Organ specific effector mechanisms induced by tumor antigen-specific DNA vaccination

• Project/Area Number: 19790118
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Medical pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: DENDA-NAGAI Kaori The University of Tokyo, 大学院薬学系研究科, 助教 (00313122)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,750,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: 抗腫瘍ワクチン / 大腸癌 / MUC1 / CD4陽性T細胞 / 臓器特異的エフェクター機構 / FasL / TNF-α / DNAワクチン / 肝転移

Research Abstract

抗原特異的抗腫瘍ワクチンにより誘導されるエフェクター機構が、臓器特異的であることを明らかにした。臓器特異的エフェクター機構は、抗原特異的なCD4陽性T細胞の臓器による違いというよりは、CD4陽性T細胞の下流の最終的なエフェクター細胞や分子が臓器の微小環境によって規定されるためと予想される。これまでに、抗腫瘍活性の臓器特異的なエフェクター機構は全く知られていなかった。新たな抗腫瘍免疫療法の開発に役立つと考えられる。

Research Products

• [Journal Article] MGL2 dermal dendritic cells are sufficient to initiate contact hypersensitivity in vivo. (2009)
  • Author(s): Kumamoto Y, Denda-Nagai K, Aida S, Higashi N, Irimura T
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] A C-type lectin MGL1/CD301a plays an anti-inflammatory role against murine experimental colitis. (2009)
  • Author(s): Saba K, Denda-Nagai K, Irimura T
  • Journal Title: Am J Pathol 174 Pages: 144-52
  • Peer Reviewed
• [Journal Article] Differential effector mechanisms induced by vaccination with MUC1 DNA in the rejection of colon carcinoma growth at orthotopic sites and metastases. (2008)
  • Author(s): Sugiura D, Aida S, Denda-Nagai K, Takeda K, Kamata-Sakurai M, Yagita H, Irimura T
  • Journal Title: Cancer Sci. 99 Pages: 2477-84
  • Peer Reviewed
• [Journal Article] The amino acids involved in the distinct carbohydrate specificities between macrophage galactose-type C-type lectins 1 and 2 (CD301a and b) of mice. (2008)
  • Author(s): Oo-Puthinan S, Maenuma K, Sakakura M, Denda-Nagai K, Tsuiji M, Shimada I, Nakamura-Tsuruta S, Hirabayashi J, Bovin NV, Irimura T
  • Journal Title: Biochim Biophys Acta. 1780 Pages: 89-100
  • Peer Reviewed
• [Journal Article] Identification and expression of human epiglycanin/MUC21: a novel transmembrane mucin. (2008)
  • Author(s): Itoh Y, Kamata-Sakurai M, Denda-Nagai K, Nagai S, Tsuiji M, Ishii-Schrade K, Okada K, Goto A, Fukayama M, Irimura T.
  • Journal Title: Glycobiology. 18 Pages: 74-83
  • Peer Reviewed
• [Journal Article] Differential effector mechanisms induced by vaccination with MUC1 DNA in the rejection of colon carcinoma growth at orthotopic sites and me tastases (2008)
  • Author(s): Sugiura D, Aida S, Denda-Nagai K, Takeda K, Kamata-Sakurai M, Yagita H, Irimura T.
  • Journal Title: Cancer Sceience 99 Pages: 2477-84
  • Peer Reviewed
• [Journal Article] Differential effector mechanisms induced by vaccination with MUC1 DNA in the rejection of colon carcinoma growth at orthotopic sites and metastases. (2008)
  • Author(s): Sugiura D, Aida S, Denda-Nagai K, Takeda K, Kamata-Sakurai M, Yagita H, Irimura T.
