A study to elucidate the molecular mechanism of peptide neurotransmitter release

• Project/Area Number: 19790189
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General pharmacology
• Research Institution: Hiroshima University
• Principal Investigator: TANG He-Bin Hiroshima University, 大学院・医歯薬学総合研究科, 助教 (10403502)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,500,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: COX-1 / -2 / DRG neurons and non-neuronal cells / MAP kinases / NK-1R / the release and synthesis of SP / TRPV1 / the deletion of Nav1.8 / the intracellular Ca2+ signaling transduction / Dorsal root ganlion neurons / Nav1.8 / Neurokinin-1 receptor / PKC / SP release / TRPV 1 / Substande P / Dorsal root ganglion neurons / Dorsal root ganglion non-neuronal cells / Neurokinin-1 受容体 / Capsaicin / Ca^<2+>ion / Neuropeptide release

Research Abstract

末梢組織の損傷・炎症により知覚神経が活性化されると、知覚神経からsubstance P (SP)などの神経ペプチドが遊離され中枢へ痛みを伝えることが知られている。そのため、SPの動態を明らかにすることは疼痛発症メカニズムの解明に重要な役割を果たすと考えられる。本研究では、様々な侵害刺激による脊髄後根神経節(DRG)神経からのSP遊離についてラジオイムノアッセイ法を用いて検討し、さらにSP遊離を制御する分子メカニズムについて明らかにした。したがって、本研究により疼痛に対する新たな治療概念の構築に貢献する可能性が考えられる。

Research Products

• [Journal Article] Phosphorylation of TRPV1 byneurokinin-1 receptor agonist exaggerates the capsaicin-mediated substance Prelease from cultured rat dorsalroot ganglion neurons (2008)
  • Author(s): Tang HB, Li YS, Miyano K Nakata Y
  • Journal Title: Neuropharmacology 55 Pages: 1405-1411
  • Peer Reviewed
• [Journal Article] Involvement of Voltage-gated Sodium Channel NaV1.8 in the Regulation of the Release and Synthesis of Substance P in Adult Mouse Dorsal Root Ganglion Neurons. (2008)
  • Author(s): Tang HB, Shiba E, Li YS, Morioka N, Zheng TX, Ogata N Nakata Y
  • Journal Title: J Pharmacol Sci 108 Pages: 190-197
  • Peer Reviewed
• [Journal Article] The activation of transient receptor potential vanilloid receptor subtype 1 by capsaicin without extracellular Ca2+ is involved in bthe mechanism of distinct substance P release in cultured rat dorsal root ganglion neurons (2008)
  • Author(s): Tang HB and Nakata Y
  • Journal Title: Naunyn Schmiedebergs Arch Pharmacol 377 Pages: 325-332
  • Peer Reviewed
• [Journal Article] Phosphorylation of TRPV 1 by neurokinin-1 receptor agonist exaggerates the capsaicin-mediated substance Prelease from cultured rat dorsal root ganglion neurons (2008)
  • Author(s): Tang HB, Li YS, Miyano K, Nakata Y.
  • Journal Title: Neuropharmacology 55 Pages: 1405-1411
  • NAID: 120001092431
  • Peer Reviewed
• [Journal Article] Involvement of Voltage-gated Sodium Channel Nav1.8 in the Regulation of the Release and Synthesis of Substance P in Adult Mouse Dorsal Root Ganglion Neurons (2008)
  • Author(s): Tang HB, Shiba E, Li YS, Morioka N, Zheng TX, Ogata N, Nakata Y.
  • Journal Title: Journal of Pharmacological Sciences 108 Pages: 190-197
  • Peer Reviewed
• [Journal Article] The activation of transient receptoil potential vanilloid receptor subtype 1by capsaicin without extracellular Ca^<2+> is involved in the mechanism ofdistinctsubstance P release in cultured rat dorsal root ganglion neurons (2008)
  • Author(s): Tang HB, Nakata Y.
  • Journal Title: Naunyn-Schmiedeberg's Archives ofPharmacology (In press)
  • Peer Reviewed
• [Journal Article] The release of substance P fromcultured dorsal root ganglion neurons requires the non-neuronal cells around these neurons (2007)
  • Author(s): Tang HB, Li YS and Nakata Y
  • Journal Title: J Pharmacol Sci 105 Pages: 264-271
  • Peer Reviewed
• [Journal Article] Activation of the neurokinin-1 receptor by substance P triggers the release of substance P from cultured adult rat dorsal root ganglion neurons (2007)
  • Author(s): Tang HB, Li YS, Arihiro K and Nakata Y
  • Journal Title: Mol Pain 3 Pages: 42-42
  • Peer Reviewed
• [Journal Article] Activation of the neurokiniri-1 receptor by substance P triggers the releaseof substance P from cultured adult rat dorsal root ganglion neurons (2007)
  • Author(s): Tang HB, Li YS, Arihiro K, NakataY.
  • Journal Title: Molecular Pain 3
  • Peer Reviewed
• [Journal Article] The release of substance P from cultured dorsal root ganglion neurons requires the non-neuronal cells around these neurons (2007)
  • Author(s): Tang HB, Li YS, Nakata Y.
  • Journal Title: Journal of Pharmacological Sciences 105 Pages: 264-271
  • NAID: 10024316705
  • Peer Reviewed
• [Presentation] Elucidation of substance P release mechanisms (2008)
  • Author(s): Tang HB
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-19
• [Presentation] サブスタンスPの遊離機構の解明(Elucidation of substance P release mechanisms) (2008)
  • Author(s): 唐 和斌, 仲田 義勝(Tang HB, Nakata Y)
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-03-19
• [Presentation] DRGからの神経ペプチド(サブスタンスP)の遊離機構の解明 (2007)
  • Author(s): 唐和斌
  • Organizer: 第35回薬物活性シンポジウム
  • Place of Presentation: 広島
  • Year and Date: 2007-11-29
• [Presentation] DRG からの神経ペプチド(サブスタンスP)の遊離機構の解明 (2007)
  • Author(s): 唐 和斌、井上敦子、仲田義啓
  • Organizer: 第35回薬物活性シンポジウム
  • Place of Presentation: 広島
  • Year and Date: 2007-11-29