Establishment of the method of A9 dopaminergic neuron differentiation using Lmx1a-GFP homologous recombinant ES cell and transplantation of the GFP+ cells.
| Project/Area Number |
19790220
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | St.Marianna University School of Medicine |
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Principal Investigator |
CHIBA Shunmei St.Marianna University School of Medicine, 大学院・医学研究科, 准教授 (20367361)
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| Project Period (FY) |
2007 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,140,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥540,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | 脳・神経 / 再生医学 / 胚性幹細胞 / パーキンソン病 / 相同性遺伝子導入 / 移植治療 |
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| Research Abstract |
To know the efficacy of A9 dopaminergic (DA) neuron differentiation from mouse ES cell, we established Lmx1a-GFP K/I ES cells by homologous recombination. Depend on the monitoring of GFP expression, we also established the efficient method to induce DA neurons from ES cells with appropriate concentration of noggin, Shh, and FGF8 factors. After transplantation of GFP positive cells, they survived and differentiated into A9 DA neurons in the striatum of Parkinsonian mice.
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Report
(6 results)
Research Products
(10 results)