  • Journal Title: Cancer Science 99 Pages: 2477-84
  • Peer Reviewed
• [Journal Article] Tumor-Associated Tn-MUC1 Glycoform Is Internalized through the Macrophage Galactose-Type C-Type Lectin and Delivered to the HLA Class I and II Compartments in Dendritic Cells. (2007)
  • Author(s): Napoletano C, Rughetti A, Agervig Tarp MP, Coleman J, Bennett EP, Picco G, Sale P, Denda-Nagai K, Irimura T, Mandel U, Clausen H, Frati L, Taylor-Papadimitriou J, Burchell J, Nuti M
  • Journal Title: Cancer Res. 67 Pages: 8358-67
  • Peer Reviewed
• [Journal Article] Properties of blocking and non-blocking monoclonal antibodies specific for human macrophage galactose-type C-type lectin (MGL/ClecSF10A/CD301). (2007)
  • Author(s): Sano Y, Usami K, Izawa R, Denda-Nagai K, Higashi N, Kimura T, Suzuki N, Irimura T
  • Journal Title: J Biochem (Tokyo). 141 Pages: 127-36
  • Peer Reviewed
• [Presentation] Epiglycanin/MUC21 as a potential marker for thyroid carcinomas. (2009)
  • Author(s): Okada K, Kamata-Sakurai M, Itoh Y, Denda-Nagai K, Ogawa T, Irimura T.
  • Organizer: 2009 AACR Annual Meeting
  • Place of Presentation: Colorado Convention Center, Denver, CO, USA
  • Year and Date: 2009-04-21
• [Presentation] Unique effector mechanisms induced by vaccination with MUC1 DNA in the rejection of colon carcinoma growth at orthotopic sites and metastases. (2009)
  • Author(s): Sugiura D, Aida S, Denda-Nagai K, Kamata-Sakurai M, Takeda K, Yagita H, Irimura T.
  • Organizer: 2009 AACR Annual Meeting
  • Place of Presentation: Colorado Convention Center, Denver, CO, USA
  • Year and Date: 2009-04-19
• [Presentation] マウスエピグリカニン/Muc21のグリコフォームに特異的なモノクローナル抗体の作製と利用 (2008)
  • Author(s): 星野真由美, 伊藤智子, 伝田香里, 石井-シュラーデカトリン, 李ユリ, 岡田恭子, 乕屋聖子, 櫻井実香, 森美穂, 入村達郎
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会(BMB2008)
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-09
• [Presentation] 樹状細胞の糖鎖付加抗原の取り込みと提示に関わるマクロファージガラクトース型C型レクチン2。The macrophage galactose-type C-type lectin 2 (MGL2/CD301b) as a glycosylated antigen uptake site for presentation on dendritic cells. (2008)
  • Author(s): 伝田香里, 佐波謙吾, 森山彩野, 杉浦大祐, 入村達郎
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2008-12-03
• [Presentation] 抗原提示細胞に発現するマクロファージガラクトース型C型レクチン1による皮膚アロ移植拒絶の制御 Macrophage galactose-type calcium-type lectin 1 expressed on antigen presenting cells modulate skin allograft rejection (2008)
  • Author(s): 森山彩野, 伝田香里, 入村達郎
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2008-12-03
• [Presentation] Epiglycanin/MUC21 の甲状腺がんにおける発現解析 (2008)
  • Author(s): 岡田恭子, 伊藤佑一, 櫻井実香, 伝田香里, 小川利久, 入村達郎
  • Organizer: 第28回日本分子腫瘍マーカー研究会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-10-27
• [Presentation] Human counterpart of Epiglycanin/Muc21 as a potential marker for thyroid carcinoma (2008)
  • Author(s): Okada K, Itoh Y, Kamata-Sakurai M, Denda-Nagai K, Ogawa T, Irimura T.
  • Organizer: 36th Congress of the International Society of Oncology & BioMarkers
  • Place of Presentation: InterContinental TOKYO BAY, Japan
  • Year and Date: 2008-10-08
• [Presentation] A novel transmembrane musin Muc21/Epiglycanin : Distribution and Glycosylation (2008)
  • Author(s): Hoshino M, Denda-Nagai K, Ishii-Schrade K, Yi Y, Okada K, Toraya S, Kamata-Sakurai M, Mori M, Irimura T.
  • Organizer: 36th Congress of the International Society of Oncology & BioMarkers
  • Place of Presentation: InterContinental TOKYO BAY, JAPAN
  • Year and Date: 2008-10-08
• [Presentation] A C-type lectin,MGL/CD301,is essential for commensal bacteria to play an anti-inflammatory role in the DSS-induced colitis madel (2008)
  • Author(s): Saba K, Denda-Nagai K, Irimura T.
