Molecular basis of herpesvirus transgenic mice as a new cerebellar hypoplasia model

• Project/Area Number: 19780222
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied veterinary science
• Research Institution: Hokkaido University
• Principal Investigator: TOMIOKA Yukiko Hokkaido University, 遺伝子病制御研究所, 助教 (50374674)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,490,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: トランスジェニックマウス / ヘルペスウイルス / 神経病理 / ヘルペスイルス / トランスジェニックマス / 小脳形成不全 / 神経病理学 / 獣医学

Research Abstract

仮性狂犬病ウイルス(PRV)は動物に重篤な神経症状を引き起こすため、獣医学領域で極めて重要なヘルペスウイルスである。PRV の転写調節因子IE180 を発現するトランスジェニックマウス (TgIE180)は小脳形成不全を呈し、IE180が小脳の発生に影響を与えることが示唆されている。本研究では、TgIE180の解析から、ウイルスの感染という現象を伴わずIE180が単独で神経細胞やグリア細胞に変性・細胞死を誘発することを明らかにし、IE180 の発現によりヘルペスウイルス感染の病態が一部再現されることを示唆した。また、IE180が細胞の恒常性維持に重要な蛋白質分解経路に関連する宿主分子に作用し、異常蛋白質の蓄積を促進する可能性を示した。

Research Products

• [Journal Article] Fusion protein consisting of the first immunoglobulin-like domain of porcine nectin-1 and Fc portion of human IgG1 provides a marked resistance against pseudorabies virus infection to transgenic mice (2009)
  • Author(s): Tomioka, Y., Morimatsu, M., Amagai, K., Kuramochi, M., Watanabe, Y., Kouda, S., Wada, T., Kuboki, N., Ono, E
  • Journal Title: Microbiol. Immunol 53 Pages: 8-15
  • NAID: 10026403895
  • Peer Reviewed
• [Journal Article] Fusion protein consisting of the first immunoglobulin-like domain of porcine nectin-1 and Fc portion of human IgG1 provides a marked resistance against pseudorabies virus infection to transgenic mice (2009)
  • Author(s): Tomioka. Y. et.al.
  • Journal Title: Microbiol. Immunol. 53 Pages: 8-15
  • NAID: 10026403895
  • Peer Reviewed
• [Journal Article] Cerebellar pathology in transgenic mice expressing the pseudorabies virus immediate-early protein IE180 (2008)
  • Author(s): Tomioka, Y., Miyazaki, T., Taharaguchi, S., Yoshino, S., Morimatsu, M., Uede,T., Ono, E., Watanabe, M
  • Journal Title: Eur. J. Neurosci 27 Pages: 2115-2132
  • Peer Reviewed
• [Journal Article] Microphthalmia and lack of vitreous body in transgenic mice expressing the first immunoglobulin-like domain of nectin-1 (2008)
  • Author(s): Yoshida, K., Tomioka, Y., Kase, S., Morimatsu, M., Shinya, K., Ohno, S., Ono,E
  • Journal Title: Graefes Arch. Clin. Exp. Ophthalmol 246 Pages: 543-549
  • Peer Reviewed
• [Journal Article] Cerebellar pathology in transgenic mice expressing the pseudorabies virus immediate-early protein IE180 (2008)
  • Author(s): Tomioka. Y. et.al.
  • Journal Title: Eur. J. Neurosci. 27 Pages: 2115-2132
  • Peer Reviewed
• [Journal Article] Microphthalmia and lack of vitreous body in transgenic mice expressing the first immunoglobulin-like domain of nectin-1 (2008)
  • Author(s): Yoshida. K. et.al.
  • Journal Title: Graefes Arch. Clin. Exp. Ophthalmol 246 Pages: 543-549
  • Peer Reviewed
• [Journal Article] Cerebellar pathology in transgenic mice expressing the pseudorabies virus immedi ate-early protein IE180 (2008)
  • Author(s): Tomioka. Y., et. al.
  • Journal Title: European Journal of Neuroscience 27 Pages: 2115-2132
  • Peer Reviewed
• [Journal Article] Possible roles of transcription factorsof pseudorabies virus in neuropathogenicity (2007)
  • Author(s): Ono, E., Tomioka, Y., Taharaguchi
  • Journal Title: Fukuoka Igaku Zasshi 98 Pages: 364-372
  • Peer Reviewed
• [Journal Article] Possible roles of transcription factors of pseudorabies virus in neuropathogenicity (2007)
  • Author(s): Ono. E., et. al.
  • Journal Title: Fukuoka Igaku Zasshi 98 Pages: 364-372
  • Peer Reviewed
• [Presentation] 仮性狂犬病ウイルス前初期蛋白IE180発現マウスにおける小脳形成不全の病理組織学的解析 (2008)
  • Author(s): 富岡幸子
  • Organizer: 第145回日本獣医学会学術集会
  • Place of Presentation: 麻布大学
  • Year and Date: 2008-03-28
• [Presentation] 仮性狂犬病ウイルスの転写調節因子IE180 の発現による神経病理発生メカニズムの解析 (2007)
  • Author(s): 富岡幸子
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] 仮性狂犬病ウイルスの転写調節因子IE180の発現による神経病理発生メカニズムの解析 (2007)
  • Author(s): 富岡 幸子
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14