Antigenic analysis of the food allergen using multi-dimensional HPLC system

• Project/Area Number: 19790044
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Physical pharmacy
• Research Institution: National Institute of Health Sciences
• Principal Investigator: SAKAI Shinobu National Institute of Health Sciences, 代謝生化学部, 主任研究官 (60370938)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,660,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥2,100,000 (Direct Cost: ¥2,100,000)
• Keywords: 食物アレルギー / 抗原解析 / 高速液体クロマトグラフィー / ウェスタンブロッティング / ウエスタンブロッティング

Research Abstract

食物アレルギーを誘発する成分は、食品を構成する多種多様な成分の中でもある特定のタンパク質であると推定される。本研究では、わが国における食物アレルギーの発症率及び重篤度の高いものの中で、主要抗原が塩不溶性のため、これまでに解析の研究が進んでいない小麦、大麦、ライ麦等の穀物中の主要抗原について2次元HPLCシステムを利用したプロファイリング分析を試みた。

Research Products

• [Journal Article] Interlaboratory Evaluation of Two Enzyme-Linked Immunosorbent Assay Kits for the Determination of Crustacean Protein in Processed Foods (2008)
  • Author(s): Shinobu SAKAI, Rieko MATSUDA, Reiko ADACHI, Hiroshi AKIYAMA, Tamio MAITANI, Yasuo OHNO, Michihiro OKA, Akihisa ABE, Kosuke SEIKI, Hiroshi ODA, Kazuo SHIOMI, Atsuo URISU
  • Journal Title: Journal of AOAC International 91 Pages: 123-129
  • Peer Reviewed
• [Journal Article] A Survey of CrustaceanSoluble Proteins such as "Shrimp" and "Crab" Content in the Food Ingredients (2008)
  • Author(s): Shinobu SAKAI, Reiko ADACHI, Yusuke SHIBAHARA, Michihiro OKA, Akihisa ABE, Kosuke SEIKI, Hiroshi ODA, Hisashi YOSHIOKA, Kazuo SHIOMI, Atsuo URISU, Hiroshi AKIYAMA, Reiko TESHIMA
  • Journal Title: Japanese Journal of Food Chemistry 15 Pages: 12-17
  • NAID: 110007367364
  • Peer Reviewed
• [Journal Article] Studies of the Nitrate Content in Vegetables and Processed Foods (2008)
  • Author(s): Shinobu SAKAI, Rieko MATSUDA, Toshiaki SUGIMOTO, Tamio MAITANI
  • Journal Title: Japanese Journal of Food Chemistry 15 Pages: 110-115
  • NAID: 110007367375
  • Peer Reviewed
• [Journal Article] Interlaboratory Evaluation of Two Enzyme-Linked Immunosorbent Assay Kits for the Determination of Crustacean Protein in Processed Foods (2008)
  • Author(s): Shinobu SAKAI
  • Journal Title: Journal of AOAC International 91 Pages: 123-129
  • Peer Reviewed
• [Journal Article] A Survey of Crustacean Soluble Proteins such as "Shrimp" and "Crab" Content in the Food Ingredients (2008)
  • Author(s): Shinobu SAKAI
  • Journal Title: Japanese Journal of Food Chemistry 15 Pages: 12-17
  • NAID: 110007367364
  • Peer Reviewed
• [Journal Article] Studies of the Nitrate Content in Vegetables and Processed Foods (2008)
  • Author(s): Shinobu SAKAI
  • Journal Title: Japanese Journal of Food Chemistry 15 Pages: 110-115
  • NAID: 110007367375
  • Peer Reviewed
• [Journal Article] Cross Reactivity of Allergen (2007)
  • Author(s): Hiroshi AKIYAMA, Shinobu SAKAI, Hiroki SAEKI, Kazuhiko WATANABE, Akira AKAZAWA, Atsuo URISU
  • Journal Title: Japanese Journal of Pediatric Medicine, 39 Pages: 558-563
• [Journal Article] Recent Trends and Future Prospects of Detection Method for Allergen in Japanese Allergy Labeling System (2007)
  • Author(s): Hiroshi AKIYAMA, Shinobu SAKAI, Reiko ADACHI
  • Journal Title: Food & Food Ingredients Journal of Japan 212 Pages: 1006-1015
