Functional analysis of phospholipase C-zeta-deficient mouse

• Project/Area Number: 19790170
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: Yamaguchi University (2009); Tokyo Women's Medical University (2007-2008)
• Principal Investigator: ITO Masahiko Tokyo Women's Medical University, 総合科学実験センター, 助教 (50385423)
• Co-Investigator(Renkei-kenkyūsha): MIYAZAKI Shunichi 東京女子医科大学, 学長 (80010081) ; MIYADO Kenji 国立成育医療研究センター研究所, 生殖医療研究部, 室長 (60324844)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,830,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥630,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,100,000 (Direct Cost: ¥1,100,000)
• Keywords: ホスホリパーゼCゼータ / 卵活性化精子因子 / カルシウムオシレーション / 遺伝子欠損マウス / 精子形成 / 受精

Research Abstract

In mammalian fertilization, Ca^<2+> oscillations are indispensable for egg- activation. Sperm specific isotype of phospholopase C (PLCZ1) is a strong candidate for egg-activating sperm factor. In this study, regulation of Ca^<2+> oscillations by PLCZ1 was examined about nuclear localization ability and Ca^<2+> oscillation inducing activity. Moreover, PLCZ1 deficient mice were analyzed and it was shown that PLCZ1 has a pivotal role for spermatogenesis as well as an egg activation.

Research Products

• [Journal Article] Relationship between Nuclear sequestration of PLCzeta and termination of PLCzeta-induced Ca2+ oscillations in mouse eggs. (2008)
  • Author(s): Masahiko Ito, Tomohide Shikano, Keiji Kuroda, Shunichi Miyazaki.
  • Journal Title: Cell Calcium 44 Pages: 400-410
  • Peer Reviewed
• [Journal Article] Difference in Ca2+ oscillation - inducing activity and nuclear translocation ability of PLCZ1, an egg - activating sperm factor candidate, among mouse, rat, human and medaka fish. (2008)
  • Author(s): Masahiko Ito, Tomohide Shikano, Shoji Oda, Takashi Horiguchi, Satomi Tanimoto, Takeo Awaji, Hiroshi Mitani, Shunichi Miyazaki.
  • Journal Title: Biol Reprod 78 Pages: 1081-1090
  • Peer Reviewed
• [Journal Article] Difference in Ca^<2+> oscillation-inducing activity and nuclear translocation ability of PLCZ1, an egg-activating sperm factor candidate, among mouse, rat, human and medaka fish (2008)
  • Author(s): Masahiko Ito, et.al.
  • Journal Title: Biol. Reprod 78 Pages: 1081-1090
  • Peer Reviewed
• [Journal Article] Relationship between Nuclear Sequestration of PLCzeta and Termination of PLCzeta-induced Ca^<2+> Oscillations in Mouse Eggs (2008)
  • Author(s): Masahiko Ito, et.al.
  • Journal Title: Cell Calcium 44 Pages: 400-410
  • Peer Reviewed
• [Presentation] Analysis of negative feedback mechanism of phospholipase C-zeta, a egg-activating sperm factor candidate, overexpression (2009)
  • Author(s): 伊藤昌彦、鹿野朝秀、村田智昭、宮崎俊一
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] Analysis of negative feedback mechanism of phospholipase C-zeta, a egg-activating spermfactor candidate, overexpression (2009)
  • Author(s): 伊藤昌彦
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神奈川県・パシフィコ横浜
  • Year and Date: 2009-12-12
• [Presentation] 卵活性化精子因子候補ホスホリパーゼCゼータの核移行能の役割 (2007)
  • Author(s): 伊藤 昌彦
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-12
• [Presentation] 卵活性化精子因子候補ホスホリパーゼCゼータの核移行能の役割 (2007)
  • Author(s): 伊藤昌彦、鹿野朝秀、堀口崇、谷本さとみ、三谷啓志、尾田正二、淡路健雄、宮崎俊一
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-09
• [Presentation] 卵活性化精子因子候補ホスホリパーゼCゼータの核移行能の役割 (2007)
  • Author(s): 伊藤昌彦
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会、ワークショップ(受精直後の分子生物学)
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-09