Mechanisms of epithelial polarization by cell-cell and cell-matrix adhesion molecules

• Project/Area Number: 19790209
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Kobe University (2008); Osaka University (2007)
• Principal Investigator: IKEDA Wataru Kobe University, 大学院・医学研究科, 講師 (90362699)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: アドへレンスジャンクション / タイトジャンクション / ネクチン / カドヘリン / JAM / クローディン / 細胞極性 / アドヘレンスジャンクション

Research Abstract

上皮細胞にはアドへレンスジャンクション(AJ)とタイトジャンクション(TJ)という二つの主要な細胞間接着装置が存在し、必ずTJ はAJの頭頂側に位置する。これを上皮細胞の極性というが、その形成機構は明らかではない。本研究では、上皮細胞の極性形成機構の解明を目指し、細胞間接着分子を中心に解析を行った。その結果、TJ構成因子がTJの形成されるべき場所に集積するには、AJの接着分子ネクチンが重要であることを明らかにした。

Research Products

• [Journal Article] Roles of cell adhesion molecules nectin and nectin-like molecule-5 in the regulation of cell movement and proliferation (2008)
  • Author(s): Ogita H., Ikeda W., Takai Y.
  • Journal Title: J Microsc 231 Pages: 455-465
  • NAID: 120001384791
  • Peer Reviewed
• [Journal Article] Nectins and nectin-like molecules : roles in contact inhibition of cell movement and proliferation (2008)
  • Author(s): Takai Y., Miyoshi J., Ikeda W., Ogita H.
  • Journal Title: Nat Rev Mol Cell Biol 9 Pages: 603-615
  • Peer Reviewed
• [Journal Article] A novel role of nectin: implication in the co-localization of JAM-A and claudin-1 at the same cell-cell adhesion membrane domain (2008)
  • Author(s): Kuramitsu K., Ikeda W., Inoue N., Tamaru Y., Takai Y.
  • Journal Title: Genes Cells 13 Pages: 797-805
  • Peer Reviewed
• [Journal Article] Sequential activation of Rap1 and Rac1 small G proteins by PDGF locally at leading edges of NIH3T3 cells (2008)
  • Author(s): Takahashi M., Rikitake Y., Nagamatsu Y., Hara T., Ikeda W., Hirata K., Takai Y.
  • Journal Title: Genes Cells 13 Pages: 549-569
  • NAID: 120001384732
  • Peer Reviewed
• [Journal Article] Roles of Necl-5/poliovirus receptor and Rho-associated kinase (ROCK) in the regulation of transformation of integrin alphaVbeta3-based focal complexes into focal adhesions (2008)
  • Author(s): Nagamatsu Y., Rikitake Y., Takahashi M., Deki Y., Ikeda W., Hirata K., Takai Y.
  • Journal Title: J Biol Chem 283 Pages: 14532-14541
  • Peer Reviewed
• [Journal Article] Interaction and localization of Necl-5 and PDGF receptor β at the leading edges of moving NIH3T3 cells : implications for directional cell movement (2008)
  • Author(s): Amano H., Ikeda W., Kawano S., Kajita M., Tamaru Y., Inoue N., Minami Y., Yamada A., Takai Y.
  • Journal Title: Genes Cells 13 Pages: 269-284
  • NAID: 120001384731
  • Peer Reviewed
• [Journal Article] Establishment of cell polarity by afadin during the formation of embryoid bodies (2008)
  • Author(s): Komura H., Ogita H., Ikeda W., Mizoguchi A., Miyoshi J., Takai Y.
  • Journal Title: Genes Cells 13 Pages: 79-90
  • NAID: 120001384790
  • Peer Reviewed
• [Journal Article] Interaction of cancer cells with platelets mediated by Necl-5/poliovirus receptor enhances cancer cell metastasis to the lungs (2008)
  • Author(s): Morimoto K., Satoh-Yamaguchi K., Hamaguchi A., Inoue Y., Takeuchi M., Okada M., Ikeda W., Takai Y., Imai T.
  • Journal Title: Oncogene 27 Pages: 264-273
  • Peer Reviewed
• [Journal Article] Roles of cell adhesion molecules nectin and nectin-like molecule-5 in the regulation of cell movement and proliferation (2008)
  • Author(s): Ogita, H.
  • Journal Title: Journal of Microscopy 231 Pages: 455-465
  • Peer Reviewed
• [Journal Article] Nectins and nectin-like molecules : roles in contact inhibition of cell movement and proliferation (2008)
  • Author(s): Takai, Y.
  • Journal Title: Nature Review Molecular Cell Biology 9 Pages: 603-615
  • Peer Reviewed
• [Journal Article] Novel role of nectin : implication in the co-localization of JAM-A and claudin-l at the same cell-cell adhesion membrane domain (2008)
  • Author(s): Kuramitsu, K.
  • Journal Title: Genes to Cells 13 Pages: 797-805
  • Peer Reviewed
• [Journal Article] Sequential activation of Rapl and Racl small G proteins by PDGF locally at leading edges of NIH3T3 cells (2008)
  • Author(s): Takahashi, M.
  • Journal Title: Genes to Cells 13 Pages: 549-569
  • Peer Reviewed
• [Journal Article] Roles of Necl-5/poliovirus receptor and Rho-associated kinase (ROCK) in the regulation of transformation of integrin alpha Vbeta3-based focal complexes into focal adhesions (2008)
  • Author(s): Nagamatsu, Y
