Mechanism of ribosome splitting

• Project/Area Number: 19790088
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: HIROKAWA Go National Cardiovascular Center Research Institute, 疫学部, 室長 (90450890)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,550,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: 蛋白質生合成

Research Abstract

リボソームはmRNAコドンの解読が行われる小サブユニットとペプチド結合の形成が行われる大サブユニットからなり、遺伝子の翻訳は両サブユニットがmRNA上で会合することで開始され、翻訳を終えたリボソームは再び各サブユニットへ解離して次の翻訳サイクルへとリサイクルされる。この反応は3因子とGTPの加水分解を必要とする反応であるが、GTPエネルギーの役割及びこの反応の順序について明らかにした。

Research Products

• [Journal Article] The role of GTP in transient splitting of 70S ribosomes by RRF (ribosome recycling factor) and EF-G (elongation factor G). (2008)
  • Author(s): Hirokawa G, Iwakura N, Kaji A, Kaji H
  • Journal Title: Nucleic Acids Res 36 Pages: 6676-6687
  • Peer Reviewed
• [Journal Article] Targeted deletion of miR-182, an abundant retinal microRNA. (2008)
  • Author(s): Jin ZB, Hirokawa G, Gui L, Takahashi R, Osakada F, Hiura Y, Takahashi M, Yasuhara O, Iwai N
  • Journal Title: Mol. Vis 15 Pages: 523-533
  • Peer Reviewed
• [Journal Article] Insights into ribosome recycling factor interactions with the 70S ribosome. (2008)
  • Author(s): Pai RD, Zhang W, Schuwirth BS, Hirokawa G, Kaji H, Kaji A, Cate JH., Structural
  • Journal Title: J Mol Biol 376 Pages: 1334-1347
  • Peer Reviewed
• [Journal Article] The role of GTP in transient splitting of 70S ribosomes by RRF (ribosome recycling factor) and EF-G (elongation factor G) (2008)
  • Author(s): Hirokawa G., et al.
  • Journal Title: Nucleic Acids Res. 36 Pages: 6676-6687
  • Peer Reviewed
• [Journal Article] Targeted deletion of miR-182, an abundant retinal micro RNA. (2008)
  • Author(s): Jin ZB., et al.
  • Journal Title: Mol. Vis. 15 Pages: 523-533
  • Peer Reviewed
• [Journal Article] Structural Insights into ribosome recycling factor interactions with the 70S ribos ome. (2008)
  • Author(s): Pai RD, et. al.
  • Journal Title: J Mol Biol. 376 Pages: 1334-1347
  • Peer Reviewed
• [Journal Article] Novel activity of eukaryotic trasnlocase, eEF2 : dissociation of the 80S ribosome into subunits with ATP but not with GTP. (2007)
  • Author(s): Demeshkina N, Hirokawa G, Kaji A, Kaji H
  • Journal Title: Nucleic Acids Res 35 Pages: 4597-4607
  • Peer Reviewed
• [Journal Article] Structural basis for aminoglycoside inhibition of bacterial ribosome recycling. (2007)
  • Author(s): Borovinskaya MA, Pai RD, Zhang W, Schuwirth BS, Holton JM, Hirokawa G, Kaji H, Kaji A, Cate JH
  • Journal Title: Nature Struct Mol Biol 14 Pages: 727-732
  • Peer Reviewed
• [Journal Article] Minor contribution of an internal ribosome entry site in the 5'-UTR of ornithine decarboxylase mRNA on its translation. (2007)
  • Author(s): Nishimura K, Sakuma A, Yamashita T, Hirokawa G, Imataka H, Kashiwagi K, Igarashi K
  • Journal Title: Biochem Biophys Res Commun 364 Pages: 124-130
  • Peer Reviewed
• [Journal Article] Novel activity of eukaryotic translocase,eEF2: dissociation of the 80S ribosome into subunits with ATP but not with GTP (2007)
  • Author(s): Demeshkina N., et. al.
  • Journal Title: Nucleic Acids Res. 35 Pages: 4597-4607
  • Peer Reviewed
• [Journal Article] Structural basis for aminoglycoside inhibition of bacterial ribosome recycling. (2007)
  • Author(s): Borovinskaya MA, et. al.
  • Journal Title: Nat Struct Mol Biol. 14 Pages: 727-732
  • Peer Reviewed
• [Journal Article] Minor contribution of an internal ribosome entry site in the 5'-UTR of ornithine decarboxylase mRNA on its translation (2007)
  • Author(s): Nishimura K, et. al.
  • Journal Title: Biochem Biophys Res Commun 364 Pages: 124-130
  • Peer Reviewed
• [Presentation] Serum microRNAs are promising biomarkers for several caridiovascular diseases (2009)
  • Author(s): Ji X, Takahashi R, Hirokawa G, Hiura Y, Iwai N
  • Organizer: 第73回日本循環器学会
  • Place of Presentation: 大阪国際会議場
• [Presentation] Validation of computer predicted target genes of mammalian microRNA-182 (2008)
  • Author(s): Le Gui, 廣川 剛, 福島康江, 岩井直温
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸国際会議場
• [Presentation] Is the expression profile of mRNA correlated with that of miRNA during adipocyte differentication ? (2007)
  • Author(s): Le Gui, 廣川 剛, 岩井直温
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: パシフィコ横浜