Identification and characterization of septin binding proteins

• Project/Area Number: 19790087
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Institute for Developmental Research, Aichi Human Service Center
• Principal Investigator: ITO Hidenori Institute for Developmental Research, Aichi Human Service Center, 神経制御学部, 主任研究員 (40311443)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,780,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: ニューロン / シナプス / シナプス小胞 / 開口放出 / dysbindin / 神経細胞 / スパイン / セプチン

Research Abstract

脳の発達に伴い増加するSept8に結合する分子の探索を行い、VAMP2およびdysbindin-1を同定した。Sept8とVAMP2の結合を解析し、Sept8は、神経伝達物質放出を制御している可能性があることを明らかにした。また、dysbindin-1の神経組織における解析を行い、dysbindin-1は、神経細胞の樹状突起形態を制御している可能性があることを明らかにした。

Research Products

• [Journal Article] Sept8 controls the binding of vesicle-associated membrane protein 2 to synaptophysin. (2009)
  • Author(s): Ito H, Atsuzawa K, Morishita R, Usuda N, Sudo K, Iwamoto I, Mizutani K, Katoh-Semba R, Nozawa Y, Asano T, Nagata K
  • Journal Title: J. Neurochem. 108 Pages: 867-880
• [Journal Article] Sept8 controls the binding of vesicle-associated membrane protein 2 to synaptophysin. (2009)
  • Author(s): Ito, H.
  • Journal Title: J. Neurochem. 108 Pages: 867-880
  • Peer Reviewed
• [Journal Article] Characterization of a multi-domain adaptor protein (2008)
  • Author(s): Ito H, Atsuzawa K, Sudo K, Di Stefano P, Iwamoto I, Morishita R, Takei S, Semba R, Defilippi P, Asano T, Usuda N, Nagata KI
  • Journal Title: J. Neurochem. 107 Pages: 61-72
• [Journal Article] Characterization of a multi-domain adaptor protein, p140Cap, as part of a presynaptic complex. (2008)
  • Author(s): Ito, H.
  • Journal Title: J. Neurochem. 107 Pages: 61-72
  • Peer Reviewed
• [Journal Article] Possible Roles of Vinexinβ in Growth and Paclitaxel Sensitivity in Human Prostate Cancer PC-3 Cells. (2007)
  • Author(s): Mizutani K, Nagata KI, Ito H, Ehara H, Nozawa Y, Deguchi T
  • Journal Title: Cancer Biol. Therapy 6 Pages: 1800-1804
• [Journal Article] Reversibility of the adverse effects of 1-bromopropane exposure in rats. (2007)
  • Author(s): Banu S, Ichihara S, Huang F, Ito H, Inaguma Y, Furuhashi K, Fukunaga Y, Wang Q, Kitoh J, Ando H, Kikkawa F, Ichihara G
  • Journal Title: Toxicol Sci. 100 Pages: 504-512
• [Journal Article] Essential roles of ERK-madiated phosphorylation of vinexin in cell spreading, migration and anchorage-independent growth (2007)
  • Author(s): Mizutani K, Ito H, Iwamoto I, Morishita R, Deguchi T, Nozawa Y, Asano T, Nagata KI
  • Journal Title: Oncogene. 26 Pages: 7122-7131
• [Journal Article] Involvement of Gq/11 in both integrin signal-dependent and -independent pathways regulating endothelin-induced neural progenitor proliferation (2007)
  • Author(s): Morishita R, Ueda H, Ito H, Tasaki J, Nagata KI, Asano T
  • Journal Title: Neurosci Res. 59 Pages: 205-214
• [Journal Article] Sept9 sequence alterations causing hereditary neuralgic amyotrophy are associated with altered interactions with Sept4/11 and resistance to Rho/Rhotekin-signaling. (2007)
  • Author(s): Sudo K, Ito H, Iwamoto I, Morishita R, Asano T, Nagata KI
  • Journal Title: Hum Mut. 28 Pages: 1005-1013
• [Journal Article] Localization of septin 8 in murine retina, and spatiotemporal expression of septin 8 in a murine model of photoreceptor cell degeneration. (2007)
  • Author(s): Hara A, Taguchi A, Niwa M, Aoki H, Yamada Y, Ito H, Nagata KI, Kunisada T, Mori H
  • Journal Title: Neurosci Lett. 423 Pages: 205-210
• [Journal Article] Expression of smooth muscle cell-specific proteins in neural progenitor cells induced by agonists of G protein-coupled receptors and transforming grwoth factor-beta (2007)
  • Author(s): Morishita R, Nagata KI, Ito H, Ueda H, Asano M, Shinohara H, Kato K, Asano T
  • Journal Title: J Neurochem. 101 Pages: 1031-1040
