Brain neurosteroids synthesized in the neonatal brain modulate the construction of neural circuits.

• Project/Area Number: 19790181
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: Nippon Medical School
• Principal Investigator: ISHII Hirotaka Nippon Medical School, 大学院医学研究科, ポストドクター (20445810)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,550,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: 神経内分泌学 / 生殖生理学 / 生物物理学 / 脳ニューロステロイド / エストロゲン / GnRH / アロプレグナノロン / GABA_A受容体 / ステロイドホルモン / メラトニン / アロプレグネノロン

Research Abstract

性腺刺激ホルモン放出ホルモン(GnRH)を分泌する神経細胞であるGnRHニューロンは、幼弱期神経細胞様の特徴を持った神経細胞である。本研究は、幼弱期神経細胞のモデルとしてGnRHニューロンを用い、脳ニューロステロイドであるアロプレグナノロンやエストロゲンの作用機構の解析を行った。さらに、脳ニューロステロイド合成の調節因子として知られるメラトニンの作用を解明するため、その受容体を同定し、受容体の発現調節機構・作用機構の解析を行った。

Research Products

• [Journal Article] Ca2+ channels and Ca2+-activated K+channels in adult rat gonadotropin-releasing hormone neurons (2009)
  • Author(s): 加藤昌克、田中伸幸、石井寛高、尹成珠、佐久間康夫
  • Journal Title: J.Neuroendocrinol 21(4) Pages: 312-315
  • Peer Reviewed
• [Journal Article] Gene structures, biochemical characterization and distribution of rat melatonin receptors (2009)
  • Author(s): 石井寛高、田中伸幸、小林桃子、加藤昌克、佐久間康夫
  • Journal Title: J.Physiol.Sci 59(1) Pages: 37-47
  • NAID: 10025218139
  • Peer Reviewed
• [Journal Article] Gene structures, biochemical characterization and distribution of rat melatonin recentors (2009)
  • Author(s): 石井寛高
  • Journal Title: Journal of Physiological Sciences 59 Pages: 37-47
  • Peer Reviewed
• [Journal Article] Ca^<2+> channels and Ca^<2+>-activated K^+ channels in adult rat gonadotropin-releasi ne hormone neurons (2009)
  • Author(s): 加藤昌克
  • Journal Title: Journal of Neuroendocrinoloev 21 Pages: 312-315
  • Peer Reviewed
• [Journal Article] Gonadotropin-Releasing Hormone Antagonist, Cetrorelix, Induces the Expression of Melatonin Receptor la (MT1) in the GnRH Neuronal Cell Line GT1-7 (2009)
  • Author(s): 石井寛高
  • Journal Title: Neuroendocrinology (In press)
  • Peer Reviewed
• [Journal Article] Estrogen synthesis in the brain-role in synaptic plasticity and memory (2008)
  • Author(s): 北条泰嗣、村上元、向井秀夫、肥後心平、畑中悠佑、荻上(池田)真理、石井寛高、木本哲也、川戸佳
  • Journal Title: Mol Cell Endocrinol 290(1-2) Pages: 31-43
  • Peer Reviewed
• [Journal Article] Local Production of Estrogen and its Rapid Modulatory Action on Synaptic Plasticity (2008)
  • Author(s): 川戸佳、北条泰嗣、向井秀夫、村上元、荻上(池田)真理、石井寛高、木本哲也
  • Journal Title: in Neuroactive Steroids in Brain Function, Behavior and Neuropsychiatric Disorders Pages: 143-169
  • Peer Reviewed
• [Journal Article] Activation of A-type γ-amino butyric acid receptors (GABAARs) excites gonadotropin-releasing hormone (GnRH) neurons isolated from adult rats (2008)
  • Author(s): 尹成珠、石井寛高、田中伸幸、佐久間康夫、加藤昌克
  • Journal Title: J.Neuroendocrinol. 20(5) Pages: 566-575
  • Peer Reviewed
