Purification and functional analysis of MafK complex

• Project/Area Number: 19790201
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Tohoku University
• Principal Investigator: KATOH Yasutake Tohoku University, 大学院・医学系研究科, 助教 (40397914)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,780,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 生体生命情報学 / タンパク質 / 発現制御 / プロテオーム / 転写因子 / プロテオミクス / MafK / 医化学 / タンパク質複合体

Research Abstract

本研究の主題は、転写因子MafKによる標的遺伝子の転写抑制化から活性化への制御機構を理解することにある。そこで、MafKによる転写制御に関与する因子の同定を目指して、MafK複合体を精製し、メチオニンアデノシル基転移酵素(MATII)を同定した。本研究は、核内に局在するMATIIがMafK標的遺伝子であるヘムオキシゲナーゼ1(HO-1)の転写を抑制的に制御することや、ゲノム全体のDNAやヒストンH3K4ないしH3K9のメチル化修飾を包括的に制御していることを明らかにした。

Research Products

• [Journal Article] Bach1 inhibits oxidative stress-induced cellular senescence by impeding p53 function on chromatin. (2008)
  • Author(s): Dohi Y, Ikura T, Hoshikawa Y, Katoh Y, Ota K, Nakanome A, Muto A, Omura S, Ohta T, Ito A, Yoshida M, Noda T, Igarashi K.
  • Journal Title: Nature structure and molecular biology 12 Pages: 1246-1254
  • Peer Reviewed
• [Journal Article] Bachl inhibits oxidative stress-induced cellular senescence by impeding p53 function on chromatin (2008)
  • Author(s): Dohi Y
  • Journal Title: Nature structure and molecular biology 12 Pages: 1246-1254
  • Peer Reviewed
• [Journal Article] Heme induces ubiquitination and degradation of the transcription factor Bach1. (2007)
  • Author(s): Zenke-Kawasaki Y, Dohi Y, Katoh Y, Ikura T, Ikura M, Asahara T, Tokunaga F, Iwai K, Igarashi K.
  • Journal Title: Molecular and cellular biology 27 Pages: 6962-6971
  • Peer Reviewed
• [Journal Article] Bach1 repression of ferritin and thioredoxin reductase1 is heme-sensitive in cells and in vitro and coordinates expression with heme oxygenase1, b-globin and NADP(H) quinone (oxido)reductase1. (2007)
  • Author(s): Hintze KJ, Katoh Y, Igarashi K, Theil EC
  • Journal Title: The Journal of biological chemistry 282 Pages: 34365-34371
  • Peer Reviewed
• [Journal Article] Bach1 repression of ferritin and thioredoxin reductase1 is heme-sensitive in cells and in vitro and coordinates expression with heme oxygenase1, β-globin and NADP (H) quinone (oxido) reductase1. (2007)
  • Author(s): Hintze KJ
  • Journal Title: The Journal of biological chemistry 282 Pages: 34365-34371
  • Peer Reviewed
• [Journal Article] Heme induces ubiquitination and degradation of the transcription factor Bach1. (2007)
  • Author(s): Zenke-Kawasaki Y
  • Journal Title: Molecular and cellular biology 27 Pages: 6962-6971
  • Peer Reviewed
• [Presentation] MafK複合体の精製と機能解析 (2009)
  • Author(s): 加藤 恭丈、井倉 毅、星川 裕、田代 聡、太田 嶺人、野田 哲生、五十嵐 和彦
  • Organizer: 遺伝情報DECODE・冬のワークショップ2009(口頭発表)
  • Place of Presentation: 越後湯沢
  • Year and Date: 2009-01-19
• [Presentation] MafK複合体の精製と機能解析 (2009)
  • Author(s): 加藤恭丈
  • Organizer: 遺伝情報DECODE・冬のワークショップ2009
  • Place of Presentation: 越後湯沢(湯沢グランドホテル)
  • Year and Date: 2009-01-19
• [Presentation] A novel function of MATII in MafK complex. (2009)
  • Author(s): Katoh Y, Ikura T, Hoshikawa Y, Tashiro S, Ohta M, Noda T, Igarashi K.
  • Organizer: JST International Symposium : Molecular mechanism of environmental response to food and oxygen III
  • Place of Presentation: Gonryo-kaikan in Tohoku University
• [Presentation] A novel function of MATII in MafK complex (2009)
  • Author(s): 加藤恭丈
  • Organizer: JST International Symposium : Molecular mechanism of environmental response to food and oxygen III