• [Journal Article] Cross Reactivity of Allergen (2007)
  • Author(s): Hiroshi AKIYAMA
  • Journal Title: Japanese Joumal of Pediatric Medicine 39 Pages: 558-563
• [Journal Article] Recent Trends and Future Prospects of Detection Method for Allergens in Japanese Allergy Labeling System (2007)
  • Author(s): Hiroshi AKIYAMA
  • Journal Title: Foods & Food Ingredients Joumal of Japan 212 Pages: 1006-1015
• [Presentation] 2次元HPLCシステムを利用した食物アレルギー原因物質のプロファイリング分析 (2009)
  • Author(s): 酒井信夫, 穐山浩, 安達玲子, 手島玲子
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-26
• [Presentation] Interlaboratory Evaluation of Two Kinds of ELISA kits for the Determination of Soybean Protein and Walnut Protein in Processed Foods (2008)
  • Author(s): Shinobu SAKAI, Reiko ADACHI, Hiroshi AKIYAMA, Atsuo URISU, Reiko TESHIMA
  • Organizer: 122nd AOAC Annual Meeting
  • Place of Presentation: Dallas, USA
  • Year and Date: 2008-09-24
• [Presentation] Interlaboratory Evaluation of Two Kinds of ELISA Kits for the Determination of Soybean Protein and Walnut Protein in Processed Foods (2008)
  • Author(s): Shinobu SAKAI
  • Organizer: 122nd AOAC Annual Meeting
  • Place of Presentation: Dallas, TX
  • Year and Date: 2008-09-24
• [Presentation] 食品関連成分の機能と分析に関する研究 (2008)
  • Author(s): 酒井信夫
  • Organizer: 第14回日本食品化学学会
  • Place of Presentation: 兵庫
  • Year and Date: 2008-05-29
• [Presentation] Interlaboratory Evaluation of Two Kinds of ELISA kits for the Determinationof Crustacean Protein in Processed Foods (2007)
  • Author(s): Shinobu SAKAI, Rieko MATSUDA, Yuji SATO, Reiko ADACHI, Hiroshi AKIYAMA, Kazuo SHIOMI, Atsuo URISU, Tamio MAITANI, Yasuo OHNO
  • Organizer: 121st AOAC Annual Meeting
  • Place of Presentation: Anaheim, USA
  • Year and Date: 2007-09-18
• [Presentation] Novel ELISA System for the Detection of Walnut Protein in Processed Foods (2007)
  • Author(s): Hirotoshi DOI, Haruki SHIBATA, Masahiro SHOJI, Shinobu SAKAI, Hiroshi AKIYAMA
  • Organizer: 121st AOAC Annual Meeting
  • Place of Presentation: Anaheim, USA
  • Year and Date: 2007-09-18
• [Presentation] Interlaboratory Evaluation of Two Kinds of ELISA Kits for the Determination of Crustacean Protein in Processed Foods (2007)
  • Author(s): Shinobu SAKAI
  • Organizer: 121st AOAC Annual Meeting
  • Place of Presentation: Anaheim, CA
  • Year and Date: 2007-09-18
• [Presentation] Novel ELISA System for the Detection of Walnut Protein in Processed Foods (2007)
  • Author(s): Hirotoshi DOI
  • Organizer: 121st AOAC Annual Meeting
  • Place of Presentation: Anaheim, CA
  • Year and Date: 2007-09-18
• [Presentation] 水晶発振子(QCM)によるオボアルブミンの検出法について (2007)
  • Author(s): 鈴木友紀子, 水谷友海, 中村幹彦, 伊藤敦, 穐山浩, 酒井信夫, 近藤一成, 米谷民雄, 加藤重城, 秋元政信
  • Organizer: 第13回日本食品化学学会
  • Place of Presentation: 東京
  • Year and Date: 2007-06-01
• [Presentation] PCR法を用いた食品中のクルミの検知法について (2007)
  • Author(s): 酒井裕美子, 矢野竹男, 内山浩二, 中尾義喜, 石畑公江, 仲野茂, 山田敏広, 酒井信夫, 穐山浩, 米谷民雄
  • Organizer: 第13回日本食品化学学会
  • Place of Presentation: 東京
  • Year and Date: 2007-06-01
• [Presentation] PCR法を用い食品中のクルミの検知法について (2007)
  • Author(s): 酒井裕美子
  • Organizer: 第13回日本食品化学学会
  • Place of Presentation: 東京
  • Year and Date: 2007-06-01
• [Remarks] 日本薬学会第129年会ハイライト講演
  • URL: http://www.pharm.or.jp/hot-news/129kh/pdfs/073.pdf