Recovery of Hydrogen energy from cow waste by anaerobic microflora naturally present in the waste

• Project/Area Number: 19780207
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Zootechnical science/Grassland science
• Research Institution: National Agricultural Research Organization
• Principal Investigator: YOKOYAMA Hiroshi National Agricultural Research Organization, 畜産草地研究所・資源化システム研究チーム, 主任研究員 (40391370)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,080,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥780,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥700,000 (Direct Cost: ¥700,000)
• Keywords: 水素 / バイオマス / 嫌気性好熱細菌 / 牛糞 / 資源循環 / 嫌気性細菌 / 食品残渣 / 畜産バイオマス / 発酵 / 水素発酵 / 家畜排泄物 / 複合微生物 / 菌叢解析 / 水素生産細菌

Research Abstract

Hydrogen (700 mL/kg-cow dung) was produced by dry fermentation of cow dung at a optimum temperature of 60℃ using hydrogen-producing bacteria, naturally present in the dung. Extremely thermophilic microflora, enriched from cow dung, showed a high hydrogen yield of 3.32 mol/mol-glucose at the HRT of 3 days in continuous culture. The microflora was suggested to posses unusual fermentative properties, as compared with moderately thermophilic and mesophilic bacteria.

Research Products

• [Journal Article] Continuous hydrogen production from glucose by using extreme thermophilic anaerobic microflora (2009)
  • Author(s): Yokoyama H, Ohmori H, Waki M, Ogino A, Tanaka Y.
  • Journal Title: Journal of Bioscience and Bioengineering vol.107 Pages: 64-66
  • NAID: 110007042023
  • Peer Reviewed
• [Journal Article] Continuous hydrogen production from glucose by using extreme thermophilic anaerobic microflora (2009)
  • Author(s): Yokoyama H, Ohmori H, Waki M, Ogino A, Tanaka Y.
  • Journal Title: J. Biosci. Bioengi. 107 Pages: 64-66
  • NAID: 110007042023
• [Journal Article] Continuous hydrogen production from glucose by using extreme thermophilic anaerobic microflora (2009)
  • Author(s): Yokoyama H, Ohmori H, Waki M, Ogino A, Tanaka Y.
  • Journal Title: Journal of Bioscience and Bioengineering 107 Pages: 64-66
  • NAID: 110007042023
  • Peer Reviewed
• [Journal Article] Hydrogen fermentation properties of undiluted cow dung (2007)
  • Author(s): Yokoyama H, Waki M, Ogino A, Ohmori H, Tanaka Y
  • Journal Title: Journal of Bioscience and Bioengineering vol.104 Pages: 82-85
  • NAID: 110006368812
  • Peer Reviewed
• [Journal Article] Community analysis of hydrogen-producing extreme thermophilic anaerobic microflora enriched from cow manure with five substrates (2007)
  • Author(s): Yokoyama H, Moriya N, Ohmori H, Waki M, Ogino A, TanakaY
  • Journal Title: Applied Microbiology and Biotechnology vol., 77 Pages: 213-222
  • Peer Reviewed
• [Journal Article] Hydrogen fermentation properties of undiluted cow dung (2007)
  • Author(s): Yokoyama H, Waki M, Ogino A, Ohmori H, Tanaka Y
  • Journal Title: J. Biosci. Bioengi. 104 Pages: 82-85
  • NAID: 110006368812
• [Journal Article] Community analysis of hydrogen-producing extreme thermophilic anaerobic microflora enriched from cow manure with five substrates (2007)
  • Author(s): Yokoyama H, Moriya N, Ohmori H, Waki M, Ogino A, TanakaY
  • Journal Title: Appl. Microbiol. Biotechnol. 77 Pages: 213-222
• [Journal Article] Hydrogen fermentation properties of undiluted cow dung (2007)
  • Author(s): Yokoyama H, Waki M, Ogino A, Ohmori H, Tanaka Y
  • Journal Title: Journal of Bioscience and Bioengineering 104 Pages: 82-85
  • NAID: 110006368812
  • Peer Reviewed
• [Journal Article] Community analysis of hydrogen-producing extreme thermophilic anaerobic microflora enriched from cow manure with five substrates (2007)
  • Author(s): Yokoyama H, Moriya N, Ohmori H, Waki M, Ogino A, Tanaka Y
  • Journal Title: Applied Microbiology and Biotechnology 77 Pages: 213-222
  • Peer Reviewed
• [Presentation] 内在性水素生産細菌を利用した牛糞の水素発酵 (2007)
  • Author(s): 横山浩、荻野暁史、和木美代子、田中康男
  • Organizer: 第59回日本生物工学会大会
  • Place of Presentation: 広島大学