Involvement of miRNA in mammary gland differentiation

• Project/Area Number: 19780208
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied animal science
• Research Institution: The University of Tokyo
• Principal Investigator: NAGAOKA Kentaro The University of Tokyo, 大学院農学生命科学研究科, 助教 (60376564)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,790,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: miRNA / Cox-2 / 乳腺分化 / 細胞増殖 / mir-101a / 乳腺退行 / AUF1 / 転写後調節 / c-myc

Research Abstract

哺乳動物は妊娠すると、ミルクを作るために乳腺細胞の増殖、分化そして退行を繰り返す。その遺伝子発現は精密に制御されているが、逸脱が生じるとミルク合成量の低下や乳癌の発生などにつながる。本研究により、近年注目を集めているマイクロRNA の一つが、細胞増殖を刺激する遺伝子発現を抑制させ、間接的にマウス乳腺細胞の増殖を制御する事が明らかとなった。今後、より詳細な研究を行うことで、乳腺機構の理解を深めることが可能となろう。

Research Products

• [Journal Article] Induction of endogenous tau interferon gene transcription by CDX2 and high acetylation in bovine non-trophoblast cells (2009)
  • Author(s): Sakurai T, Sakamoto A, Muroi Y, Bai H, Nagaoka K, Tamura K, Takahashi T, Hashizume K, Sakatani M, Takahashi M, Godkin JD, Imakawa K
  • Journal Title: Biology of Reproduction 80 Pages: 1223-1231
  • Peer Reviewed
• [Journal Article] Interleukin-1 receptor antagonist 1 expression in the equine endometrium during peri-implantation period (2009)
  • Author(s): Haneda S, Nagaoka K, Nambo Y, Kikuchi M, Nakano Y, Matsui M, Miyake Y, Macleod JE, Imakawa K
  • Journal Title: Domestic Animal Endocrinology 36 Pages: 209-218
  • Peer Reviewed
• [Journal Article] MiR-101a controls cell proliferation by regulating cyclooxygenase-2 during mammary gland development (2009)
  • Author(s): Tanaka T, Imakawa K, Sakai S, Nagaoka K
  • Journal Title: Differentiation 77 Pages: 181-187
  • Peer Reviewed
• [Journal Article] Differences in adrenocortical secretory and gene expression responses to stimulation in vitro by ACTH or prolactin between high and low avoidance Hatano rats (2009)
  • Author(s): Jaroenporn S, Nagaoka K, Ohta R, Shirota M, Watanabe G, Taya K
  • Journal Title: Stress 12 Pages: 22-29
  • Peer Reviewed
• [Journal Article] MiR-101a controls cell proliferation by regulating cyclooxyg enase-2 during mammary gland development (2009)
  • Author(s): Tanaka T, Imakawa K, Sakai S, Nagaoka K
  • Journal Title: Differentiation 77 Pages: 181-187
  • Peer Reviewed
• [Journal Article] Production of hydrogen peroxide by a small molecular mass compound in milk from Holstein cows with high and low milk somatic cell count. (2008)
  • Author(s): Sakai S, Nonobe E, Satow T, Imakawa K, Nagaoka K
  • Journal Title: Journal of Dairy Research 75 Pages: 335-339
  • Peer Reviewed
• [Journal Article] Generation of hydrogen peroxide by a low molecular weight compound in whey of Holstein dairy cows (2008)
  • Author(s): Sakai S, Satow T, Imakawa K, Nagaoka K
  • Journal Title: Journal of Dairy Research 75 Pages: 257-261
  • Peer Reviewed
• [Journal Article] Comparative effects of prolactin versus ACTH, estradiol, progesterone, testosterone, and dihydrotestosterone on cortisol release and proliferation of the adrenocortical carcinoma cell line H295R (2008)
  • Author(s): Jaroenporn S, Furuta C, Nagaoka K, Watanabe G, Taya K
  • Journal Title: Endocrine 33 Pages: 205-209
  • Peer Reviewed
• [Journal Article] Production of hydrogen peroxide by a small molecular mass co mpound in milk from Holstein cows with high and low milk som atic cell count (2008)
  • Author(s): Sakai S, Nonobe E, Satow T, Imakawa K, Nagaoka K
  • Journal Title: Journal of Dairy Research 75 Pages: 335-339
  • Peer Reviewed
• [Journal Article] Generation of hydrogen peroxide by a low molecular weight co mpound in whey of Holstein dairy cows (2008)
  • Author(s): Sakai S, Satow T, Imakawa K, Nagaoka K
  • Journal Title: Journal of Dairy Research 75 Pages: 257-261
