An approach to establish a method that allows efficient screening of beneficial probiotic strains for livestock.

• Project/Area Number: 19780198
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Zootechnical science/Grassland science
• Research Institution: Kyoto Prefectural University (2008-2009); Hokkaido University (2007)
• Principal Investigator: INOUE Ryo Kyoto Prefectural University, 大学院・生命環境科学研究科, 講師 (70443926)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,860,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥660,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,000,000 (Direct Cost: ¥1,000,000)
• Keywords: プロバイオティクス / 上皮細胞 / ブタ / インターロイキン12 / 腸管上皮細胞 / Caco-2

Research Abstract

Effect of probiotics was evaluated with consideration of "effect on intestinal epithelial cells" and "host specificity". This study showed most probiotic lactic acid bacteria (LAB) did not affect considerably on gene expression of intestinal epithelial cells under physical condition but not inflammatory status. Additionally no host specificity was found at least in in vitro co-culture system of splenocyte, mesenteric lymph node tissue with LAB. Thus, probiotic strains effective on pigs could be screened by using mouse cells.

Research Products

• [Journal Article] Diverse Immune Responses to Orally Administrated Heat-Killed Cell Preparation of Enterococcus faecalis Strain EC-12 in Immune Tissues in Mice. (2009)
  • Author(s): Yoshikawa, T. Inoue, R. Yoshida, Y. Watanabe, T. Bukawa, W., Ushida, K.
  • Journal Title: Bioscience Biotechnology and Biochemistry 73(6) Pages: 1439-42
• [Journal Article] Diverse Immune Responses to Orally Administered Heat-Killed Cell Preparation of Enterococcus faecalis Strain EC-12 in Murine Immune Tissues (2009)
  • Author(s): T.Yoshikawa, R.Inoue, Y.Yoshida, T.Watanabe, K.Ushida
  • Journal Title: Bioscience, Biotechnology, and Biochemistry 73 Pages: 1439-1442
  • NAID: 10027542884
  • Peer Reviewed
• [Presentation] 乳酸菌のIL-12産生誘導能に関する研究 (2010)
  • Author(s): 梛野崇之
  • Organizer: 第64回栄養食糧学会
  • Place of Presentation: アスティ徳島
  • Year and Date: 2010-05-23
• [Presentation] 乳酸菌死菌体によるマウスマクロファージサイトカイン生産誘導 (2010)
  • Author(s): 野宮一平, 星野剛, 井上亮, 渡辺卓巳, 牛田一成
  • Organizer: 農芸化学会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-03-28
• [Presentation] 乳酸菌のIL-12産生誘導能に関する研究 (2009)
  • Author(s): 梛野崇之
  • Organizer: 第15回Hind gut club Japan
  • Place of Presentation: 専修大学
  • Year and Date: 2009-12-05
• [Presentation] 殺菌乳酸菌体EC-12投与に対する各種免疫組織の応答の差異 (2009)
  • Author(s): 吉川徹
  • Organizer: 日本食品免疫学会2009年度大会
  • Place of Presentation: 東京大学