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CLIC4 channel regulates cell proliferation via changes in intranuclear Clconcentration

Research Project

Project/Area Number 19790168
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

MIYAZAKI Hiroaki  Kyoto Prefectural University of Medicine, 医学研究科, 助教 (30360027)

Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywords核 / Cl-濃度 / CLIC4 / 細胞増殖 / 細胞周期 / C1^-濃度 / C1濃度
Research Abstract

胃ガン由来細胞株であるMKN28細胞を低Cl-濃度培地で培養を行ったところ、細胞周期のG1期からS期への進行が抑制されることが明らかとなった。また低Cl-処理は、mitogen-activated protein kinase (MAPK)の活性化を誘発することで、細胞周期のG1期からS期の進行を抑制するp21タンパクの発現を亢進することが明らかになった。MAPKを介した転写制御は核内で起こることや、p21などの細胞周期調節タンパク質は細胞の核内で機能していることから、細胞質内のCl-濃度変化が核膜に存在するCl-チャネル(CLIC4)を介し核内のCl-濃度に対しても大きく影響を与え、その結果核内の細胞周期調節タンパクの活性に影響を与えているものと考えられた。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (25 results)

All 2009 2008 2007

All Journal Article (15 results) (of which Peer Reviewed: 15 results) Presentation (10 results)

  • [Journal Article] Regulation of paracellular Na+ and Clconductances by hydrostatic pressure.2009

    • Author(s)
      Tokuda, S., Niisato, N., Nagai, T., Taruno, T., Nakajima, K., Miyazaki, H., Yamada, T., Hosogi, S., Oha, M., Nishio, K., Iwasaki, Y., and Marunaka, Y.
    • Journal Title

      Cell Biology International (in press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quercetin stimulates Na+/K+/2Clcotransport via PTK-dependent mechanisms in human airway epithelium.2009

    • Author(s)
      Asano, J., Niisato, N., Nakajima, K., Miyazaki, H., Yasuda, M., Iwasaki, Y., Hama, T., Dejima, K., Hisa, Y., and Marunaka, Y.
    • Journal Title

      American Journal of Respiratory Cell and Molecular Biology. (in press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Blockers of K+/Cl- transporter/channels diminish proliferation of osteoblastic cells.2009

    • Author(s)
      Maki, M., Miyazaki, H., Niisato, N., Morihara, T., Marunaka, Y., and Kubo, T.
    • Journal Title

      Biomedical Research. 30(2)

      Pages: 137-140

    • NAID

      130004470739

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Blockers of K^+/C1^-transporter/channels diminish proliferation of osteoblastic cells2009

    • Author(s)
      Maki, M
    • Journal Title

      Biomedical Research 30

      Pages: 137-140

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chloride ions control the G1/S cell-cycle checkpoint by regulating the expression of p21 through a p53-independent pathway in human gastric cancer cells.2008

    • Author(s)
      Miyazaki, H., Shiozaki, A., Niisato, N., Ohsawa, R., Itoi, H., Ueda, Y., Yamagishi, H., Iwasaki, Y., Nakano, T., Nakahari, T., and Marunaka, Y.
    • Journal Title

      Biochemical and Biophysical Research Communications. 366(2)

      Pages: 506-512

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chloride ions control the G_1/S cell-cycle checkpoint by regulating the expression of p21 through a p53-independent pathway in human gastric cancer cells.2008

    • Author(s)
      Miyazaki, H.
    • Journal Title

      Biochemical and Biophysical Research Communications 366

      Pages: 506-512

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 22,Epithelial Na+ channel and ion transport in human nasal polyp and paranasal sinus mucosa.2007

    • Author(s)
      Yasuda, M., Niisato, N., Miyazaki, H., Iwasaki, Y., Hama, T., Dejima, K., Hisa, Y., and Marunaka, Y.
    • Journal Title

      Biochemical and Biophysical Research Communications. 362(3)

      Pages: 753-758

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chloride-dependent acceleration of cell cycle via modulation of Rb and cdc2 in osteoblastic cells.2007

    • Author(s)
      Maki, M., Miyazaki, H., Nakajima, K., Yamane, J., Niisato, N., Morihara, T., Kubo, T., and Marunaka, Y.
    • Journal Title

      Biochemical and Biophysical Research Communications. 361(4)

      Pages: 1038-1043

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Essential role of NKCC1 in NGF-induced neurite outgrowth.2007

    • Author(s)
      Nakajima, K., Miyazaki, H., Niisato, N., and Marunaka, Y.
    • Journal Title

      Biochemical and Biophysical Research Communications. 359(3)

      Pages: 604-610

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] An inhaled inducible nitric oxide synthase inhibitor reduces damage of Candida-induced acute lung injury.2007

    • Author(s)
      Ohsugi, S., Iwasaki, Y., Takemura, Y., Nagata, K., Harada, H., Yokomura, I., Hosogi, S., Yuba, T., Niisato, N., Miyazaki, H., Matsubara, H., Fushiki, S., and Marunaka, Y.
    • Journal Title

      Biomedical Research. 28(2)

      Pages: 91-99

    • NAID

      130004903735

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Abnormal expression of ENaC and SGK1 mRNA induced by dietary sodium in Dahl salt-sensitively hypertensive rats.2007

