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Complex formation and structural/functional analysis of chromatin-associated factors

Research Project

Project/Area Number 19790083
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

UMEHARA Takashi  The Institute of Physical and Chemical Research, システム研究チーム, 上級研究員 (20415095)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,550,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywords遺伝子発現 / 染色体 / クロマチン / ヒストン / 転写 / 構造解析
Research Abstract

染色体クロマチンの構造変換に関わる複合体の構造と機能を解明するため、タンパク質複合体の再構成に資する大腸菌内共発現ベクター系を構築した。また、ヒストン修飾酵素とヒストン、阻害剤を複合体化させ、ヒストン脱メチル化酵素LSD1の結晶構造を解明した。さらにヒストンシャペロンAsf1に対する2種類のシャペロン複合体を構造解明し、両結合因子がAsf1に対して類似した結合様式をもつことを見出した。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (9 results)

All 2008

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (6 results)

  • [Journal Article] Crystal structure of histone demethylase LSD1 and tranylcypromine at 2.25 A2008

    • Author(s)
      Mimasu, S., Sengoku, T., Fukuzawa, S., Umehara, T., Yokoyama, S
    • Journal Title

      Biochem. Biophys. Res. Commun 366

      Pages: 15-22

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structures of fission yeast histone chaperone Asf1 complexed with the Hip1 B-domain or the Cac2 C terminus2008

    • Author(s)
      Malay, A.D., Umehara, T., Matsubara- Malay, K., Padmanabhan, B., Yokoyama, S
    • Journal Title

      J. Biol. Chem 283

      Pages: 14022-14031

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structures of fission yeast histone chaperone Asf1 complexed with the Hipl B-domain or the Cac2 C terminus2008

    • Author(s)
      Malay A. D., et al.
    • Journal Title

      J. Biol. Chem. 283

      Pages: 14022-14031

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 複合体再構成のための大腸菌共発現ベクターの開発2008

    • Author(s)
      若森昌聡, 梅原崇史, 田仲昭子, 横山茂之
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-12
    • Related Report
      2008 Final Research Report
  • [Presentation] 複合体再構成のための大腸菌共発現ベクターの開発2008

    • Author(s)
      若森昌聡, 他
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] アセチル化ヒストンH4の認識を標的とするブロモドメイン阻害剤の開発2008

    • Author(s)
      梅原崇史, 伊藤 環, 中村祥浩, 佐々木和樹, 佐藤万仁, 寺田貴帆, 白水美香子, Padmanabhan B., 伊藤昭博, 田仲昭子, 吉田 稔, 横山茂之
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-11
    • Related Report
      2008 Final Research Report
  • [Presentation] Functional roles of the SWIRM domains in chromatin modification2008

    • Author(s)
      Umehara, T., Tochio, N., Mimasu, S., Yoneyama, M., Sengoku, T., Hayashizaki, Y., Ohara, O., Tanaka,A., Kigawa, T., Yokoyama, S
    • Organizer
      21st Naito Conference on Nuclear Dynamics and RNA
    • Place of Presentation
      山梨
    • Year and Date
      2008-06-24
    • Related Report
      2008 Final Research Report
  • [Presentation] Functional roles of the SWIRM domains in chromatin Modification2008

    • Author(s)
      梅原崇史, 他
    • Organizer
      21st Naito Conference on Nuclear Dynamics and RNA
    • Place of Presentation
      山梨県北杜市
    • Year and Date
      2008-06-24
    • Related Report
      2008 Annual Research Report
  • [Presentation] Structural study on the zinc-finger domains of Kruppel-like factors2008

    • Author(s)
      Nagashima, T., Izumi, K., Umehara, T., Hayashi, F., Shirouzu, M., Terada, T., Kigawa, T., Inoue, M., Yabuki, T., Aoki, M., Seki, E., Matsuda, T., Hirota, H., Yoshida, M., Tanaka, A., Suzuki, T., Nagai,R., Yokoyama, S
    • Organizer
      1st International Symposium on the Biology of the Kruppel-like Factors
    • Place of Presentation
      東京
    • Year and Date
      2008-03-06
    • Related Report
      2008 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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