The relationship between vasopressin and circadian rhythm in the rat suprachiasmatic nucleus

• Project/Area Number: 19790183
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: FUJIHARA Hiroaki University of Occupational and Environmental Health, Japan, 医学部, 助教 (10369051)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: eGFP / 視交叉上核 / バゾプレッシン / サーカディアンリズム

Research Abstract

バゾプレッシン(AVP)-eGFPトランスジェニックラットの視交叉上核において遺伝子発現のサーカディアンリズムを測定し、時差ぼけ(位相変化)および光刺激に対する遺伝子発現の反応性を検討した。eGFP遺伝子発現はサーカディアンリズムを有しており、位相変化および光刺激に対して鋭敏に反応することを明らかにした。また、その反応性はAVP mRNAの変動より大きい可能性が示唆された。

Research Products

• [Journal Article] Response of arginine vasopressin-enhanced green fluorescent protein fusion gene in the hypothalamus of adjuvant-induced arthritic rats (2009)
  • Author(s): Suzuki H, Onaka T, Kasai M, Kawasaki M, Ohnishi H, Otsubo H, Saito T, Hashimoto H, Yokoyama T, Fujihara H, Dayanithi G, Murphy D, Nakamura T, Ueta Y
  • Journal Title: Journal of Neuroendocrinolog 21(3) Pages: 183-190
  • Peer Reviewed
• [Journal Article] Response of arginine vasopressin-enhanced green fluorescent protein fusion gene in the hypothalamus of adjuvant-induced arthritic rats (2009)
  • Author(s): Suzuki H, Fujihara H(14人中10番目)
  • Journal Title: Journal of Neuroendocrinolog 21(93) Pages: 183-190
  • Peer Reviewed
• [Journal Article] Brain-derived neurotrophic factor inhibits spontaneous inhibitory postsynaptic currents in the rat supraoptic nucleus. (2008)
  • Author(s): Ohbuchi T, Yokoyama T, Saito T, Hashimoto H, Suzuki H, Otsubo H, Fujihara H, Suzuki H, Ueta Y.
  • Journal Title: Brain Resarch 3 Pages: 34-42
  • Peer Reviewed
• [Journal Article] Specific expression of optically active reporter gene in arginine vasopressin-secreting neurosecretory cells in the hypothalamic- neurohypophyseal system. (2008)
  • Author(s): Ueta Y, Fujihara H, Dayanithi G, Kawata M, Murphy D.
  • Journal Title: Journal of Neuroendocrinology 20(6) Pages: 660-664
  • Peer Reviewed
• [Journal Article] Brain-derived neurotrophic factor inhibits spontaneous inhibitory postsynaptic currents in the rat supraoptic nucleus (2008)
  • Author(s): Ohbuchi T, Fujihara H(9人中7番目)
  • Journal Title: Brain Resarch 3 Pages: 34-42
  • Peer Reviewed
• [Journal Article] Specific expression of optically active reporter gene in arginine vasopressin-secreting neurosecretory cells in the hypothalamic-neurohypophyseal system (2008)
  • Author(s): Ueta Y, Fujihara H(5人中2番目)
  • Journal Title: Journal of Neuroendocrinology 20(6) Pages: 660-664
  • Peer Reviewed
• [Journal Article] Physiological studies of stress responses in the hypothalamus of vasopressin-enhanced green fluorescent protein transgenic rat. (2007)
  • Author(s): Shibata M, Fujihara H, Suzuki H, Ozawa H, Kawata M, Dayanithi G, Murphy D, Ueta Y.
  • Journal Title: Journal of Neuroendocrinology 19(4) Pages: 258-292
  • Peer Reviewed
• [Journal Article] Adrenomedullin 2 (AM2)/ intermedin is a more potent activator of hypothalamic oxytocin secreting neurons than AM possibly through an unidentified receptor in rats. (2007)
  • Author(s): Hashimoto H, Hyodo S, Kawasaki M, Shibata M, Saito T, Suzuki H, Otsubo H, Yokoyama T, Fujihara H, Higuchi T, Takei Y, Ueta Y.