  • Organizer: The 10th International Symposium on Dendritic Cells
  • Place of Presentation: International Conference Center Kobe, Kobe, Japan
  • Year and Date: 2008-10-03
• [Presentation] Effector mechanisms induced by vaccination with MUC1 DNA in the rejection of colon carcinoma growth at orthotopic sites and metastases (2008)
  • Author(s): Sugiura D, Aida S, Denda-Nagai K, Kamata-Sakurai M, Takeda K, Yagita H, Irimura T.
  • Organizer: The 10th International Symposium on Dendritic Cells
  • Place of Presentation: International Conference Center Kobe, Kobe, Japan
  • Year and Date: 2008-10-03
• [Presentation] Effector mechanisms induced by vaccination with MUC1 DNA in the rejection of colon carcinoma growth at orthotopic sites and metastases (2008)
  • Author(s): Sugiura D, Aida S, Denda-Nagai K, Takeda K, Kamata-Sakurai M, Yagita H, Irimura T.
  • Organizer: DC2008
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2008-10-03
• [Presentation] Effector mechanisms induced by vaccination with MUC1 DNA in the rejection of colon carcinoma growth at orthotopic sites and metastases. (2008)
  • Author(s): Sugiura D, Aida S, Denda-Nagai K, Takeda K, Kamata-Sakurai M, Yagita H, Irimura T.
  • Organizer: DC2008
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2008-10-03
• [Presentation] Efficient presentation of glycosylated antigens taken up through the macrophage galactose-type C-type lectin 2 on bone marrow-derived dendritic cells (2008)
  • Author(s): Denda-Nagai K, Saba K, Sugiura D, Irimura T.
  • Organizer: The 10th International Symposium on Dendritic Cells
  • Place of Presentation: International Conference Center Kobe, Kobe, Japan
  • Year and Date: 2008-10-02
• [Presentation] Macrophage galactose-type calcium-type lectin 1 expressed on antigen presenting cells modulate skin allograft rejection (2008)
  • Author(s): Moriyama S, Denda-Nagai K, Irimura T.
  • Organizer: The 10th International Symposium on Dendritic Cells
  • Place of Presentation: International Conference Center Kobe, Kobe, Japan
  • Year and Date: 2008-10-02
• [Presentation] 腫瘍ムチンとしてのエピグリカニン/MUC21 の再発見 (2008)
  • Author(s): 入村達郎, 伝田香里, 櫻井実香, 李ユリ, 星野真由美, 岡田恭子, 乕屋聖子, 森美穂, イシイ.シュラーデ カトリン, 伊藤佑一, 伊藤智子, 後藤明輝, 深山正久, 小川利久
  • Organizer: 第28回日本糖質学会年会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2008-08-18
• [Presentation] 大腸マクロファージによる細菌の認識 : C 型レクチンMGL/CD301 の役割について (2008)
  • Author(s): 佐波謙吾, 伝田香里, 入村達郎
  • Organizer: Bactereial Adherence & Biofilm 第22回学術集会
  • Place of Presentation: 兵庫県立夢舞台国際会議場
  • Year and Date: 2008-07-05
• [Presentation] Characterization and functional expression of a novel transmembrane mucin Muc21/epiglycanin (2008)
  • Author(s): Yi Y, Ishii-Schrade K, Denda-Nagai K, Kamata-Sakurai M, Toraya S,Itoh T, Mori M, Irimura T.
  • Organizer: Annual Conference of the Society for Glycobiology, 第8回HGPI 国際会議
  • Place of Presentation: Renaissance Worthington Hotel, Fort Worth, Texas, USA
• [Presentation] Protecting roles of a macrophage galactose-type C-type lectin 1(MGl1/CD301a)against experimental colitis (2008)
  • Author(s): Saba K, Denda-Nagai K, Irimura T.
  • Organizer: Annual Conference of the Society for Glycobiology, 第8回HGPI国際会議
  • Place of Presentation: Renaissance Worthington Hotel, Fort Worth, Texas, USA
• [Presentation] MGL1/CD301 modulates functions of macrophages and dendritic cells through the carbohydrate recognition. (2007)
  • Author(s): Irimura T, Denda-Nagai K, Saba K.
