Development of fiber modified adenovirus vector

• Project/Area Number: 19790135
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Medical pharmacy
• Research Institution: Showa Pharmaceutical University
• Principal Investigator: KOIZUMI Naoya Showa Pharmaceutical University, 薬学部, 助教 (80433845)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: ドラックデリバリー / 遺伝子治療 / ターゲティング / 薬学 / 再生医療

Research Abstract

遺伝子治療の更なる発展のためには、既存の遺伝子導入ベクターを詳細に解析し、さらに有用なベクター開発につなげることが必要である。そこで、高い遺伝子導入効率を持つアデノウイルスベクターを解析し、ウイルスタンパク質であるファイバーシャフト部分が効率的遺伝子導入に関与していることを見出した。さらに、シャフト蛋白質は遺伝子を搭載したプラスミドとプラスミド導入試薬による遺伝子発現効率を約10倍上昇することを明らかとした。

Research Products

• [Journal Article] The modification of the pIX or hexon based on fiberless Ad vectors is not effective for the development of targeted Ad vectors (2008)
  • Author(s): Kurachi S., Koizumi N., Tashiro K., Sakurai H., Sakurai F., Kawabata K., Nakagawa S., Mizuguchi H
  • Journal Title: J. Control. Release. 1 Pages: 88-95
  • Peer Reviewed
• [Journal Article] Modification of pIX or hexon based on fiberless Ad vectors is not effective for targeted Ad vectors. (2008)
  • Author(s): Kurachi, S・Koizumi, N, et. al.
  • Journal Title: J Control Release. 1 Pages: 88-95
  • Peer Reviewed
• [Journal Article] Role of MyD88 and TLR9 in the Innate Immune Response Elicited by Serotype 5 Adenovirus Vectors. (2007)
  • Author(s): Yamaguchi T., Kawabata K., Koizumi N., Sakurai F., Nakashima K., Sakurai H., Sasaki T., Okada N, Yamanishi K., Mizuguchi H
  • Journal Title: Hum. Gene Ther. 18 Pages: 753-762
  • Peer Reviewed
• [Journal Article] Role of MyD88 and TLR9 in the innate immune response elicited by serotype 5 adenoviral vectors. (2007)
  • Author(s): Yamaguchi, T・Kawabata, K・Koizumi, N, et. al.
  • Journal Title: Hum Gene Ther. 8 Pages: 753-762
  • Peer Reviewed
• [Presentation] アデノウイルスベクターを用いたCaco-2単層膜細胞への効率的遺伝子導入方法の開発遺伝子デリバリー研究会 (2008)
  • Author(s): 山岸喜彰、小泉直也、水口裕之、藤井まき子、渡辺善照
  • Organizer: 第8回シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2008-06-09
• [Presentation] アデノウイルスベクターを用いたCaco-2単層膜細胞への効率的遺伝子導入方法の開発 (2008)
  • Author(s): 山岸喜彰, 小泉直也, 水口裕之, 藤井まき子, 渡辺善照
  • Organizer: 遺伝子デリバリー研究会第8回シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2008-05-19
• [Presentation] DEVELOPMENT OF EFFICIENT GENE TRANSFER SYSTEMS INTO CACO-2 CELL MONOLAYER BY ADENOVIRUS VECTOR (2008)
  • Author(s): Naoya Koizumi, Yoshiaki Yamagishi, Hiroyuki Mizuguchi, Makiko Fujii, Yoshiteru Watanabe
  • Organizer: 4th Pan-Pacific International Partnership Conference on Pharmaceutical and Life Sciences
  • Place of Presentation: 名古屋
• [Presentation] アデノウイルスカプシド蛋白質を利用した効率的遺伝子導入法の開発 (2008)
  • Author(s): 小泉直也、萩原恵理、岩島順子、山岸喜彰、水口裕之、藤井まき子、渡辺善照
  • Organizer: 第24回日本DDS学会
  • Place of Presentation: 東京
• [Presentation] DEVELOOPMENT OF EFFICIENT GENE TRANSFER SYSTEMS INTO CACO-2 CELL MONOLAYER BY ADENOVIRUS VECTOR (2008)
  • Author(s): Naoya, Koizumi・Tomomi, Sasaki・Hiroyuki, Mizuguchi・Makiko, Fujii・Yoshiteru, Watanabe
  • Organizer: 4th Pan-Pacific International Partnership Conference on Pharmaceutical and Life Sciences
  • Place of Presentation: 愛知県名古屋市
• [Presentation] Infectious mechanism of adenovirus vector in Caco-2 cell for permeability study using a transgene expressed intestinal epithelium model (2007)
  • Author(s): Naoya Koizumi, Tomomi Sasaki, Hiroyuki Mizuguchi, Makiko Fujii, Yoshiteru Watanabe
  • Organizer: 8th International Society for the Study of Xenobiotics Meeting
  • Place of Presentation: 仙台
• [Presentation] Infectious mechanism of adenovirus vector in Caco-2 cell for permeability study using a transgene expressed intestinal epithelium model (2007)
  • Author(s): Naoya, Koizumi・Tomomi, Sasaki・Hiroyuki, Mizuguchi・Makiko, Fujii・Yoshiteru, Watanabe
  • Organizer: 8th International Society for the Study of Xenobi otics Meeting
  • Place of Presentation: 宮城県仙台市