Fabrication of functional bio-interface for immobilization of living cells by using plasma-irradiated polymer surface

• Project/Area Number: 19790038
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Physical pharmacy
• Research Institution: Gifu Pharmaceutical University
• Principal Investigator: SASAI Yasushi Gifu Pharmaceutical University, 薬学部, 講師 (60336633)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥2,500,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: プラズマ加工 / 表面・界面物性 / バイオインターフェイス / 細胞接着 / ペプチド / 細胞培養 / 原子移動ラジカル重合 / 細胞接着ペプチド / ペプチド固定化基板 / ポリスチレン

Research Abstract

弱電離気体を利用するプラズマ技術を基盤とした手法により、細胞培養器材に汎用されているポリスチレン(PS)基板表面へ細胞接着を制御するインターフェイスを設計および構築した。プラズマ技術を利用して化学的に不活性なPS表面にカルボキシル基を導入し、そのカルボキシル基を介して細胞接着性ペプチドの固定化することで、PS表面の細胞接着性を飛躍的に改善し、かつ細胞増殖を有為に促進させることができた。足場依存性細胞の生存や活動に接着は不可欠であり、本研究成果は培養細胞の有効利用に有益なものと考えられる。

Research Products

• [Journal Article] Introduction of Carboxyl Group onto Polystyrene Surface Using Plasma Techniques (2008)
  • Author(s): Yasushi Sasai, Natsuko Matsuzaki, Shin-ichi Kondo, Masayuki Kuzuya
  • Journal Title: Surface and Coatings Technology 202 Pages: 5724-5727
  • Peer Reviewed
• [Journal Article] Surface Engineering of Polystyrene Dish for Improvement of Cell Adhesion Using Plasma Techniques (2008)
  • Author(s): Yasushi Sasai, Natsuko Matsuzaki, Shin-ichi Kondo, Yukinori Yamauchi Masayuki Kuzuya
  • Journal Title: Journal of Photopolymer Science and Technology 21 Pages: 277-280
  • NAID: 40016108979
  • Peer Reviewed
• [Journal Article] Introduction of Carboxyl Group onto Polystyrene Surface Using Plasma Techniques (2008)
  • Author(s): 笹井泰志
  • Journal Title: Surface and Coatings Technology 202 Pages: 5724-5727
  • Peer Reviewed
• [Journal Article] Surface Engineering of Polystyrene Dish for Improvement of Cell Adhesion Using Plasma Techniques (2008)
  • Author(s): 笹井泰志
  • Journal Title: Journal of Photopolymer Science and Technology 21 Pages: 277-280
  • NAID: 40016108979
  • Peer Reviewed
• [Journal Article] Surface Engineering of Polymer Sheet by Plasma Techniques and Atom Transfer Radical Polymerization for Covalent Immobilization of Biomolecules (2007)
  • Author(s): Yasushi Sasai, Michinori Oikawa, Shin-ichi Kondo, Masayuki Kuzuya
  • Journal Title: Journal of Photopolymer Science and Technology 20 Pages: 197-200
  • NAID: 130004464526
  • Peer Reviewed
• [Journal Article] Surface engineering of polymer sheet by plasma techniques and atom transfer radical polymerization for covalent immobilization of biomolecules (2007)
  • Author(s): 笹井 泰志
  • Journal Title: Journal of photopolymer science and technology 20 Pages: 197-200
  • NAID: 130004464526
  • Peer Reviewed
• [Presentation] プラズマ照射高分子表面を利用したペプチド固定化基板の構築とその細胞培養への応用 (2009)
  • Author(s): 笹井泰志、松崎奈津子、近藤伸一、山内行玄、葛谷昌之
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-26
• [Presentation] Fabrication of Artificial Extracellular Matrix on Polymer Substrate Using Plasma Techniques (2009)
