| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥600,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Research Abstract |
The effects of high-hydrostatic pressure (HHP) on agroproducts and soybean cotyledon as a cellular biological material were investigated from the viewpoints of the cell structure and enzyme reaction system. Damage to cell structure was evaluated by measuring dielectric properties using the Cole-Cole arc, the radius of which decreased as pressure level increased. Results suggested that cell structure was damaged by HHP. The distribution of free amino acids was measured after HHP (200MPa) of soybean soaked in water or sodium glutamate (Glu) solution. HHP resulted in high accumulation of free amino acids in water-soaked soybean, due to proteolysis. HHP of soybean in Glu solution caused higher accumulation of γ-aminobutyric acid, suggesting that both proteolysis and specific Glu metabolism were accelerated by HPP. We concluded that HHP partially degraded cell structure and accelerated biochemical reactions by allowing enzyme activities to remain. These events can be considered "high-pressure induced transformation" of soybean.
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