    • Author(s)
      Aoi, W., Niisato, N., Sawabe, Y., Miyazaki, H., Tokuda, S., Nishio, K., Yoshikawa, T., and Marunaka, Y.
    • Journal Title

      Cell Biology International. 31(10)

      Pages: 1288-1291

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Action of neltenexine on anion secretion in human airway epithelia.2007

    • Author(s)
      Niisato, N., Hasegawa, I., Tokuda, S., Taruno, A., Nakajima, K., Miyazaki, H., Iwasaki, Y., and Marunaka, Y.
    • Journal Title

      Biochemical and Biophysical Research Communications. 356(4)

      Pages: 1050-1055

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Physiological significance of hypotonicityinduced regulatory volume decrease : reduction in intracellular Cl- concentration acting as an intracellular signaling.2007

    • Author(s)
      Miyazaki, H., Shiozaki, A., Niisato, N., and Marunaka, Y.
    • Journal Title

      American Journal of Physiology-Renal Physiology. 292(5)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Physiological significance of hypotonicity-induced regulatory volume decrease:reduction in intracellular CI concentration acting as an intracellular signaling.2007

    • Author(s)
      Miyazaki, H.
    • Journal Title

      American Journal of Physiology, Renal Physiology 292

      Pages: 1411-1417

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chloride-dependent acceleration of cell cycle via modulation of Rb and cdc2 in osteoblastic cells2007

    • Author(s)
      Maki, M.
    • Journal Title

      Biochemical and Biophysical Research Communications 361

      Pages: 1038-1043

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] ヒト胃癌細胞株において細胞内crは細胞周期制御シグナルとして作用する2008

    • Author(s)
      宮崎裕明
    • Organizer
      膜シンポジウム2008
    • Place of Presentation
      大阪大学
    • Year and Date
      2008-11-15
    • Related Report
      2008 Annual Research Report
  • [Presentation] Intracellular chloride is a key regulator of the G1/S transition in MKN28 human gastric cancer cells2008

    • Author(s)
      Miyazaki, H
    • Organizer
      Beatson International Cancer Conference
    • Place of Presentation
      Glasgow, Scotland
    • Year and Date
      2008-06-16
    • Related Report
      2008 Annual Research Report
  • [Presentation] ヒト胃癌細胞株において細胞内CI濃度はG_1/S細胞周期チェックポイントを制御する2008

    • Author(s)
      宮崎 裕明
    • Organizer
      第85回日本生理学会大会
    • Place of Presentation
      京王プラザホテル(東京)
    • Year and Date
      2008-03-27
    • Related Report
      2007 Annual Research Report
  • [Presentation] 調節性容積減少(RVD)によって引き起こされる細胞内Cl-濃度低下は細胞内シグナルとして機能する2008

    • Author(s)
      宮崎裕明,新里直美,丸中良典
    • Organizer
      第46回生物物理学会年回
    • Place of Presentation
      福岡
    • Related Report
      2008 Final Research Report
  • [Presentation] ヒト胃癌細胞株において細胞内Cl-は細胞周期制御シグナルとして作用する2008

    • Author(s)
      宮崎裕明,大澤るみ,新里直美,丸中良典
    • Organizer
      膜シンポジウム2008
    • Place of Presentation
      豊中
    • Related Report
      2008 Final Research Report
  • [Presentation] ヒト胃癌細胞株における細胞内Cl-濃度低下によって引き起こされるG1/S細胞周期遅延はMAPKを介して調節される2008

    • Author(s)
      宮崎裕明,大澤るみ,新里直美,丸中良典
    • Organizer
      第3回トランスポーター研究会
    • Place of Presentation
      京都
    • Related Report
      2008 Final Research Report
  • [Presentation] Intracellular chloride is a key regulator of the G1/S transition in MKN28 human gastric cancer cell.2008

    • Author(s)
      Miyazaki, H., Ohsawa, R., Shiozaki, A., Niisato, N., and Marunaka, Y.
    • Organizer
      Beatson International Cancer Conference
    • Place of Presentation
      Glasgow、Scotland
    • Related Report
      2008 Final Research Report
  • [Presentation] 調節性容積減少(RVD)によって引き起こされる細胞内Cl-濃度低下は細胞内シグナルとして機能する2008

    • Author(s)
      宮崎裕明,新里直美,西尾恭介,丸中良典
    • Organizer
      第51回日本腎臓学会学術総会
    • Place of Presentation
      福岡
    • Related Report
      2008 Final Research Report
  • [Presentation] ヒト胃癌細胞株において細胞内Cl-濃度はG1/S細胞周期チェックポイントを制御する2008

    • Author(s)
      宮崎裕明,大澤るみ,塩崎敦,新里直美,丸中良典
    • Organizer
      第85回日本生理学会大会
    • Place of Presentation
      東京
    • Related Report
      2008 Final Research Report
  • [Presentation] 骨芽細胞におけるクロライド依存的な細胞周期調節2007

    • Author(s)
      宮崎裕明,牧昌弘,中島謙一,新里直美,丸中良典
    • Organizer
      第100回近畿生理学談話会
    • Place of Presentation
      津
    • Related Report
      2008 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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