Functional Analysis of PSF1 and SLD5 in Stem Cell Division

• Project/Area Number: 19790210
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Osaka University
• Principal Investigator: UENO Masaya Osaka University, 微生物病研究所, 助教 (20334766)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,620,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: 幹細胞 / DNA複製 / DNA分配 / 遺伝子欠損マウス / バイオイメージング

Research Abstract

細胞が分裂するには、DNAが精緻に複製され、これが均等に分配される必要がある。本研究ではDNA複製因子であるPSF1およびSLD5が、染色体分配を制御し、個体の初期発生に必須であることを明らかとした。また、SLD5遺伝子が未分化細胞に発現することを利用し、癌組織中に存在する細胞分裂活性の高い特殊な癌細胞(癌幹細胞)の可視化を試みた。

Research Products

• [Journal Article] Identification and characterization of stem cell-specific transcription of PSF1 in spermatogenesis. (2009)
  • Author(s): Han Y, Ueno M, Nagahama Y, Takakura N.
  • Journal Title: Biochem Biophys Res Commun. 380 Pages: 609-613
  • Peer Reviewed
• [Journal Article] Both alleles of PSF1 are required for maintenance of pool size of immature bone marrow regeneration. (2009)
  • Author(s): Ueno M, Itoh M, Sugihara K, Asano M, Takakura N.
  • Journal Title: Blood. 13 Pages: 555-562
  • Peer Reviewed
• [Journal Article] Both alleles of PSF1 are required for maintenance of pool size of immature hematopoietic cells and acute bone marrow regeneration (2009)
  • Author(s): Ueno M
  • Journal Title: Blood 113 Pages: 555-562
  • Peer Reviewed
• [Journal Article] Spatial and temporal role of the apelin/APJ system in the caliber size regulation of blood vessels during angiogenesis. (2008)
  • Author(s): Kidoya H, Ueno M, Yamada Y, Mochizuki N, Nakata M, Yano T, Fujii R, Takakura N.
  • Journal Title: EMBO J. 27 Pages: 522-534
  • Peer Reviewed
• [Journal Article] Involvement of MDR1 function in proliferation of tumour cells. (2008)
  • Author(s): Katoh SY, Ueno M, Takakura N.
  • Journal Title: J Biochem. 143 Pages: 517-524
  • NAID: 10024909424
  • Peer Reviewed
• [Journal Article] Involvement of MDR1 function in proliferation of tumour cells (2008)
  • Author(s): Katoh SY
  • Journal Title: J Biochem 143 Pages: 517-524
  • Peer Reviewed
• [Journal Article] Spatial and temporal role of the apelin/APJ system in the caliber size regulation of blood vessels during angiogenesis (2008)
  • Author(s): Kidoya H
  • Journal Title: EMBO J 27 Pages: 522-534
  • Peer Reviewed
• [Journal Article] EphB4 overexpression in B16 melanoma cells affects arterial-venous patterning in tumor angiogenesis. (2007)
  • Author(s): Huang X, Yamada Y, Kidoya H, Naito H, Nagahama Y, Kong L, Katoh SY, Li WL, Ueno M, Takakura N.
  • Journal Title: Cancer Res. 67 Pages: 9800-9808
  • Peer Reviewed
• [Journal Article] Essential roles of the CC chemokine ligand 3-CC chemokine receptor 5 axis in bleomycin-induced pulmonary fibrosis through regulation of macrophage and fibrocyte infiltration. (2007)
  • Author(s): Ishida Y, Kimura A, Kondo T, Hayashi T, Ueno M, Takakura N, Matsushima K, Mukaida N.
  • Journal Title: Am J Pathol. 170 Pages: 843-854
  • Peer Reviewed
• [Journal Article] Essential roles of the CC chemokine ligand 3-CC chemokine receptor 5 axis in bleomycin-induced pulmonary fibrosis through regulation of macrophage and fibrocyte infiltration. (2007)
  • Author(s): Ishida Y.
  • Journal Title: Am J Pathol 170 Pages: 843-854
  • Peer Reviewed
• [Journal Article] EphB4 overexpression on B16 melanoma cells affects arterial-venous patterning in tumor angiogenesis. (2007)
  • Author(s): Huang X.
  • Journal Title: Cancer Res 67 Pages: 9800-9808
  • Peer Reviewed
• [Presentation] PSF1 is essential for bone marrow regeneration. (2007)
  • Author(s): Masaya Ueno.
  • Organizer: 36^<th> Annual Scientific Meeting of the ISEH - Society for Hematology and Stem Cells
  • Place of Presentation: ハンブルク(ドイツ)
• [Presentation] PSF1 is essential for bone marrow regeneration (2007)
  • Author(s): Masaya Ueno
  • Organizer: 36th Annual Scientific Meeting of the ISEH - Society for Hematology and Stem Cells
  • Place of Presentation: ドイツ、ハンブルク
• [Remarks]
  • URL: http://www.biken.osaka-u.ac.jp/act/act_takakura.php
• [Remarks]
  • URL: http://www.biken.osaka-u.ac.jp/act/act_takakura.php