  • Peer Reviewed
• [Journal Article] Comparative effects of prolactin versus ACTH, estradiol, pro gesterone, testosterone, and dihydrotestosterone on cortisol release and proliferation of the adrenocortical carcinoma cell line H295R (2008)
  • Author(s): Jaroenporn S, Furuta C, Nagaoka K, Watanabe G, Taya K
  • Journal Title: Endocrine 33 Pages: 205-209
  • Peer Reviewed
• [Journal Article] Physiological roles of prolactin in the adrenocortical response to acute restraint stress (2007)
  • Author(s): Jaroenporn S, Nagaoka K, Kasahara C, Ohta R, Watanabe G, Taya K
  • Journal Title: Endocrine Journal 54 Pages: 703-711
  • NAID: 10020092733
  • Peer Reviewed
• [Journal Article] Direct effects of prolactin on adrenal steroid release in male Hatano high-avoidance (HAA) rats may be mediated through Janus kinase 2 (Jak2) activity (2007)
  • Author(s): Jaroenporn S, Nagaoka K, Ohta R, Watanabe G, Taya K
  • Journal Title: Journal of Reproduction and Development 53 Pages: 887-893
  • NAID: 10020004162
  • Peer Reviewed
• [Journal Article] Involvement of RNA binding proteins AUF1 in mammary gland differentiation (2007)
  • Author(s): Nagaoka K, Tanaka T, Imakawa K, Sakai S
  • Journal Title: Experimental Cell Research 313 Pages: 2937-2945
  • Peer Reviewed
• [Journal Article] Involvement of RNA Binding proteins AUF1 in mammary gland differentiation. (2007)
  • Author(s): Nasaoka K, Tanaka T, Imakawa K, Sakai S
  • Journal Title: Experimental Cell Research (Corresponding author) 313 Pages: 2937-2945
  • Peer Reviewed
• [Journal Article] Physiological roles of prolactin in the adrenocortical response to acute restraint stress. (2007)
  • Author(s): Jaroenporn S, Nagaoka K, Kasahara C, Ohta R, Watanabe G, Taya K
  • Journal Title: Endocrine Journal 54 Pages: 703-711
  • NAID: 10020092733
  • Peer Reviewed
• [Journal Article] Direct effects of prolactin on adrenal steroid release in male Hatano high-avoidance (HAA) rats may be mediated through Janus kinase 2 (Jak2) a ctivity. (2007)
  • Author(s): Jaroenporn S, Nagaoka K, Ohta R, Watanabe G, Taya K
  • Journal Title: Journal of Reprdduction and Development 53 Pages: 887-893
  • Peer Reviewed
• [Journal Article] Prolactin inducesphosphorylation of the STAT5 in adrenal glands of Hatano rats during stress
  • Author(s): aroenporn S, Nagaoka K, Ohta R, Watanabe G, Taya K
  • Journal Title: Life Sci (In press)
  • Peer Reviewed
• [Journal Article] L-amino acid oxidase plays a crucial role in host defense in the mammary glands
  • Author(s): Nagaoka K, Aoki F, Hayashi M, Muroi Y, Sakurai T, Itoh K, Ikawa M, Okabe M, Imakawa K, Sakai S
  • Journal Title: FASEB Journal (In press)
  • Peer Reviewed
• [Presentation] ミルク中抗菌タンパクLAOノックアウトマウスの解析 (2008)
  • Author(s): 永岡謙太郎, 他5名
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2008-09-20
• [Presentation] ミルク中L-amino acid oxidase (LAO)による乳腺感染防御機構 (2008)
  • Author(s): 永岡謙太郎、青木不学、伊川正人、岡部勝、今川和彦、酒井仙吉
  • Organizer: 第101回日本繁殖生物学会大会
  • Place of Presentation: 福岡
• [Presentation] ミルク中抗菌タンパクLAOノックアウトマウスの解析 (2008)
  • Author(s): 永岡謙太郎、青木不学、伊川正人、岡部勝、今川和彦、酒井仙吉
  • Organizer: 日本畜産学会第109回大会
  • Place of Presentation: 水戸
• [Presentation] 乳腺細胞分化過程におけるRNA 結合タンパクAUF1の関与 (2007)
  • Author(s): 永岡謙太郎、田中哲也、今川和彦、酒井仙吉
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] マウス乳腺の退行過程におけるmicroRNA発現変化 (2007)
  • Author(s): 田中哲也、永岡謙太郎、今川和彦、酒井仙吉
  • Organizer: 日本畜産学会107回大会
  • Place of Presentation: 麻布大学
• [Presentation] 乳腺細胞分化過程におけるRNA結合タンパクAUF1の関与 (2007)
  • Author(s): 永岡謙太郎・田中哲哉・今川職彦・酒井仙吉
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] マウス乳腺の退行過程におけるmicroRNA発現変化 (2007)
  • Author(s): 田中哲哉・永岡謙太郎・今川和彦・酒井仙吉
  • Organizer: 日本畜産学会
  • Place of Presentation: 麻布大学