Development of negative signal-inducing anti-allergic IgE

• Project/Area Number: 19790084
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: National Institute of Health Sciences
• Principal Investigator: NAKAMURA Ryosuke National Institute of Health Sciences, 代謝生化学部, 主任研究官 (50333357)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥570,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: 免疫化学 / 抗アレルギー薬 / IgE / マスト細胞 / シグナル伝達 / アレルギー試験

Research Abstract

Mast cells play important roles in the allergic reactions, and they express active signal-inducing high-affinity IgE receptors (FcεRI) and negative signal-inducing low-affinity IgG receptors (FcγRIIB) on their surface. In this study, new IgE molecule which may bind FcεRI and FcγRIIB simultaneously was genetically developed.

Research Products

• [Journal Article] A convenient and sensitive allergy test: IgE crosslinking-induced luciferase expression in cultured mast cells (2010)
  • Author(s): Nakamura R, Uchida Y, Higuchi M, Nakamura R, Tsuge I, Urisu A, Teshima R.
  • Journal Title: Allergy (in press)
  • Peer Reviewed
• [Journal Article] A convenient and sensitive allergy test : IgE crosslinking-induced luciferase expression in cultured mast cells. (2010)
  • Author(s): Nakamura R, Uchida Y, Higuchi M, Nakamura R, Tsuge I, Urisu A, Teshima R.
  • Journal Title: Allergy (In press (Epub))
  • Peer Reviewed
• [Journal Article] Allergenicity study of EGFP-transgenic chicken meat by serological and 2D-DIGE analysis. (2010)
  • Author(s): Nakamura R, Nakamura R, Nakano M, Arisawa K, Ezaki R, Horiuchi H, Teshima R.
  • Journal Title: Food and Chemical lbacicology 48 Pages: 1302-1310
  • Peer Reviewed
• [Journal Article] Major revision of the allergen database for food safety (ADFS) and validation of the motif-based allergenicity prediction tool. (2009)
  • Author(s): Nakamura R, Nakamura R, Teshima R.
  • Journal Title: Bulletin of National lnstitute of Health Sciences 127 Pages: 44-49
  • NAID: 40016925881
  • Peer Reviewed
• [Journal Article] Development of a novel allergy test using a cultured mast cell line (2009)
  • Author(s): Nakamura R, Uchida Y, Higuchi M, Teshima R.
  • Journal Title: ImmunoTox Letter 14 Pages: 12-13
• [Journal Article] Effects of developmental hypothyroidism induced by maternal administration of methimazole or propylthiouracil on the immune system of rats (2008)
  • Author(s): Nakamura, R., Teshima, R., Hachisuka, A., Sato, Y., Takagi, K., Nakamura, R., Woo, G. H., Shibutani, M., Sawada, J.
  • Journal Title: International Immunopharmacology 7 Pages: 1630-1638
  • Peer Reviewed
• [Journal Article] Effect of perinatal exposure to the flame-retardant tetrabromobisphenol A on the developing immune system of rats (2008)
  • Author(s): Nakamura, R., Hachisuka, A., Sato, Y., Nakamura, R., Shibutani, M., Sawada, J., Teshima, R.
  • Journal Title: Bulletin of National Institute of Health Sciences 126 Pages: 65-70
  • NAID: 40016369694
  • Peer Reviewed
• [Journal Article] Effect of exposure to decabromodiphenylether on the development of the immune system in rats (2008)
  • Author(s): Teshima, R., Nakamura, R., Nakamura, R., Hachisuka, A., Sawada, J., Shibutani, M.
  • Journal Title: Journal of Health Science 54 Pages: 382-389
  • Peer Reviewed
• [Journal Article] Effects of developmental hypothyroidism induced by maternal administration of methimazole or propylthiouracil on the immune system of rats (2007)
