Physical and functional interaction between WRNIP1 and RAD18

• Project/Area Number: 19790046
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Tohoku University
• Principal Investigator: YOSHIMURA Akari Tohoku University, 大学院・薬学研究科, 助手 (70302164)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,450,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: DNA修復 / WRNIP1 / RAD18 / ウェルナー症候群 / CPT

Research Abstract

本研究では、早老症ウェルナー症候群原因遺伝子産物WRNと相互作用するタンパク質として申請者の所属する研究室で見出されたWRNIP1の機能解析を目的とした。申請者は、損傷トレランス機構で働くRAD18とWRNIP1が相互作用することを初めて見出した。そこで、その機能的連携の解析から、WRNIP1が損傷トレランス機構の中心で働くRAD18と連携をとりながら、損傷トレランス機構に関与することが示唆された。

Research Products

• [Journal Article] Generation and characterization of cells that can be conditionally depleted of mitochondrial SOD2 (2009)
  • Author(s): Takada S, Inoue E, Tano K, Yoshii H, Abe T, Yoshimura A, Akita M, Tada S, Watanabe M, Seki M, Enomoto T
  • Journal Title: Biochem Biophys Res Commun 379 Pages: 233-8
  • Peer Reviewed
• [Journal Article] Physical and functional interaction between WRNIP1 and RAD18 (2009)
  • Author(s): Akari Yoshimura, et.al.
  • Journal Title: Genes Genet. Syst (印刷中)
  • NAID: 10024791852
  • Peer Reviewed
• [Journal Article] A novel role for Rad17 in homologous recombination (2008)
  • Author(s): Nishino K, Inoue E, Takada S, Abe T, Akita M, Yoshimura A, Tada S, Kobayashi M, Yamamoto K, Seki M, Enomoto T
  • Journal Title: Genes Genet Syst 83 Pages: 427-31
  • NAID: 10023888077
  • Peer Reviewed
• [Journal Article] KU70/80, DNA-PKcs, and Artemis are essential for the rapid induction of apoptosis after massive DSB formation (2008)
  • Author(s): Abe T, Ishiai M, Hosono Y, Yoshimura A, Tada S, Adachi N, Koyama H, Takata M, Takeda S, Enomoto T, Seki M.
  • Journal Title: Cell Signal 20 Pages: 1978-85
  • Peer Reviewed
• [Journal Article] Vertebrate WRNIP1 and BLM are required for efficient maintenance of genome stability (2008)
  • Author(s): Hayashi T, Seki M, Inoue E, Yoshimura A, Kusa Y, Tada S, Enomoto T
  • Journal Title: Genes Genet Syst 83 Pages: 95-100
  • NAID: 10024395708
  • Peer Reviewed
• [Journal Article] Analyses of functional interaction between RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase IIIalpha (2008)
  • Author(s): Otsuki M, Seki M, Inoue E, Abe T, Narita Y, Yoshimura A, Tada S, Ishii Y, Enomoto T
  • Journal Title: Biochim Biophys Acta 1782 Pages: 75-81
  • Peer Reviewed
• [Journal Article] A novel role for Rad17 in homologous recombination (2008)
  • Author(s): Katsuaki Nisnino, et.al.
  • Journal Title: Genes Genet. Syst 83 Pages: 233-238
  • Peer Reviewed
• [Journal Article] Generation and characterization of cells that can be conditionally depleted of mitochondrial SOD2 (2008)
  • Author(s): Syunya Takada, et.al.
  • Journal Title: Biochem. Biophys. Res. Commun 379 Pages: 233-238
  • Peer Reviewed
• [Journal Article] Ku70/80, DNA-PKcs, and Artemis, are essential for the rapid induction of apoptosis after massive DSB formation (2008)
  • Author(s): Takuya Abe, et.al.
  • Journal Title: Cell Signal 20 Pages: 1978-1985