• [Journal Article] Rat GnRH neurons exhibit large conductance voltage- and Ca2+-activated K+(BK) currents and express BK channel mRNAs (2008)
  • Author(s): 平泉良枝、西村一路、石井寛高、田中伸幸、竹下俊行、佐久間康夫、加藤昌克
  • Journal Title: J.Physiol.Sci 58(1) Pages: 21-29
  • NAID: 10022597577
  • Peer Reviewed
• [Journal Article] 17β-estradiol at physiological concentrations augments Ca2+-activated K+currents via estrogen receptor β in the GnRH neuronal cell line GT1-7 (2008)
  • Author(s): 西村一路、宇井-程久美子、西郷薫、石井寛高、佐久間康夫、加藤昌克
  • Journal Title: Endocrinology 149(2) Pages: 774-782
  • Peer Reviewed
• [Journal Article] Activation of A-type γ-amino butyric acid receptors (GABA_ARs) excitesgonado tronin-releasine hormone (GnRH) neurons isolated from adult rats (2008)
  • Author(s): 尹成珠
  • Journal Title: Journal of Neuroendocrinology 20 Pages: 566-575
  • Peer Reviewed
• [Journal Article] Local Production of Estrogen and its Rapid Modulatory Action on Synaptic P1 asticity (2008)
  • Author(s): 川戸佳
  • Journal Title: Neuroactive Steroids in Brain Function, Behavior and N euronsvchiatric Disorders Pages: 143-169
  • Peer Reviewed
• [Journal Article] Estrogen synthesis in the brain-role in synaptic plasticity and memory (2008)
  • Author(s): 北條泰嗣
  • Journal Title: Molecular Cellular Endocrinology 290 Pages: 31-43
  • Peer Reviewed
• [Journal Article] 17β-estradiol at physiological concentrations augments Ca^<2+>-activated K^+ currents via estrogen receptor β in the GnRH neuronal cell line GT1-7 (2008)
  • Author(s): 西村 一路
  • Journal Title: Endocrinology 149 Pages: 774-782
  • Peer Reviewed
• [Journal Article] Rat GnRH neurons exhibit large conductance voltage-and Ca^<2+>-activated K^+ (BK) currents and express BK channel mRNAs (2008)
  • Author(s): 平泉 良枝
  • Journal Title: Journal of Physiological Sciences 58 Pages: 21-29
  • Peer Reviewed
• [Journal Article] Local production of sex hormones and their modulation of hippocampal synaptic plasticity. (2007)
  • Author(s): 石井寛高、釣木澤朋和、荻上(池田)真理、浅島誠、向井秀夫、村上元、北条泰嗣、木本哲也、川戸佳
  • Journal Title: Neuroscientist 13(4) Pages: 323-334
  • Peer Reviewed
• [Journal Article] Rapid modulation of long-term depression and spinogenesis via synaptic estrogen receptors in hippocampal principal neurons (2007)
  • Author(s): 向井秀夫、釣木澤朋和、村上元、小南思郎、石井寛高、荻上(池田)真理、高田則雄、田辺伸聡、古川愛造、北条泰嗣、大石悠貴、Morrison J.H.、Janssen W. G.M.、Rose A. J.、Chambon P.、加藤茂明、泉俊輔、山崎岳、木本哲也、川戸佳
  • Journal Title: J. Neurochem. 100(4) Pages: 950-967
  • Peer Reviewed
• [Journal Article] Local production of sex hormones and their modulation of hippocampal synaptic plasticity (2007)
  • Author(s): 石井 寛高
  • Journal Title: Neuroscientist 13 Pages: 323-334
  • Peer Reviewed
• [Journal Article] Gonadotropin-Releasing Hormone Antagonist, Cetrorelix, Induces the Expression of Melatonin Receptor 1a (MT1) in the GnRH Neuronal Cell Line GT1-7
