Elucidation of mechanism for antimetastatic action by methylcordycepin, a novel biological active compound, and its treatment strategy

• Project/Area Number: 19790101
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Drug development chemistry
• Research Institution: Mukogawa Women's University
• Principal Investigator: YOSHIKAWA Noriko Mukogawa Women's University, 薬学部, 助手 (40373120)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥2,900,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: メチルコーディセピン / アデノシンデアミナーゼ / アデノシンキナーゼ / ヒト線維肉腫細胞 / がん転移

Research Abstract

古来より中国において滋養強壮作用を有する生薬として使用されてきた冬虫夏草の有効成分であるコーディセピンをリード化合物として合成したメチルコーディセピンは、コーディセピンよりも持続的な抗がん活性を示した。その作用機序としてアデノシンキナーゼの活性化を介している可能性が示唆された。また、種々のがん細胞(マウスメラノーマ細胞、マウス肺がん細胞、ヒト大腸がん細胞およびヒト白血病細胞)に対して有用であったが、特に、転移能の高いヒト線維肉腫細胞に対して強い抗がん活性を示し、in vivoでヒト線維肉腫細胞に対する影響をマウスの生存日数を指標に検討したところ、メチルコーディセピンを投与したマウスはコントロールマウスと比較して有意に生存日数が延長した。

Research Products

• [Journal Article] Cordycepin (3'-deoxyadenosine) inhibits the growth of B16-BL6 mouse melanoma cells through the stimulation of adenosine A3 receptor followed by glycogen synthase kinase-3β activation and cyclin D1 suppression (2008)
  • Author(s): Noriko Yoshikawa,
  • Journal Title: Naunyn Schmiedebergs Arch. Pharmacol 377 Pages: 591-595
  • Peer Reviewed
• [Journal Article] Effect of P2 receptor on the intracellular calcium increase by cancer cells in human umbilical vein endothelial cells (2008)
  • Author(s): Namie Nejime, Noriko Yoshikawa
  • Journal Title: Naunyn Schmiedebergs Arch. Pharmaco 377 Pages: 429-436
  • Peer Reviewed
• [Journal Article] Effect of P2 receptor on the intracellular calcium increase by cancer cells in human umbilical vein endothelial cells (2008)
  • Author(s): Namie Nejime, Noriko Yoshikawa
  • Journal Title: Naunyn Schmiedebergs Arch. Pharmaco1. (in press)
  • Peer Reviewed
• [Journal Article] Cordycepin and Cordyceps sinensis reduce the growth of human promyelocytic leukemia cells through the Wnt signaling pathway (2007)
  • Author(s): Noriko Yoshikawa
  • Journal Title: Clin. Exp. Pharmacol. Physiol. 34 Pages: 61-63
  • Peer Reviewed
• [Journal Article] Cordycepin(3'-deoxyadenosine)inhibits the growth of B16-BL6 mouse melanoma cells through the stimulation of adenosine A3 receptor followed by glycogen synthase kinase-3 β activation and cyclin D1 suppression (2007)
  • Author(s): Noriko Yoshikawa
  • Journal Title: Naunyn Schmiedebergs Arch. Pharmacol. (in press)
  • Peer Reviewed
• [Presentation] 肝細胞増殖因子(HGF)により増強されたがん細胞浸潤能に対する冬虫夏草水抽出物の影響 (2009)
  • Author(s): 久保えり香
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-03-18
• [Presentation] ヒドロキシウレアは冬虫夏草水抽出物のがん転移抑制作用を増強する (2009)
  • Author(s): 吉川紀子
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-03-16
• [Presentation] 肝転移モデルマウスに対する冬虫夏草水抽出物のがん転移抑制効果 (2008)
  • Author(s): 久保えり香
  • Organizer: 第114回日本薬理学会近畿部会
  • Place of Presentation: 神戸
  • Year and Date: 2008-11-14
• [Presentation] Effect of Cordyceps sinensis on spontaneous metastatic model mice (2008)
  • Author(s): Noriko Yoshikawa
  • Organizer: 8th International Conference of Anticancer Research
  • Place of Presentation: Kos, Greece
  • Year and Date: 2008-10-18
• [Presentation] Effect of Cordyceps sinensis on spontaneous metastatic model mice (2008)
  • Author(s): Noriko Yoshikawa
  • Organizer: 8^<th> International Conference of Anticancer Research
  • Place of Presentation: Kos, Greece
  • Year and Date: 2008-10-18
• [Presentation] 冬虫夏草水抽出物の血小板凝集抑制を介するがん転移抑制作用 (2008)
  • Author(s): 吉川紀子
  • Organizer: 第113回日本薬理学会近畿部会,
  • Place of Presentation: 岡山
  • Year and Date: 2008-06-20
• [Presentation] 冬虫夏草水抽出物の血小板凝集抑制を介するがん転移抑制作用 (2008)
  • Author(s): 吉川紀子
  • Organizer: 第113回日本薬理学会近畿部会
  • Place of Presentation: 岡山
  • Year and Date: 2008-06-20
• [Presentation] 癌細胞による血管内皮細胞内カルシウム上昇にP2Y受容体が関与する (2008)
  • Author(s): 禰占奈美江
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-19
• [Presentation] 4種のマウスメラノーマ細胞における転移先臓器指向性の違いについて (2008)
  • Author(s): 久保えり香
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-17
• [Presentation] Cancer cells effect on intracellular calcium ion levels of vascular endothelial cells via P2Y receptors (2007)
  • Author(s): Namie Nejime
  • Organizer: 5th International Receptor Symposium
  • Place of Presentation: Shizuoka, Japan
  • Year and Date: 2007-05-10
• [Presentation] Cordycepin (3'-deoxyadenosine) reduces the growth of B16-BL6 mouse melanoma cells through the adenosine A3 receptor followed by Wnt signaling pathway (2007)
  • Author(s): Kazuki Nakamura
  • Organizer: 5th International Receptor Symposium
  • Place of Presentation: Shizuoka, Japan
  • Year and Date: 2007-05-10
• [Presentation] Cordycepin(3'-deoxyadenosine)reduces the growth of B16-BL6 mouse melanoma cells through the adenosine A_3 receptor followed by Wnt signaling pathway (2007)
  • Author(s): Kazuki Nakamura, Noriko Yoshikawa
  • Organizer: 5th International Receptor Symposium
  • Place of Presentation: Shizuoka, Japan
  • Year and Date: 2007-05-10
• [Remarks]
  • URL: http://ph.mukogawa-u.ac.jp/~yakuri1/achievements.htm
• [Remarks]
  • URL: http://ph.mukogawa-u.ac.jp/~yakuri1/achievements.htm
• [Remarks]
  • URL: http://ph.mukogawa-u.ac.jp/~yakuri1/achievements.htm