  • Journal Title: Peptides 28(5) Pages: 1104-1112
  • Peer Reviewed
• [Journal Article] Centrally and peripherally administered ghrelin potently inhibits water intake in rats. (2007)
  • Author(s): Hashimoto H, Fujihara H, Kawasaki M, Saito T, Shibata M, Otsubo H, Takei Y, Ueta Y.
  • Journal Title: Endocrinology 148(4) Pages: 1638-1647
  • Peer Reviewed
• [Journal Article] Centrally and peripherally administered ghrelin photently inhibits water intake in rats. (2007)
  • Author(s): Hashimoto H
  • Journal Title: Endocrinology 148(4) Pages: 1638-1647
  • Peer Reviewed
• [Journal Article] Adrenomedullin 2 (AM2)/intermedin is a more potent activator of hypothalamicoxtccin-secreing neurons than AM Possibly through an unidentified receptor in rats. (2007)
  • Author(s): Hashimoto H.
  • Journal Title: Peptides 28(5) Pages: 1104-1112
  • Peer Reviewed
• [Journal Article] Physiological studies of stress responses in the hypothalamus of vasopressin-enhanced green fluorescent protein transgenic rat. (2007)
  • Author(s): Shibata M.
  • Journal Title: Journal of Neuroendocrinology 19(4) Pages: 285-292
  • Peer Reviewed
• [Presentation] バゾプレッシン-eGFPトランスジェニックラットの視交叉上核における遺伝子発現の概日リズム観察と光刺激に対する反応性の検討 (2008)
  • Author(s): 丸山崇、藤原広明
  • Organizer: 第35回日本神経内分泌学会
  • Place of Presentation: 東京・政策研究大学院大学
  • Year and Date: 2008-08-30
• [Presentation] 明期開始時刻の前進がトランスジェニックラットの視床下部におけるバゾプレッシン-eGPP融合遺伝子と時計遺伝子発現に及ぼす影響 (2008)
  • Author(s): 藤原広明
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京・京王プラザホテル
  • Year and Date: 2008-03-25
• [Presentation] AVP-eGFPトランスジェニックラットを用いた時差ぼけモデルにおける視床下部遺伝子発現の検討 (2008)
  • Author(s): 藤原広明
  • Organizer: 第18回日本病態生理学会
  • Place of Presentation: 神戸・神戸学院大学
  • Year and Date: 2008-01-26
• [Presentation] Effects of acute phase advance on the circadian gene expressions of galanin, arginine vasopressin-enhanced green fluorescent protein, Per 1 and Per 2 in the rat hypothalamus (2007)
  • Author(s): 藤原広明
  • Organizer: Neuroscience Meeting 2007
  • Place of Presentation: アメリカ合衆国カリフォルニア州サンディエゴ
  • Year and Date: 2007-11-07
• [Presentation] Effects of acute phase advance on the circadian gene expressions of. galanin, argininevasopressin-enhanced green fluorescent protein, Per 1 and Per 2 in the rat hypothalamus (2007)
  • Author(s): 藤原広明
  • Organizer: Neuroscience Meeting 2007
  • Place of Presentation: アメリカ合衆国カリフォルニア州サンディエゴ
  • Year and Date: 2007-11-07
• [Presentation] GFP monitoring in vitro in the posterior pituitary of arginine vasopressin-eGFP transgenic rat. (2007)
  • Author(s): 藤原広明
  • Organizer: Satellite Symposium of the 5th Congress of the International Society for Autonomic Neuroscience
  • Place of Presentation: 和歌山・ホテルグランヴィア
  • Year and Date: 2007-10-03
• [Presentation] GFP monitoring in vitro in the posterior pituitary arginine vasopressin -eGFP transenic rat. (2007)
  • Author(s): 藤原広明
  • Organizer: Satellite Symposium of the 5th Congress of the International Society for Autonmic Neuroscience
  • Place of Presentation: 和歌山・ホテルグランヴィア
  • Year and Date: 2007-10-03
• [Presentation] AVP-eGFP トランスジェニックラットを用いた時差ぼけモデルにおける視床下部遺伝子発現の検討 (2007)
  • Author(s): 藤原広明
  • Organizer: 第18回日本病態生理学会
  • Place of Presentation: 神戸・神戸学院大学
  • Year and Date: 2007-01-26