Development of metastatic breast cancer therapies with anticancer drug-loaded inorganic nanoparticles targeted to lymph nodes

• Project/Area Number: 19790143
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Medical pharmacy
• Research Institution: Fujita Health University
• Principal Investigator: MURAKAMI Tatsuya Fujita Health University, 物質-細胞統合システム拠点, 特定拠点助教 (90410737)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥540,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: ドラッグデリバリー / ナノ粒子 / カーボンナノチューブ / 癌化学療法 / 乳癌 / リンパ節転移 / 腋窩リンパ節 / ドラッグキャリア

Research Abstract

Novel drug-drug carrier complexes with a new type of nanocarbon material, carbon nanohorn (CNH), have been developed. CNH was easily loaded with an anticancer drug, doxorubicin (DXR). The complex slowly released DXR, which would contribute to enhancement of the antitumor activity. When CNH was loaded with an photosensitizer, zinc phthalocyanine (ZnPc), the complex enabled double photodynamic and photothermal cancer therapies. More importantly, CNH administered into tumor-bearing mice was able to accumulate in the adjacent axial lymph nodes, which are well known to be an organ in which breast cancer metastasis often occurs.

Research Products

• [Journal Article] Photoinduced electron transfer in zinc phthalocyanine loaded on single-walled carbon nanohorns in aqueous solution. (2009)
  • Author(s): A.S.D. Sandanayaka, O. Ito, M. Zhang, K. Ajima, S. Iijima, M. Yudasaka, T. Murakami, K. Tsuchida
  • Journal Title: Adv. Mater 21 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Biodistribution and ultrastructural localization of single-walled carbon nanohorns determined in vivo with embedded Gd2O3 labels. (2009)
  • Author(s): J. Miyawaki, S. Matsumura, R. Yuge, T. Murakami, S. Sato, A. Tomida, T. Tsuruo, T. Ichihashi, T. Fujinami, H. Irie, K. Tsuchida, S. Iijima, K. Shiba, M. Yudasaka
  • Journal Title: ACS Nano 3 Pages: 1399-1406
  • Peer Reviewed
• [Journal Article] Biodistribution and ultrastructural localization of single-walled carbon nanohorns determined in vivo with embedded Gd203 labels (2009)
  • Author(s): Miyawaki, J., Matsumura, S., Yuge, R., Murakami, T., Sato, S., Tomida, A., Tsuruo, T., Ichihashi, T., Fujinami, T., Irie, R., Tsuchida, K., Iijima, S., Shiba, K., Yudasaka, M.
  • Journal Title: ACS Nano 3 Pages: 1399-1406
  • Peer Reviewed
• [Journal Article] Photoinduced electron transfer in zinc phthalocyanine loaded on single-walled carbon nanohorns in aqueous solution (2009)
  • Author(s): Sandanayaka, A.S.D., Ito, O., Zhang, M., Ajima, K., Iijima, S., Yudasaka, M., Murakami, T., Tsuchida, K.
  • Journal Title: Adv.Mater. 21 Pages: 1-6
  • Peer Reviewed
• [Journal Article] 生体ナノ粒子を用いたドラッグキャリア開発 (2009)
  • Author(s): 村上達也
  • Journal Title: Drug Delivery System 24 Pages: 636-637
• [Journal Article] Fabrication of ZnPc/protein nanohorns for double photodynamic and hyperthermic cancer phototherapy. (2008)
  • Author(s): M. Zhang, T. Murakami, K. Ajima, K. Tsuchida, A.S. Sandanayaka, O. Ito, S. Iijima, M. Yudasaka
  • Journal Title: Proc. Natl. Acad. Sci. USA 105 Pages: 14773-14778
  • Peer Reviewed
• [Journal Article] Enhancement of in vivo anticancer effects of cisplatin by incorporation inside single-wall carbon nanohorns. (2008)
  • Author(s): K. Ajima, T. Murakami, Y. Mizoguchi, K. Tsuchida, T. Ichihashi, S. Iijima, M. Yudasaka
  • Journal Title: ACS Nano 2 Pages: 2057-2064
  • Peer Reviewed
• [Journal Article] (2008)
  • Author(s): T. Murakami, H. Sawada, G. Tamura, M. Yudasaka, S. Iijima, K. Tsuchida
  • Journal Title: Nanomed 3 Pages: 453-463
  • Peer Reviewed
• [Journal Article] Fabrication of ZnPc/protein nanohorns for double photodynamic and hyperthermic cancer phototherapy (2008)
  • Author(s): Zhang, M
  • Journal Title: Proc. Natl. Acad. Sci. U. S. A 105 Pages: 14773-14778
  • Peer Reviewed
• [Journal Article] Enhancement of in vivo anticancer effects of cicplatin by incorporation inside single-wall carbon nanohorns (2008)