  • Author(s): Yasushi Sasai, Natsuko Matsuzaki, Shin-ichi Kondo, Yukinori Yamauchi, Masayuki Kuzuya
  • Organizer: 9th International Symposium on Biomimetic Materials Processing
  • Place of Presentation: 名古屋
  • Year and Date: 2009-01-21
• [Presentation] Fabrication of Artificial Extracellular Matrix on Polymer Substrate Using Plasma Techniques (2009)
  • Author(s): 笹井泰志
  • Organizer: 9^<th> International Symposium on Biomimetic Materials Processing
  • Place of Presentation: 名古屋
  • Year and Date: 2009-01-21
• [Presentation] プラズマ技術を利用したポリスチレン表面の機能化による細胞培養基板の構築 (2008)
  • Author(s): 松崎奈津子、笹井泰志、近藤伸一、葛谷昌之
  • Organizer: 日本薬学会東海支部例会
  • Place of Presentation: 静岡
  • Year and Date: 2008-12-06
• [Presentation] プラズマ技術を利用する細胞接着性改善を目的としたポリスチレンの表面設計 (2008)
  • Author(s): 笹井泰志、松崎奈津子、近藤伸一、山内行玄、葛谷昌之
  • Organizer: 第25回国際フォトポリマーコンファレンス
  • Place of Presentation: 千葉
  • Year and Date: 2008-06-26
• [Presentation] プラズマ技術と原子移動ラジカル重合によるポリエチレン表面の機能化と血液適合性付与 (2008)
  • Author(s): 笹井泰志、近藤伸一、山内行玄、葛谷昌之
  • Organizer: 第57回高分子学会年次大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-05-29
• [Presentation] プラズマ技術を利用する細胞の接着性改善を目的としたポリスチレン表面の機能化 (2008)
  • Author(s): 松崎奈津子、笹井泰志、近藤伸一、山内行玄、葛谷昌之
  • Organizer: 第57回高分子学会年次大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-05-29
• [Presentation] Introduction of carboxyl group onto polystyrene surface using plasma techniques, (2007)
  • Author(s): Yasushi Sasai, Natsuk Matsuzaki, Shin-ichi Kondo, Masayuki Kuzuya
  • Organizer: 6th Asian-European Inter- national Conference on Plasma Surface Engineering
  • Place of Presentation: 長崎
  • Year and Date: 2007-09-28
• [Presentation] Introduction of carboxyl group onto polystyrene surface using plasma techniques (2007)
  • Author(s): 笹井 泰志
  • Organizer: 6th Asian-European International Conference on Plasma Surface Engineering
  • Place of Presentation: 長崎(伊王島)
  • Year and Date: 2007-09-28
• [Presentation] Surface engineering of LDPE sheet by plasma techniques and atom transfer radical polymerization for fabrication of blood-compatible materials (2007)
  • Author(s): Yasushi Sasai, Michinori Oikawa, Shin-ichi Kondo, Masayuki Kuzuya
  • Organizer: 18th International Symposium on Plasma Chemistry
  • Place of Presentation: 京都
  • Year and Date: 2007-08-27
• [Presentation] Surface engineering of LDPE sheet by plasma techniques and atom transfer radical polymerization for fabrication of blood-compatible materials (2007)
  • Author(s): 笹井 泰志
  • Organizer: 18th International Symposium on Plasma Chemistry
  • Place of Presentation: 京都(京都大)
  • Year and Date: 2007-08-27
• [Presentation] プラズマ技術と原子移動ラジカル重合法による生体分子固定化用高分子表面の設計 (2007)
  • Author(s): 笹井泰志、及川倫徳、近藤伸一、葛谷昌之
  • Organizer: 第24回フォトポリマーコンファレンス
  • Place of Presentation: 千葉
  • Year and Date: 2007-06-26
• [Presentation] プラズマ技術と原子移動ラジカル重合法による生体分子固定化用高分子表面の設計 (2007)
  • Author(s): 笹井 泰志
  • Organizer: 第25回フォトポリマーコンファレンス シンポジウム:プラズマ光化学と高分子表面機能化
  • Place of Presentation: 千葉(千葉大)
  • Year and Date: 2007-06-26