  • Author(s): 石井寛高、佐藤俊、尹成珠、佐久間康夫、加藤昌克
  • Journal Title: Neuroendocrinology (in press)
  • Peer Reviewed
• [Journal Article] Activation of A-type γ-amino butyric acid receptors (GABA_AR_s) excites gonadotropin-releasing hormone (GnRH) neurons isolated from adult rats
  • Author(s): 尹 成珠
  • Journal Title: Journal of Neuroendocrinology (in press)
  • Peer Reviewed
• [Presentation] ラットGnRHニューロンにおける電位依存性カルシウムチャネルの発現の発達段階における差異 (2008)
  • Author(s): 田中伸幸、石井寛高、尹成珠、加藤昌克、佐久間康夫
  • Organizer: 第35回日本神経内分泌学会・第23回日本下垂体学会合同学術集会
  • Place of Presentation: 政策研究大学院大学(六本木・東京)
  • Year and Date: 2008-08-30
• [Presentation] ラットメラトニン受容体の生化学的解析 (2008)
  • Author(s): 小林桃子、石井寛高、田中伸幸、加藤昌克、佐久間康夫
  • Organizer: 第35回日本神経内分泌学会・第23回日本下垂体学会合同学術集会
  • Place of Presentation: 政策研究大学院大学(六本木・東京)
  • Year and Date: 2008-08-30
• [Presentation] GnRHアンタゴニストのcetrorelixは、GT1-7細胞のメラトニン受容体1aの発現を誘導する (2008)
  • Author(s): 石井寛高、尹成珠、加藤昌克、佐久間康夫
  • Organizer: 第35回日本神経内分泌学会・第23回日本下垂体学会合同学術集会
  • Place of Presentation: 政策研究大学院大学(六本木・東京)
  • Year and Date: 2008-08-28
• [Presentation] Expression of voltage-gated Ca2+ channel mRNAs in GnRH neurons of male and female adult rats (2008)
  • Author(s): 田中伸幸、石井寛高、尹成珠、加藤昌克、佐久間康夫
  • Organizer: 第31回日本神経科学会大会
  • Place of Presentation: 、パシフィコ横浜(横浜・神奈川)
  • Year and Date: 2008-07-10
• [Presentation] Expression of voltage-gated Ca^<2+> channel mRNAs in GnRH neurons of male and female adult rats (2008)
  • Author(s): 田中伸幸
  • Organizer: 第31回日本神経科学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-07-10
• [Presentation] Neurosteroids synthesis in the rat hippocampus (2008)
  • Author(s): 木本哲也、肥後心平、石井寛高、村上元、川戸佳
  • Organizer: 第31回日本神経科学会大会
  • Place of Presentation: パシフィコ横浜(横浜・神奈川)
  • Year and Date: 2008-07-09
• [Presentation] Neurosteroids synthesis in the rat hinnocamnus (2008)
  • Author(s): 木本哲也
  • Organizer: 第31回日本神経科学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-07-09
• [Presentation] Neurosteroids synthesis in the rat hippocampus (2007)
  • Author(s): 木本哲也、肥後心平、北條泰嗣、石井寛高、川戸佳
  • Organizer: 第30回日本神経科学会大会
  • Place of Presentation: パシフィコ横浜(横浜・神奈川)
  • Year and Date: 2007-09-12
• [Presentation] Neurosteroids synthesis in the rat hippocampus (2007)
  • Author(s): 木本 哲也
  • Organizer: 第30回日本神経科学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-09-12
• [Presentation] Presence of gabazin-resistant GABAA receptor currents and their modulation by neurosteroids in rat GnRH(LHRH)neurons (2007)
  • Author(s): 尹成珠、田中伸幸、石井寛高、加藤昌克、佐久間康夫
  • Organizer: 第30回日本神経科学会大会
  • Place of Presentation: パシフィコ横浜(横浜・神奈川)
  • Year and Date: 2007-09-11
• [Presentation] Presence of gabazin-resistant GABA_A receptor currents and their modulation by neurosteroids in rat GnRH (LHRH) neurons (2007)
  • Author(s): 尹 成珠
  • Organizer: 第30回日本神経科学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-09-11