• [Journal Article] Expression of smooth muscle cell-specific proteins in neural progenitor cells induced by agonists of G protein-coupled receptors and transforming growth factor-beta (2007)
  • Author(s): Morisluta, R.
  • Journal Title: J.Neurochem 101 Pages: 1031-1040
  • Peer Reviewed
• [Journal Article] Localization of septin 8 in murine retina, and spatiotemporal expression of septin 8 in amurine model of photoreceptor cell degeneration (2007)
  • Author(s): Hara, A.
  • Journal Title: Neurosci.Lett. 423 Pages: 205-210
  • Peer Reviewed
• [Journal Article] Sept9 sequence alterations causing hereditary neuralgic amyotrophy are associated with altered interactions with Sept4/11 and resistance to Rho/Rhoteldn-signaling (2007)
  • Author(s): Sudo, K.
  • Journal Title: Hum. Mut 28 Pages: 1005-1013
  • Peer Reviewed
• [Journal Article] Involvement of Gq/11 in both integrin signal-dependent and-independent pathways regulating endothelin-induced neural progenitor proliferation (2007)
  • Author(s): Morislrita, R.
  • Journal Title: Neurosci. Res. 59 Pages: 205-214
  • Peer Reviewed
• [Journal Article] Essential roles of ERK-mediated phosphorylation of vinexin in cell spreading, migration and anchorage-independent growth (2007)
  • Author(s): Mizutani, K.
  • Journal Title: Oncogene 26 Pages: 7122-7131
  • Peer Reviewed
• [Journal Article] Reversibility of the adverse effects of 1-bromopropane exposure in rats. (2007)
  • Author(s): Banu, N.
  • Journal Title: Toxicol.Sci. 100 Pages: 504-512
  • Peer Reviewed
• [Presentation] Biochemical and histological analyses of dysbindin-1 in rat brain (2008)
  • Author(s): 伊東秀記, 森下理香, 須藤香織, 岩本郁子, 篠田友靖, 岡本賢一, 永田浩一
  • Organizer: 米国細胞生物学会
  • Place of Presentation: サンフランシスコ
  • Year and Date: 2008-12-16
• [Presentation] Possible role in neurotransmitter release and signaling of a multi-domain adaptor protein (2008)
  • Author(s): 永田浩一, 伊東秀記, 厚沢季美江, 須藤香織, Paola Di Stefano, 岩本郁子, Paola Defilippi, 森下理香, 武井史郎, 仙波禮治, 浅野富子, 臼田信光
  • Organizer: 米国細胞生物学会
  • Place of Presentation: サンフランシスコ
  • Year and Date: 2008-12-16
• [Presentation] Biochemical and histological analyses of dysbindin-1 in rat brain. (2008)
  • Author(s): 伊東秀記
  • Organizer: 米国細胞生物学会
  • Place of Presentation: サンフランシスコ
  • Year and Date: 2008-12-16
• [Presentation] Possible role in neurotransmitter release and signaling of a multi-domain adaptor protein, p140Cap, at the presynapse. (2008)
  • Author(s): 永田浩一
  • Organizer: 米国細胞生物学会
  • Place of Presentation: サンフランシスコ
  • Year and Date: 2008-12-16
• [Presentation] Biochemical and cell biological analysis of dysbindin-1 in rat brain. (2008)
  • Author(s): 伊東秀記, 森下理香, 須藤香織, 岩本郁子, 篠田友靖, 岡本賢一, 永田浩一
  • Organizer: 日本分子生物学会年会・日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] Possible function of a multi-domain adaptor protein, p140Cap (2008)
  • Author(s): 篠田友靖, 伊東秀記, 梶田和男, 須藤香織, 岩本郁子, 森下理香, 岡本賢一, 永田浩一
  • Organizer: 日本分子生物学会年会・日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] Biochemical and cell biological analysis of dysbindin-1 in rat brain. (2008)
  • Author(s): 伊東秀記
  • Organizer: 日本分子生物学会年会,日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] Possible function of a multi-domain adaptor protein, p140Cap, in exo-cytosis. (2008)
  • Author(s): 篠田友靖
  • Organizer: 日本分子生物学会年会,日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] Sept14の皮質神経細胞局在化における機能 (2008)
  • Author(s): 篠田友靖, 伊東秀記, 須藤香織, 岡本賢一, 岩本郁子, 森下理香, 永田浩一
  • Organizer: 日本神経化学会大会
  • Place of Presentation: 富山
  • Year and Date: 2008-09-12
• [Presentation] マルチドメイン・アダプター蛋白質p140Capのプレシナプスにおける機能 (2008)
  • Author(s): 永田浩一, 伊東秀記, 武井史郎, 須藤香織, 岩本郁子, 森下理香, 浅野富子, 仙波禮治
  • Organizer: 日本神経化学会大会
  • Place of Presentation: 富山
  • Year and Date: 2008-09-12
• [Presentation] Sept14の皮質神経細胞局在化における機能. (2008)
