Repetitive injection of bone marrow-derived dendritic cells pulsed with α-galactosylceramide and peptide induce CD4 T cell tolerance

• Project/Area Number: 19790187
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General pharmacology
• Research Institution: Kyoto University
• Principal Investigator: IYODA Tomonori Kyoto University, 大学院・生命科学研究科, 助教 (60359784)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,720,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: 樹状細胞 / ナチュラルキラーT細胞 / 免疫寛容 / IL-10 / EAE / NKT細胞 / α-galactosylceramide / MOG / TGF-β1 / 制御性T細胞 / α-GalCer / 骨髄由来樹状細胞 / CD4陽性T細胞 / 血清サイトカイン

Research Abstract

α-ガラクドシルセラミド(α‐GC)とペプチド抗原を提示させた樹状細胞(DC)を1日おきに3回投与することでペプチド抗原特異的な免疫応答を抑制することが出来た。これはDCの頻回投与によりナチュラルキラーT細胞(NK細胞)のインターロイキン10(IL-10))産生が誘導され、CD4陽性T細胞のIL-10産生や腫瘍壊死因子β1の発現が上昇するためだと考えられる。また、DCによる免疫寛容の誘導にはNKT細胞とCD4陽性T細胞が同じDCにより抗原提示を受けることが必要であった。

Research Products

• [Journal Article] Dendritic cells derived from TBP-2-deficient mice are defective in inducing T cell responses (2008)
  • Author(s): Aoi Son, Hajime Nakamura, Tomonori Iyoda, Kayo Inaba and Junji Yodoi, et al.
  • Journal Title: Eur. J. Immunol. vol. 38 Pages: 1358-1367
  • Peer Reviewed
• [Journal Article] Dendritic cells derived from TBP-2-deficient mice are defective in inducing T cell responses (2008)
  • Author(s): Aoi Son, Hajime Nakamura, Tomonori Iyoda, Kayo Inaba and Junji Yodoi et a1.
  • Journal Title: Eur. J. Immunol. 38 Pages: 13581367-13581367
  • Peer Reviewed
• [Presentation] Anergy induction of N K T cells by dendritic cells presenting α-galactosylceramide (2009)
  • Author(s): 伊豫田智典
  • Organizer: The 5th International Symposium on CD1/NKT cells
  • Place of Presentation: 神奈川県
  • Year and Date: 2009-03-24
• [Presentation] Anergy induction of NKT cells by dendritic cells presenting α-galactosylceramide (2009)
  • Author(s): 伊豫田智典、稲葉カヨ, 他
  • Organizer: The 5th International Symposium on CD1/NKT cells
  • Place of Presentation: 神奈川県、鎌倉市
  • Year and Date: 2009-03-24
• [Presentation] Induction of anergy of NKT cells in response to a-galactosylceramide presented by dendritic cells (2007)
  • Author(s): 伊豫田智典
  • Organizer: 日本免疫学会総会・学術集会
  • Place of Presentation: 東京都
  • Year and Date: 2007-11-21
• [Presentation] Repetitive injection of dendritic cells pulsed with α-galactosylceramide effectively induce tolerance in an antigen-dependent and independent manner (2007)
  • Author(s): 羽床明子
  • Organizer: 日本免疫学会総会・学術集会
  • Place of Presentation: 東京都
  • Year and Date: 2007-11-21
• [Book] NKT細胞を介した樹状細胞によるIL-10産生T細胞の誘導 (2008)
  • Author(s): 伊豫田智典
• [Book] NKT細胞を介した樹状細胞によるIL-10産生T細胞の誘導 (2008)
  • Author(s): 伊豫田智典
  • Total Pages: 7
  • Publisher: 科学評論社