  • Peer Reviewed
• [Journal Article] Vertebrate WRNIP1 and BLM are required for efficient maintenance of genome stability (2008)
  • Author(s): Hayashi T., et. al.
  • Journal Title: Genes Genet Syst. 83 Pages: 95-100
  • Peer Reviewed
• [Journal Article] Functional interactions between BLM and XRCC3 in the cell (2007)
  • Author(s): Otsuki M, Seki M, Inoue E, Yoshimura A, Kato G, Yamanouchi S, Kawabe Y, Tada S, Shinohara A, Komura J, Ono T, Takeda S, Ishii Y, Enomoto T
  • Journal Title: J Cell Biol 179(1) Pages: 53-63
  • Peer Reviewed
• [Journal Article] WRN functions in a RAD18-dependent damage avoidance pathway (2007)
  • Author(s): Dong YP, Seki M, Yoshimura A, Inoue E, Furukawa S, Tada S, Enomoto T
  • Journal Title: Biol Pharm Bull 30 Pages: 1080-3
  • NAID: 110006278395
  • Peer Reviewed
• [Journal Article] WRN counteracts the NHEJ pathway upon camptothecin exposure (2007)
  • Author(s): Otsuki M, Seki M, Kawabe Y, Inoue E, Dong YP, Abe T, Kato G, Yoshimura A, Tada S, Enomoto T
  • Journal Title: Biochem Biophys Res Commun 355 Pages: 477-82
  • Peer Reviewed
• [Journal Article] Analysis of functional interaction between RECQL1, RECQL5, and BLM which physically interact with DNA topoisomerase III alpha (2007)
  • Author(s): Otsuki M., et. al.
  • Journal Title: Biochim Biophys Acta 1782 Pages: 75-81
  • Peer Reviewed
• [Journal Article] Functional interaction between BLM and XRCC3 in the cell (2007)
  • Author(s): Otsuki M., et. al.
  • Journal Title: J Cell Biol. 179 Pages: 53-63
  • Peer Reviewed
• [Journal Article] WRN functions in a RAD 18-dependent damage avoidance pathway (2007)
  • Author(s): Dong YP, et. al.
  • Journal Title: Biol Pharm Bull. 30 Pages: 1080-1083
  • Peer Reviewed
• [Journal Article] Physical and functional interaction between WRNIP1 and RAD18
  • Author(s): Yoshimura A, Seki M, Kanamori M, Tateishi S, Tsurimoto T, Tada S, Enomoto T
  • Journal Title: Genes Genet Syst (in press)
  • NAID: 10024791852
  • Peer Reviewed
• [Presentation] WRNIP1のRAD180RAD6複合体への結合と複合体のDNA結合に及ぼす影響 (2009)
  • Author(s): 吉村明
  • Organizer: 日本薬学会 第129年会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-03-26
• [Presentation] WRNIP1のRAD18-RAD6複合体への結合と複合体のDNA結合に及ぼす影響 (2009)
  • Author(s): 吉村明
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 国立京都国際会館グランドプリンスホテル京都
  • Year and Date: 2009-03-26
• [Presentation] WRNIP1はRAD18-RAD6に結合し、RAD18-RAD6のもつ複製フォーク様構造への結合を阻害する (2008)
  • Author(s): 吉村明
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-11
• [Presentation] WRNIP1はRAD18-RAD6に結合し、RAD18-RAD6のもつ複製フォーク様構造への結合を阻害する (2008)
  • Author(s): 吉村明
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-11
• [Presentation] ニワトリDT40細胞を用いた抗酸化酵素遺伝子破壊株の解析 (2007)
  • Author(s): 井上 絵里, 他
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] WRNIP1とRAD18の機能的連携の解明 (2007)
  • Author(s): 吉村明
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-13
• [Presentation] 抗酸化酵素SOD2の転写on/off株の構築とその解析 (2007)
  • Author(s): 高田 隼也, 他
  • Organizer: 第46回日本薬学会東北支部大会
  • Place of Presentation: 東北薬科大学
  • Year and Date: 2007-10-28