  • Author(s): Ajima, K
  • Journal Title: ACS Nano 2 Pages: 2057-2064
  • Peer Reviewed
• [Journal Article] Water-dispersed single-wall carbon nanohorns as drug carriers for local cancer chemotherapy (2008)
  • Author(s): Murakami, T
  • Journal Title: Nanomedicine 3 Pages: 453-463
  • Peer Reviewed
• [Journal Article] Signal transduction pathway through activin receptors as a therapeutic target of musculoskeletal diseases and cancer (2008)
  • Author(s): Tsuchida, K.
  • Journal Title: Endocrine J. 55 Pages: 11-21
  • Peer Reviewed
• [Journal Article] Characterization of follistatin-related gene as a negative regulatory factor for activin family members during heart development (2007)
  • Author(s): Takehara-Kusamatsu, Y.
  • Journal Title: J. Med. Invest. 54 Pages: 276-288
  • Peer Reviewed
• [Journal Article] Transgenic expression of a myostatin inhibitor derived from follistatin increases skeletal muscle mass and ameliorates dystrophic pathology in mdx mice. (2007)
  • Author(s): Nakatani, M.
  • Journal Title: FASEB J. 22 Pages: 477-487
  • Peer Reviewed
• [Presentation] リポタンパク質変異体による抗癌剤の細胞内デリバリー (2010)
  • Author(s): 村上達也
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: ホテルグランビア岡山
  • Year and Date: 2010-03-29
• [Presentation] Intracellular drug delivery by cell-penetrating high density lipoprotein (2009)
  • Author(s): Tatsuya Murakami
  • Organizer: 10th US-Japan symposium on drug delivery systems
  • Place of Presentation: ウェスティンマウイ(米国)
  • Year and Date: 2009-12-18
• [Presentation] ナノ粒子の表面修飾による生体適合化とドラッグデリバリーシステムへの応用 (2009)
  • Author(s): 村上達也
  • Organizer: 日本接着学会東北支部講演会2009
  • Place of Presentation: 東北大学
  • Year and Date: 2009-11-13
• [Presentation] ナノ粒子の表面修飾による生体適合化とドラッグデリバリーシステムへの応用 (2009)
  • Author(s): 村上達也
  • Organizer: 日本接着学会東北支部講演会2009
  • Place of Presentation: 東北大学片平キャンパス
  • Year and Date: 2009-11-13
• [Presentation] Intracellular drug delivery by genetically engineered high density lipoprotein (2009)
  • Author(s): Tatsuya Murakami
  • Organizer: The fifth iCeMS international symposium on integrated cell-material sciences
  • Place of Presentation: 京都大学iCeMS本館
  • Year and Date: 2009-07-27
• [Presentation] 遺伝子工学を利用した細胞侵入型リポタンパク質ナノ粒子の作製 (2009)
  • Author(s): 村上達也
  • Organizer: 第25回日本DDS学会
  • Place of Presentation: 東京ドームホテル
  • Year and Date: 2009-07-03
• [Presentation] Development of smart drug carriers based on a naturally occurring nanoparticle (2009)
  • Author(s): Tatsuya Murakami
  • Organizer: The fourth iCeMS international symposium on integrated cell-material sciences
  • Place of Presentation: ホテルフジタ京都
  • Year and Date: 2009-05-27
• [Presentation] DDS応用を目指したリポ蛋白質の高機能化 (2008)
  • Author(s): 村上達也
  • Organizer: 第24回日本DDS学会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-30
• [Presentation] 蛋白質工学的手法を駆使した機能性リボ蛋白質の作製 (2008)
  • Author(s): 村上達也
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-26
• [Presentation] 抗癌活性を有するナノ粒子カーボンナノホーンのin vitroおよびin vivo機能評価 (2007)
  • Author(s): 村上達也
  • Organizer: BMB2007
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2007-12-14
• [Presentation] 抗癌活性を有するナノ粒子カーボンナノホーンのin vitroおよびin vivo機能評価 (2007)
  • Author(s): 村上達也
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] 局所投与用ドラッグキャリアとしてのカーボンナノホーン (2007)
  • Author(s): 村上達也
  • Organizer: 第29回日本バイオマテリアル学会
  • Place of Presentation: 千里ライフサイエンスセンター(大阪)
  • Year and Date: 2007-11-26
• [Presentation] 局所投与用ドラッグキャリアとしてのカーボンナノホーン (2007)
  • Author(s): 村上達也
  • Organizer: 日本バイオマテリアル学会
  • Place of Presentation: 大阪
  • Year and Date: 2007-11-26
• [Presentation] 抗癌剤担持水溶性カーボンナノホーンの薬理評価 (2007)
  • Author(s): 村上達也
  • Organizer: 第23回日本DDS学会
  • Place of Presentation: 熊本日航ホテル(熊本)
  • Year and Date: 2007-06-14
• [Presentation] 抗癌剤担持水溶性カーボンナノホーンの薬理評価 (2007)
  • Author(s): 村上達也
  • Organizer: 第23回日本DDS学会
  • Place of Presentation: 熊本
  • Year and Date: 2007-06-14
• [Remarks]
  • URL: http://www.icems.kyoto-u.ac.jp/j/ppl/grp/murakami.html