  • Author(s): Nakamura, R., Teshima, R., Hachisuka, A., Sato, Y., Takagi, K., Nakamura, R., Woo, G.H., Shibutani, M., Sawada, J.
  • Journal Title: International Immunopharmacology Vol.7、No.13 Pages: 1630-1638
  • Peer Reviewed
• [Journal Article] Effects of developmental hypothyroidism induced by maternal administration of methimazole or propylthiouracil on the immune system of rats. (2007)
  • Author(s): Nakamura R, Teshima R, Hachisuka A, Sato Y, Takagi K, Nakamura R, Woo GH, Shibutani M, Sawada J.
  • Journal Title: International Immunopharmacology 7 Pages: 1630-1638
  • Peer Reviewed
• [Presentation] EXiLF法-培養細胞を用いた新規アレルギー試験法の開発- (2010)
  • Author(s): 中村亮介、樋口雅一、内田好海、中村里香、手島玲子
  • Organizer: 第130回日本薬学会
  • Place of Presentation: 岡山県桃太郎アリーナ
  • Year and Date: 2010-03-30
• [Presentation] 遺伝子組み換え食品のアレルギー性評価法の確立 (2010)
  • Author(s): 中村里香、中村亮介、堀内浩幸、手島玲子
  • Organizer: 第130回日本薬学会
  • Place of Presentation: 岡山県桃太郎アリーナ
  • Year and Date: 2010-03-30
• [Presentation] EXiLE法-培養細胞を用いた新規アレルギー試験法の開発- (2010)
  • Author(s): 中村亮介、樋口雅一、内田好海、中村里香、手島玲子
  • Organizer: 第130回日本薬学会
  • Place of Presentation: 岡山市
• [Presentation] プロポリスの抗アレルギー作用 (2009)
  • Author(s): 中村里香、中村亮介、太田象三、加藤健司、三島敏、手島玲子
  • Organizer: 第82回日本生化学会大会合同年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-24
• [Presentation] 培養マスト細胞株の活性化に基づく新しい高感度アレルギー試験法の開発 (2009)
  • Author(s): 中村亮介、内田好海、樋口雅一、中村里香、手島玲子
  • Organizer: 第82回日本生化学会大会合同年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-23
• [Presentation] 遺伝子組み換え動物食品のアレルギー性評価法の検討 (2009)
  • Author(s): 中村里香、中村亮介、堀内浩幸、手島玲子
  • Organizer: 第16回日本免疫毒性学会学術大会
  • Place of Presentation: 旭川市民文化会館
  • Year and Date: 2009-08-28
• [Presentation] 培養細胞を用いた新しいアレルギー検査法の開発 (2009)
  • Author(s): 中村亮介、内田好海、樋口雅一、手島玲子
  • Organizer: 第16回日本免疫毒性学会学術大会
  • Place of Presentation: 旭川市民文化会館
  • Year and Date: 2009-08-27
• [Presentation] アレルゲンデータベースADFS (Allergen Database for Food Safety)のデータ改訂について (2009)
  • Author(s): 中村亮介, 中村里香, 手島玲子
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都国際会館
  • Year and Date: 2009-03-26
• [Presentation] 培養細胞を用いた新しいアレルギー検査法の開発 (2009)
  • Author(s): 中村亮介、内田好海、樋口雅一、手島玲子
  • Organizer: 第16回日本免疫毒性学会学術大会
  • Place of Presentation: 旭川市
• [Presentation] c-kit欠損W/W^vマウスにおけるタンパク質の経口感作とその関連遺伝子 (2008)
  • Author(s): 中村亮介, 酒井信夫, 松岡英樹, 秋山晴代, 佐藤雄嗣, 手島玲子
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-10
• [Presentation] カロテノイド摂取による抗原経口感作と食物アレルギー発症の抑制 (2008)
  • Author(s): 穐山浩, 佐藤雄嗣, 松岡英樹, 中村里香, 中村亮介, 石川信吾, 稲熊隆博, 戸塚護, 手島玲子
  • Organizer: 第15回日本免疫毒性学会学術大会
  • Place of Presentation: タワーホール船堀
  • Year and Date: 2008-09-12
• [Presentation] 臭素化難燃剤TBBPAの周産期暴露の仔ラット免疫系への影響 (2008)
  • Author(s): 中村亮介、手島玲子、蜂須賀暁子、佐藤雄嗣、中村里香、澤田純一、渋谷 淳
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-03-27
• [Presentation] キメラEGF受容体を用いるヒトIgE結合性の定量 (2007)
  • Author(s): 中村亮介、中村里香、手島玲子、澤田純一
  • Organizer: 第30回日本分子生物学会年会第80回日本生化学会大会合同年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
• [Presentation] Quantification of FcεRI-bindable human IgE using chimeric EGF receptor (2007)
  • Author(s): Nakamura R., Teshima R., Sawada J.
  • Organizer: 世界アレルギー学会2007
  • Place of Presentation: タイ、バンコク
  • Year and Date: 2007-12-05
• [Presentation] 母動物への臭素化難燃剤HBCDの周産期暴露の仔ラット免疫系への影響 (2007)
  • Author(s): 中村亮介、手島玲子、蜂須賀暁子、中村里香、澤田純一、渋谷 淳
  • Organizer: 第14回日本免疫毒性学会
  • Place of Presentation: 兵庫県民会館
  • Year and Date: 2007-09-20
• [Patent(Industrial Property Rights)] I型アレルギーの検査方法 (2009)
  • Inventor(s): 中村亮介、手島玲子
  • Industrial Property Rights Holder: 西島正弘
  • Industrial Property Number: 2009-168530
  • Filing Date: 2009-07-17