The effect of donor cell characteristic to the ability of nuclear transfer embryonic stem cell lines.

• Project/Area Number: 19780213
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied animal science
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: MIZUTANI Eiji The Institute of Physical and Chemical Research, ゲノム・リプログラミング研究チーム, 研究員 (80443034)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,790,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: 発生工学 / 核移植 / 胚性幹細胞 / 初期化 / 加齢 / 生殖細胞 / 繁殖 / クローン / 初期胚発生

Research Abstract

クローン胚由来胚性幹細胞(ntES細胞)に与えるドナー細胞の性質を調べるため、超高齢マウス5匹の尾から線維芽細胞を採取し核移植を行い、全ての個体からntES細胞を樹立することに成功した。これらのntES細胞は全てES細胞マーカーに陽性であり、キメラマウスを介して、全てのドナー個体由来の産仔作出に成功した。次にこれらntES細胞をドナー核として核移植を行った結果、2年9カ月齢という非常に高齢な個体からクローンマウスを作出することに成功した。

Research Products

• [Journal Article] Therapeutic cloning in individual parkinsonian mice. (2008)
  • Author(s): Tabar V.、水谷英二(11名中7番目)
  • Journal Title: Nature Medicine 14巻4号 Pages: 379-81
  • Peer Reviewed
• [Journal Article] Propagation of senescent mice using nuclear transfer embryonic stem cell lines. (2008)
  • Author(s): 水谷英二、小野哲男、李羽中、牧-、末次里奈子、若山照彦
  • Journal Title: Genesis 46巻9号 Pages: 478-83
  • Peer Reviewed
• [Journal Article] Nuclear transfer preserves the nuclear genome of freeze-dried mouse cells. (2008)
  • Author(s): 小野哲男、水谷英二、李羽中、若山照彦
  • Journal Title: The Journal of Reproduction and Development 54巻6号 Pages: 486-91
  • NAID: 10024940504
  • Peer Reviewed
• [Journal Article] Production of healthy cloned mice from bodies frozen at -20 degrees C for 16 years. (2008)
  • Author(s): 若山清香、水谷英二(7名中4番目)
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America 105巻45号 Pages: 17318-22
  • Peer Reviewed
• [Journal Article] Production of healthy cloned mice from bodies frozen at-20 degrees C for 16 years (2008)
  • Author(s): Wakayama S
  • Journal Title: Proceedings of the National Academy : of Sciences of the United States of America 105巻45号 Pages: 17318-22
  • Peer Reviewed
• [Journal Article] Nuclear transfer preserves the nuclear genome of freeze-dried mouse cells (2008)
  • Author(s): Ono T
  • Journal Title: The Journal of Reproduction and Development 54巻6号 Pages: 486-91
  • Peer Reviewed
• [Journal Article] Propagation of senescent mice using nuclear transfer embryonic stem cell lines (2008)
  • Author(s): Mizutani E
  • Journal Title: Genesis 46巻9号 Pages: 478-83
  • Peer Reviewed
• [Journal Article] Therapeutic cloning in individual parkinsonian mice (2008)
  • Author(s): Tabar V.
  • Journal Title: Nature Medicine 14巻4号 Pages: 379-81
  • Peer Reviewed
• [Journal Article] Efficient establishment of mouse embryonic stem cell lines from single blastomeres and polar bodies (2007)
  • Author(s): 若山清香、水谷英二(7名中6番目)
  • Journal Title: Stem Cells 25巻4号 Pages: 986-993
  • Peer Reviewed
• [Journal Article] Spindle formation and microtubule organization during first division in reconstructed rat embryos produced by somatic cell nuclear transfer. (2007)
  • Author(s): 富岡郁夫、水谷英二(7名中2番目)
  • Journal Title: Journal of Reproduction and Development 53巻4号 Pages: 835-842
  • NAID: 10020003952
  • Peer Reviewed
• [Journal Article] Efficient establishment of mouse embryonic stem cell lines from single blastomeres and polar bodies. (2007)
  • Author(s): Wakayama S.
  • Journal Title: Stem Cells. 25巻4号 Pages: 986-993
  • Peer Reviewed
• [Journal Article] Spindle formation and microtubule organization during first division in reconstructed rat embryos produced by somatic cell nuclear transfer. (2007)
  • Author(s): Tomioka I.
  • Journal Title: Journal of Reproduction and Development 53巻4号 Pages: 835-842
  • Peer Reviewed
• [Presentation] ライブセルイメージングによるマウス体細胞クローン胚の解析およびクローン産仔の作出 (2008)
  • Author(s): 水谷英二
  • Organizer: 第101 日本繁殖生物学会
  • Place of Presentation: 九州大学医学部百年講堂
  • Year and Date: 2008-09-20
• [Presentation] ライブセルイメージングによるマウス体細胞クローン胚の解析およびクローン産仔の作出 (2008)
  • Author(s): 水谷英二
  • Organizer: 第101日本繁殖生物学会
  • Place of Presentation: 九州大学医学部百年講堂
  • Year and Date: 2008-09-20
• [Presentation] Successfully producing cloned mice from somatic cells of aged mice via established ntES cell lines. (2008)
  • Author(s): 水谷英二
  • Organizer: 34^<th> International Embryo Transfer Society
  • Place of Presentation: Denver, Colorado, USA
• [Presentation] Retrospective and correlation analysis of the cloned embryos using long-term, six dimensional live-cell imaging (2008)
  • Author(s): 水谷英二
  • Organizer: 5^<th> Asian Reproductive Biotechnology Society
  • Place of Presentation: Kunming, Yunnan, China
• [Presentation] Retrospective and correlation analysis of the cloned embryos using lone-term, six dimensional live-cell imaging (2008)
  • Author(s): Mizutani E
  • Organizer: 5^<th> Asian Eeproductive Biotechnology Society
  • Place of Presentation: Kunming, Yunnan, China
• [Presentation] Successfully producing cloned mice from somatic cells of aged mice viaestablished ntES cell lines. (2008)
  • Author(s): Eiji Mizutani
  • Organizer: 34th International Embryo Transfer Society
  • Place of Presentation: Denver, Colorado, USA
• [Presentation] 高齢マウス体細胞からの胚性幹細胞の樹立と体細胞クローン個体作出 (2007)
  • Author(s): 水谷英二
  • Organizer: 第100回 日本繁殖生物学会
  • Place of Presentation: 東京大学農学部
  • Year and Date: 2007-10-20
• [Presentation] 高齢マウス体細胞からの胚性幹細胞の樹立と体細胞クローン個体作出 (2007)
  • Author(s): 水谷英二
  • Organizer: 第百回 日本繁殖生物学会
  • Place of Presentation: 東京大学農学部
  • Year and Date: 2007-10-20
• [Presentation] Successfully producing cloned mice from somatic cells of aged mice via established ntES cell lines. (2007)
  • Author(s): 水谷英二
  • Organizer: 4^<th> Asian Reproductive Biotechnology Society
  • Place of Presentation: Singapore
• [Presentation] Successfully producing cloned mice from somatic cells of aged mice viaestablished ntES cell lines. (2007)
  • Author(s): Eiji Mizutani
  • Organizer: 4th Asian Reproductive Biotechnology Society
  • Place of Presentation: Singapore