  • Place of Presentation: 仙台(東北大学・艮陵会館)
• [Presentation] Ras^<V12>による形質転換における転写因子Bach1の機能解析 (2008)
  • Author(s): 中目 亜矢子、太田 一成、加藤 恭丈、井倉 毅、志賀 清人、小林 俊光、五十嵐 和彦
  • Organizer: 第31回日本分子生物学会年会,第81回日本生化学会大会(ポスター発表)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] MafK蛋白質複合体に含まれるPARP-1の役割 (2008)
  • Author(s): 加藤 恭丈、井倉 毅、星川 裕、野田哲生、五十嵐 和彦
  • Organizer: 第31回日本分子生物学会年会,第81回日本生化学会大会(ポスター発表)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] Coupling of transcription repression and methionine metabolism on chromatin. (2008)
  • Author(s): Katoh Y, Ohta M, Ikura T, Igarashi K.
  • Organizer: 第31回日本分子生物学会年会,第81回日本生化学会大会(口頭発表)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] Coupling of transcription repression and methionine metabolism on chromatin (2008)
  • Author(s): 加藤恭丈
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会
  • Place of Presentation: 神戸(神戸国際会議場)
  • Year and Date: 2008-12-11
• [Presentation] MafK蛋白質複合体に含まれるPARP-1の役割 (2008)
  • Author(s): 加藤恭丈
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会
  • Place of Presentation: 神戸(神戸国際会議場)
  • Year and Date: 2008-12-11
• [Presentation] Ras^<v12>による形質転換における転写因子Bachlの機能解析 (2008)
  • Author(s): 中目亜矢子
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会
  • Place of Presentation: 神戸(神戸国際会議場)
  • Year and Date: 2008-12-11
• [Presentation] MafK couples transcription repression and methionine metabolism. (2008)
  • Author(s): Katoh Y, Ikura T, Igarashi K.
  • Organizer: The 21^<st> NAITO CONFERENCE on Nuclear Dynamics and RNA [I]
  • Place of Presentation: Yatsugatake Royal Hotel, Yamanashi, Japan
• [Presentation] Purification and functional analysis of MafK complex. (2008)
  • Author(s): Katoh Y, Ikura T, Ikura M, Dohi Y, Muto A, Hoshikawa Y, Tashiro S, Noda T, Igarashi K.
  • Organizer: The 21st NAITO CONFERENCE on Nuclear Dynamics and RNA [I]
  • Place of Presentation: Yatsugatake Royal Hotel, Yamanashi, Japan
• [Presentation] Purification and functional analysis of MaiK complex (2008)
  • Author(s): 加藤恭丈
  • Organizer: The 21^<st> NAITO CONFERENCE on Nuclear Dynamics and RNA [I]
  • Place of Presentation: 長野(八ヶ岳ロイヤルホテル)
• [Presentation] MafK couples transcription repression and methionine metabolism (2008)
  • Author(s): 加藤恭丈
  • Organizer: The 21^<st> NAITO CONFERENCE on Nuclear Dynamics and RNA [I]
  • Place of Presentation: 長野(八ヶ岳ロイヤルホテル)
• [Presentation] The function of MafK complex regulating target genes., MafK蛋白質複合体による遺伝子制御機構の解析 (2007)
  • Author(s): Katoh Y, Ikura T, Ikura M, Dohi Y, Muto A, Hoshikawa Y, Tashiro S, Noda T, Igarashi K.
  • Organizer: 第30回日本分子生物学会年会,第80回日本生化学会大会(ポスター発表)
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13
• [Presentation] MafK蛋白質複合体による遺伝子制御機構の解析 (2007)
  • Author(s): 加藤 恭丈
  • Organizer: 第30回日本分子生物学会年会, 第80回日本生化学会大会
  • Place of Presentation: 横浜(パシフィコ横浜)
  • Year and Date: 2007-12-12
• [Presentation] Purification of MafK omplex. (2007)
  • Author(s): Katoh Y, Ikura T, Ikura M, Dohi Y, Muto A, Hoshikawa Y, Tashiro S, Noda T, Igarashi K.
  • Organizer: JST-ERATO Yamamoto Environmental Response Project International Symposium : The Environmental Response
  • Place of Presentation: EPOCHAL Tsukuba International Congress Center, Japan
• [Presentation] Purification of MafK complex. (2007)
  • Author(s): 加藤 恭丈
  • Organizer: JST-ERATO Yamamoto Environmental Response Project International Symposium: The Environmental Response
  • Place of Presentation: 筑波(エポカルつくば)