In silico and HTS screenings of oligosaccharide processing enzyme as drug discovery target

• Project/Area Number: 19790102
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Drug development chemistry
• Research Institution: Nihon University
• Principal Investigator: HAKAMATA Wataru Nihon University, 生物資源科学部, 講師 (10333337)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,860,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥660,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,000,000 (Direct Cost: ¥1,000,000)
• Keywords: 糖鎖 / ウイルス / 酵素反応 / 小胞体 / 翻訳後修飾 / in silico / スクリーニング / 翻訳後修

Research Abstract

For the development of a true alpha-inhibitor that acts in cells, in silico HTS using the target alpha-glucosidase and computer-aided inhibitors for screening based on enzymological information of alpha-glucosidase are important.

Research Products

• [Journal Article] Design and Screening Strategies forα-Glucosidase Inhibitor Based on Enzymological Information. (2009)
  • Author(s): Wataru Hakamata, Masaaki Kurihara, Haruhiro Okuda, Toshiyuki Nishio, Tadatake Oku
  • Journal Title: Current Topics in Medicinal Chemistry 9 Pages: 3-12
  • Peer Reviewed
• [Journal Article] Design and Screening Strategies for a-Glucosidase Inhibitor Based on Enzymological Information (2009)
  • Author(s): Wataru Hakamata, Masaaki Kurihara, Haruhiro Okuda, Toshiyuki Nishio, Tadatake Oku
  • Journal Title: Current Topics in Medicinal Chemistry 9 Pages: 3-12
  • Peer Reviewed
• [Presentation] In silico Screening of oligosaccharide processing glycosidase inhibitor for HIV drug (2009)
  • Author(s): Wataru Hakamata, Yutaka Takebe, Yoriko Ushijima, Toshiyui Nishio, Tadatake Oku
  • Organizer: 薬理学会第82回日本薬理学会年会
  • Place of Presentation: 神奈川県横浜市(シンポジウム招待講演)
  • Year and Date: 2009-03-18
• [Presentation] In silico Screening forα-Glucosidase Inhibitors (2008)
  • Author(s): W. Hakamata, Y. Ushijima, M. Kurihara, H. Okuda, T. Nishio, T. Oku
  • Organizer: XXIV International Carbohydrate Symposium
  • Place of Presentation: Oslo, Norway
  • Year and Date: 2008-07-30
• [Presentation] In silico Screening for α-Glucosidase Inhibitors (2008)
  • Author(s): W. Hakamata, Y. Ushijima, M. Kurihara, H. Okuda, T. Nishio, T. Oku
  • Organizer: International Carbohydrate Symposium
  • Place of Presentation: ノルウェー王国、オスロ
  • Year and Date: 2008-07-26
• [Presentation] 放線菌共培養ライブラリからのα-グルコシダーゼ阻害剤のスクリーニング (2008)
  • Author(s): 牛島世里子、寺島彩、袴田航、西尾俊幸、奥忠武
  • Organizer: 2008年日本農芸化学会
  • Place of Presentation: 京都
• [Presentation] グルコシダーゼ阻害剤生合成鍵酵素の存在を指標とした放線菌共培養液からの新規骨格を有するグルコシダーゼ阻害剤の探索 (2008)
  • Author(s): 牛島世里子, 袴田航, 西尾俊幸, 奥忠武
  • Organizer: 第57回日本応用糖質科学会
  • Place of Presentation: 琉球大学(沖縄県那覇市)
• [Presentation] α-グルコシダーゼ阻害剤のin silicoスクリーニングに用いる合成可能なバーチャル化合物ライブラリの構築 (2007)
  • Author(s): 袴田航、牛島世里子、寺島彩、栗原正明、奥田晴宏、西尾俊幸、奥忠武
  • Organizer: 第56回日本応用糖質科学会
  • Place of Presentation: 日本大学(神奈川県藤沢市)
• [Presentation] α-グルコシダーゼ阻害剤のin silicoスクリーニングに用いる合成可能なバーチャル化合物ライブラリの構築 (2007)
  • Author(s): 袴田 航
  • Organizer: 第56回日本応用糖質科学会
  • Place of Presentation: 日本大学 湘南キャンパス