of Presentation: St. Malo, France
• [Presentation] Yolk precursors in white perch (Morone Americana): deduced primary structures of three types of vitellogenin (Vg) proteins and disparate binding of the different Vgs to multiple ovarian receptors. (2007)
  • Author(s): Benjamin J. Reading
  • Organizer: 8th International Symposium on Reproductive Physiology of Fish.
  • Place of Presentation: St. Malo, France
• [Remarks]
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• [Remarks]
  • URL: http://www.geocities.co.jp/hlaboratory/
• [Remarks]
  • URL: http://www.geocities.co.jp/hlaboratory/