  • Author(s): 篠田友靖
  • Organizer: 日本神経化学会大会
  • Place of Presentation: 富山
  • Year and Date: 2008-09-12
• [Presentation] マルチドメイン・アダプター蛋白質p140Capのプレシナプスにおける機能. (2008)
  • Author(s): 永田浩一
  • Organizer: 日本神経化学会大会
  • Place of Presentation: 富山
  • Year and Date: 2008-09-12
• [Presentation] Biochemical and histological analysis of MAGI-1 in rat brain. (2008)
  • Author(s): 伊東秀記, 森下理香, 須藤香織, 岩本郁子, 篠田友靖, 岡本賢一, 永田浩一
  • Organizer: 日本神経化学会大会
  • Place of Presentation: 富山
  • Year and Date: 2008-09-11
• [Presentation] Biochemical and histological analysis MAGI-1 in rat brain. (2008)
  • Author(s): 伊東秀記
  • Organizer: 日本神経化学会大会
  • Place of Presentation: 富山
  • Year and Date: 2008-09-11
• [Presentation] マルチドメイン・アダプター蛋白質p140Capのプレシナプスにおける性状解析 (2008)
  • Author(s): 伊東秀記, 武井史郎, 須藤香織, 岩本郁子, 森下理香, 浅野富子, 仙波禮治, 永田浩一
  • Organizer: 日本生化学会中部支部会例会
  • Place of Presentation: 岐阜
  • Year and Date: 2008-05-24
• [Presentation] マルチドメイン・アダプター蛋白質p140Capのシナプスにおける性状解析. (2008)
  • Author(s): 伊東秀記
  • Organizer: 日本生化学会中部支部会例会
  • Place of Presentation: 岐阜
  • Year and Date: 2008-05-24
• [Presentation] Rhotekiタンパク質Lin-7Bの性状解析 (2007)
  • Author(s): 須藤香織, 伊東秀記, 岩本郁子, 森下理香, 永田浩一
  • Organizer: 日本生化学大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] Rhotekin結合タンパク質Lin-7Bの性状解析 (2007)
  • Author(s): 須藤 香織
  • Organizer: 第80回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] ERK-MAPキナーゼによるビネキシンのリン酸化は細胞接着・運動および足場非依存的な細胞増殖に必須の役割を果たす (2007)
  • Author(s): 永田浩一, 水谷晃輔, 伊東秀記, 岩本郁子, 森下理香, 出口隆, 野澤義則, 浅野富子
  • Organizer: 日本生化学大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Presentation] ERK-MAPキナーゼによるビネキシンのリン酸化は細胞接着・運動および足場非依存的な細胞増殖に必須の役割を果たす (2007)
  • Author(s): 永田 浩一
  • Organizer: 第80回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Presentation] Sept8によるシナプトブレビン/シナプトフィシン複合体形成の抑制 (2007)
  • Author(s): 伊東秀記, 森下理香, 岩本郁子, 須藤香織, 浅野富子, 永田浩一
  • Organizer: 日本生化学大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Sept8によるシナプトブレビン/シナプトフィシン複合体形成の抑制 (2007)
  • Author(s): 伊東 秀記
  • Organizer: 第80回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] Essential roles of ERK-mediated phosphorylation of vinexin in cell spreading, migration and anchorage-independet growth. (2007)
  • Author(s): 永田浩一, 水谷晃輔, 伊東秀記, 岩本郁子, 森下理香, 出口隆, 野澤義則, 浅野富子
  • Organizer: 米国細胞生物学会
  • Place of Presentation: ワシントン
  • Year and Date: 2007-12-03
• [Presentation] Essential Roles of ERK-mediated Phosphorylation of Vinexin in Cell Spreading, Migration, and Anchorage-independent Growth (2007)
  • Author(s): Nagata, K.
  • Organizer: 第47回米国細胞生物学会
  • Place of Presentation: Washington DC. USA
  • Year and Date: 2007-12-03
• [Presentation] 脳において生後発達期に増加するSept8とVAMP2の結合の解析 (2007)
  • Author(s): 伊東秀記, 岩本郁子, 須藤香織, 森下理香, 浅野富子, 永田浩一
  • Organizer: 日本神経学・神経化学・神経回路学合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-11
• [Presentation] 細胞骨格関連分子Septin12の生化学・細胞生物学的性状解析 (2007)
  • Author(s): 永田浩一, 伊東秀記, 須藤香織, 岩本郁子, 森下理香, 浅野富子
  • Organizer: 日本神経学・神経化学・神経回路学合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-11
• [Presentation] 遺伝性神経痛性筋萎縮症を引き起こすSept9の点変異はSept4とSept11の相互作用を変化させ、Rho/Rhotekinシグナル伝達を妨害する. (2007)
  • Author(s): 須藤香織, 伊東秀記, 岩本郁子, 森下理香, 浅野富子, 永田浩一
  • Organizer: 日本神経学・神経化学・神経回路学合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-11
• [Presentation] 細胞骨格関連分子Septinl2の生化学・細胞生物学的性状解析 (2007)
  • Author(s): 永田 浩一
  • Organizer: 第50回日本神経化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-11
• [Presentation] 脳において生後発達期に増加するSept8とVAMP2の結合の解析 (2007)
  • Author(s): 伊東 秀記
  • Organizer: 第50回日本神経化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-11
• [Presentation] 遺伝性神経痛性筋萎縮症を引き起こすSept9の点変異はSept4とSept11の相互作用を変化させRho/Rhotekinシグナル伝達を妨害する (2007)
  • Author(s): 須藤 香織
  • Organizer: 第50回日本神経化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-11
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