| Budget Amount *help |
¥3,710,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Research Abstract |
Cytochrome P450 (CYP) is one of the most important drug-metabolizing enzymes. In this study, we investigated the effects of protein/protein interaction on the enzyme activity of CYPs. Mammalian two-hybrid system and immunoprecipitation assay revealed that CYP3A4 interacted with CYP3A4 or CYP3A5 in the cells transefected with expression plasmids for myc- or flag-tagged proteins. CYP3A4 also interacted with CYP2C isoforms. These results suggest the CYPs interact with each other in the cells, and possibly these interactions were associated with CYP expression or function.
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