  • Journal Title: Journal of Biological Chemistry 283 Pages: 14532-14541
  • Peer Reviewed
• [Journal Article] Interaction of cancer cells with platelets mediated by Nec1-5/poliovirus receptor enhances cancer cell metastasis to the lungs. (2008)
  • Author(s): Morimoto, K.
  • Journal Title: Oncogene 27 Pages: 264-273
  • Peer Reviewed
• [Journal Article] Establishment of cell polarity by afadin during the formation of embryoid bodies. (2008)
  • Author(s): Komura, H.
  • Journal Title: Genes Cells 13 Pages: 79-90
  • Peer Reviewed
• [Journal Article] Interaction and localization of Nec1-5 and PDGF receptor βat the leadingedges of moving NIH3T3 cells: implications for directional cell movement. (2008)
  • Author(s): Amano, H.
  • Journal Title: Genes Cells In press
  • Peer Reviewed
• [Journal Article] The roles of nectins in cell adhesions : cooperation with other cell adhesion molecules and growth factor receptors (2007)
  • Author(s): Sakisaka T., Ikeda W., Ogita H., Fujita N., Takai Y.
  • Journal Title: Curr Opin Cell Biol 19 Pages: 593-602
  • Peer Reviewed
• [Journal Article] Necl-5/poliovirus receptor interacts in cis with integrin alphaVbeta3 and regulates its clustering and focal complex formation (2007)
  • Author(s): Minami Y., Ikeda W., Kajita M., Fujito T., Amano H., Tamaru Y., Kuramitsu K., Sakamoto Y., Monden M., Takai Y.
  • Journal Title: J Biol. Chem 282 Pages: 18481-18496
  • Peer Reviewed
• [Journal Article] Nec1-5/poliovirus receptor interacts in cis with integrin alpha Vbeta3 and regulates its clustering and focal complex formation. (2007)
  • Author(s): Minami, Y.
  • Journal Title: J. Biol. Chem. 282 Pages: 18481-18496
  • Peer Reviewed
• [Presentation] Regulatory Mechanism of Contact Inhibition of Cell Movement and Proliferation and Its Loss in Cancer Cells (2008)
  • Author(s): 池田わたる
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-10-29
• [Presentation] Necl-5/PVR as a Membrane Cue for Attracting Growing Microtubules to Leading Edges of Moving NIH3T3 Cells (2008)
  • Author(s): 岸本恵実
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-10-28
• [Presentation] Nec1-5/PVR as a Membrane Cue for Attracting Growing Microtubules to Leading Edges of Moving NIH3T3 Cells (2008)
  • Author(s): 岸本 恵実
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-10-28
• [Presentation] Necl-5とRhoキナーゼによるフォーカルコンプレックスからフォーカルアドヒ-ジョンへの移行の制御 (2007)
  • Author(s): 永松裕一
  • Organizer: 第30回日本分子生物学会・第80回日本生化学学会合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-12
• [Presentation] NH3T3細胞におけるPDGF刺激時の運動先導端へのRap1のリクルートによるRac1の活性化 (2007)
  • Author(s): 高橋玄倫
  • Organizer: 第30回日本分子生物学会・第80回日本生化学学会合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-12
• [Presentation] Necl-2によるインテグリンα6β4とErbB2の局在とシグナル伝達の制御 (2007)
  • Author(s): 河野智
  • Organizer: 第30回日本分子生物学会・第80回日本生化学学会合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-12
• [Presentation] Nec1-5とRhoキナーゼによるフォーカルコンプレックスからフォーカルアドヒージョンへの移行の制御 (2007)
  • Author(s): 永松 裕一
  • Organizer: 第30回日本分子生物学会・第80回日本生化学学会合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-12
• [Presentation] NH3T3細胞におけるPDGF刺激時の運動先導端へのRap1のリクルートによるRac1の活性化 (2007)
  • Author(s): 高橋 玄倫
  • Organizer: 第30回日本分子生物学会・第30回日本生化学学会合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-12
• [Presentation] Nec1-2によるインテグリンα6β4とErbB2の局在とシグナル伝達の制御 (2007)
  • Author(s): 河野 智
  • Organizer: 第30回日本分子生物学会・第80回日本生化学学会合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-12
• [Presentation] Formation of apico-basal polarity by two lg-like cell-cell adhesion molecules nectin and JAM (2007)
  • Author(s): 蔵満薫
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-10-04
• [Presentation] Formation of a ternary complex of Necl-5, PDGF receptor, and integrin αvβ3 at leading edges of moving cells (2007)
  • Author(s): 天野恭志
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-10-04
• [Presentation] Formation of a ternary complex of Nec1-5, PDGF receptor, and integrin αvβ3 at leading edges of moving cells (2007)
  • Author(s): 天野 恭志
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-10-03
• [Book] Annu Rev Cell Dev Biol (2008)
  • Author(s): Takai Y., Ikeda W., Ogita H., Rikitake Y.
  • Publisher: Annual Review
• [Book] Annual Review Cell Developmental Biology, 24巻 (2008)
  • Author(s): Takai, Y.
  • Publisher: Annual Review
• [Remarks] ホームページ
  • URL: http://www.med.kobe-u.ac.jp/mcb/
• [Remarks]
  • URL: http://wwt.med.kobe-u.ac.jp/mcb/