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会/ BMB2007
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-13
• [Presentation] MGL1/CD301a has an anti-inflammatory role towards murine experimental colitis via regulation of cytokine expression. (2007)
  • Author(s): Saba K, Denda-Nagai K, Irimura T.
  • Organizer: IMMUNORIO 2007 : 13th International Congress of Immunology
  • Place of Presentation: Sao Paulo, Brazil
  • Year and Date: 2007-08-24
• [Presentation] A Novel Transmembrane Mucin MUC21. (2007)
  • Author(s): Irimura T, Denda-Nagai K, Ishii-Schrade K,Yi Y, Okada K, Kamata-Sakurai M, Itoh Y, Okada-Itoh T, Iguchi A, Tsuiji M, Nagai S, Narimatsu H, Goto A, Fukayama M, Ogawa T, Matsunaga T.
  • Organizer: The 27th Sapporo Cancer Seminar International Symposium
  • Place of Presentation: The Hokkaido University Conference Hall, Sapporo, Japan
  • Year and Date: 2007-07-12
• [Presentation] MGL/CD301, a galactose-type calcium-type lectin expressed on macrophages and dendritic cells, differentially involved in pathogenesis dependent on the type of MGL1 expressing cells with unique distributions. (2007)
  • Author(s): Irimura T, Denda-Nagai K, Higashi N, Sato K, Yuita H, Saba K. Oo-puthinan S, Maenuma K.
  • Organizer: Mucins in Health and Disease (9th International Workshop on Carninoma-associated Mucins)
  • Place of Presentation: Robinson College, Cambridge, UK
  • Year and Date: 2007-07-08
• [Presentation] identification and characterization of mouse/human Muc21. (2007)
  • Author(s): Ishii-Schrade, KB, Kamata-Sakurai M, Itoh Y, Yi Y, Iguchi A, Okada K, Denda-Nagai K, Nagai S, Tsuiji M, Irimura T.
  • Organizer: Mucins in Health and Disease(9th International Workshop on Carninoma-associated Mucins)
  • Place of Presentation: Robinson College, Cambridge, UK
  • Year and Date: 2007-07-08
• [Presentation] MGL/CD301, a galactose-type calcium-type lectin expressed on macrophages and dendritic cells, differentially involved in pathogenesis dependent on the organ distributions of these cells. (2007)
  • Author(s): Irimura T, Denda-Nagai K, Higashi N, Sato K, Yuita H, Saba K. Oo-puthinan S, Maenuma K.
  • Organizer: 16th International Symposium on Molecular Cell Biology of Macrophages 2007
  • Place of Presentation: Shizuoka Convention & Arts Center Shizuoka, Japan
  • Year and Date: 2007-06-15
• [Presentation] MGL1/CD301a has an anti-inflammatory role towards murine experimental colitis via regulation of cytokine expression. (2007)
  • Author(s): Saba K, Denda-Nagai K, Irimura T.
  • Organizer: 16th International Symposium on Molecular Cell Biology of Macrophages 2007
  • Place of Presentation: Shizuoka Convention & Arts Center Shizuoka, Japan
  • Year and Date: 2007-06-15
• [Book] 糖鎖を利用した癌の免疫療法、第3の生命鎖:糖鎖の謎が今、解る(古川鋼一 編) (2009)
  • Author(s): 入村達郎, 伝田香里, 杉浦大祐
  • Publisher: 株式会社クバプロ
• [Book] 糖鎖を利用した癌の免疫療法」、『第3の生命鎖 : 糖鎖の謎が今、解る(古川鋼一編) (2009)
  • Author(s): 入村達郎、伝田香里、杉浦大祐
  • Publisher: 株式会社クバプロ
• [Book] Detection of Mouse MGL1/CD301a by a Specific Monoclonal Antibody. (2008)
  • Author(s): Denda-NAGAI K, Suzuki K, Goda M, Kudo A, Kawakami H, Irimura, T.
  • Publisher: Expereimental Glycoscience.