Study on enhancement of Japanese encephalitis virus replication by the core protein

• Project/Area Number: 19780226
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied veterinary science
• Research Institution: Osaka University
• Principal Investigator: MORI Yoshio Osaka University, 微生物病研究所, 助教 (40379095)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,880,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: フラビウイルス / 日本脳炎ウイルス / C型肝炎ウイルス / コア蛋白質 / カテプシンL / シグナルペプチドペプチダーゼ / 日本脳炎アイルス / フラビアイルス

Research Abstract

日本脳炎ウイルスのコア蛋白質はウイルス粒子を形成する以外にも、ウイルスRNAの複製促進にも機能している。コア蛋白質はカテプシンL によって切断を受け、特定の細胞内での増殖性に関与していることが明らかとなった。また、同じフラビウイルス科ウイルスであるC 型肝炎ウイルスのコア蛋白質のシグナルペプチドペプチダーゼによる切断は、コア蛋白質の界面活性剤耐性膜局在およびウイルス粒子形成に必須であった。

Research Products

• [Journal Article] Intramembrane processing by signal peptide peptidase regulates the membrane localization of hepatitis C virus core protein and viral propagation. (2008)
  • Author(s): K. Okamoto*, Y. Mori*, Y. Komoda, T. Okamoto, M. Okochi, M. Takeda, T. Suzuki, K. Moriishi, and Y. Matsuura.(*These authors equally contributed to this work.)
  • Journal Title: J. Virol. 82 Pages: 8349-8361
  • Peer Reviewed
• [Journal Article] Hepatitis C virus core protein : Its coordinate roles with PA28gamma in metabolic abnormality and carcinogenicity in the liver (2008)
  • Author(s): Y. Mori, K. Moriishi, and Y. Matsuura.
  • Journal Title: Int. J. Biochem. Cell. Biol. 40 Pages: 1437-1442
  • Peer Reviewed
• [Journal Article] Crystal structure of the catalytic domain of Japanese encephalitis virus NS3 helicase/nucleoside triphosphatase at a resolution of 1.8Å. (2008)
  • Author(s): T. Yamashita, H. Unno, Y. Mori, H. Tani, K. Moriishi, A. Takamizawa, M. Agoh, T. Tsukihara, and Y. Matsuura.
  • Journal Title: Virology 373 Pages: 426-436
  • Peer Reviewed
• [Journal Article] Human butyrate-induced transcript 1 interacts with hepatitis C virus NS5A and regulates viral replication. (2008)
  • Author(s): S. Taguwa, T. Okamoto, T. Abe, Y. Mori, T. Suzuki, K. Moriishi, and Y. Matsuura.
  • Journal Title: J. Virol. 82 Pages: 2631-2641
  • Peer Reviewed
• [Journal Article] HCV複製に必須な宿主因子の同定と創薬の可能性 (2008)
  • Author(s): 森 嘉生、森石恆司、松浦善治
  • Journal Title: 細胞工学 27 Pages: 1226-1231
• [Journal Article] Intramembrane processing by signal peptide peptidase regulates the membrane localization of hepatitis C virus core protein and viral propagation. (2008)
  • Author(s): K. Okamoto
  • Journal Title: Journal of Virology 82(17) Pages: 8349-8361
  • Peer Reviewed
• [Journal Article] Hepatitis C virus core protein : its coordinate roles with PA28gamma in metabolic abnormality and carcinogenicity in the liver (2008)
  • Author(s): Y. Mori
  • Journal Title: The International Journal of Biochemistry and Cell Biology 40 Pages: 1437-1442
  • Peer Reviewed
• [Journal Article] Human butyrate-induced transcript 1 interacts with hepatitis C virus NS5A and regulates viral replication (2008)
  • Author(s): S. Taguwa
  • Journal Title: Journal of Virology 82(6) Pages: 2631-2641
  • Peer Reviewed
• [Journal Article] HCVの複製に必須な宿主因子の同定と創薬の可能性 (2008)
  • Author(s): 森嘉生
  • Journal Title: 細胞工学 27 Pages: 1226-1231
• [Journal Article] Processing of Capsid Protein by Cathepsin L Plays a Crucial Role in Replication of the Japanese Encephalitis Virus in Neural and Macrophage Cells. (2007)
  • Author(s): Y. Mori, T. Yamashita, Y. Tanaka, Y. Tsuda, T. Abe, K. Moriishi, and Y. Matsuura.
  • Journal Title: J. Virol. 82 Pages: 8477-8487
  • Peer Reviewed
• [Journal Article] Processing of capsid protein by cathepsin L plays a crucial role in replication of Japanese encephalitis virus in neural and macrophage cells (2007)
  • Author(s): Y. Mori, et. al.
  • Journal Title: Journal of Virology 81 Pages: 8477-8487
  • Peer Reviewed
• [Journal Article] Crystal structure of the catalytic domain of Japanese encephalitis virus NS3 helicase/nucleoside triphosphatase at a resolution of 1.8 A. (2007)
  • Author(s): T. Yamashita, et. al.
  • Journal Title: Virology (In press)
  • Peer Reviewed
• [Presentation] C型肝炎ウイルスコア蛋白質のシグナルペプチドペプチダーゼによるプロセッシングの生物学的意義 (2007)
  • Author(s): 森 嘉生, ほか
  • Organizer: 第55回日本ウイルス学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2007-10-22
• [Presentation] Intramembrane processing by SPP regulates membrane of HCV core protein and viral propagation. (2007)
  • Author(s): Y. Mori, et al.
  • Organizer: 14th International symposium on hepatitis C virus & related viruses.
  • Place of Presentation: Glasgow, UK
  • Year and Date: 2007-09-10
• [Presentation] Intramembrane processing by SPP regulates membrane of HCV core protein and viral propagation (2007)
  • Author(s): Y. Mori, et. al.
  • Organizer: 14th International symposium on hepatitis C virus & related viruses
  • Place of Presentation: Glasgow, UK
  • Year and Date: 2007-09-10
• [Book] 「タンパク質の事典」(猪飼篤・伏見譲・卜部格・上野川修一・中村春木・浜窪隆雄編) (2008)
  • Author(s): 森 嘉生、谷 英樹、松浦善治
  • Publisher: 朝倉書店
• [Book] タンパク質の事典ウイルス学(医学) (2008)
  • Author(s): 森嘉生
  • Publisher: 朝倉書店