Establishment of the method of A9 dopaminergic neuron differentiation using Lmx1a-GFP homologous recombinant ES cell and transplantation of the GFP+ cells.

• Project/Area Number: 19790220
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: St.Marianna University School of Medicine
• Principal Investigator: CHIBA Shunmei St.Marianna University School of Medicine, 大学院・医学研究科, 准教授 (20367361)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,140,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥540,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: 脳・神経 / 再生医学 / 胚性幹細胞 / パーキンソン病 / 相同性遺伝子導入 / 移植治療

Research Abstract

To know the efficacy of A9 dopaminergic (DA) neuron differentiation from mouse ES cell, we established Lmx1a-GFP K/I ES cells by homologous recombination. Depend on the monitoring of GFP expression, we also established the efficient method to induce DA neurons from ES cells with appropriate concentration of noggin, Shh, and FGF8 factors. After transplantation of GFP positive cells, they survived and differentiated into A9 DA neurons in the striatum of Parkinsonian mice.

Research Products

• [Journal Article] SJLB mice develop tauopathy-induced parkinsonism. (2010)
  • Author(s): Takenokuchi M, Kadoyama K, Chiba S, Sumida M, Matsuyama S, Saigo K, Taniguchi T.
  • Journal Title: Neurosci Lett. 473 Pages: 182-185
  • Peer Reviewed
• [Journal Article] SJLB mice develop tauopathy-induced parkinsonism. (2010)
  • Author(s): Takenokuchi M, Kadoyama K, Chiba S, Sumida M, Matsuyama S, Saigo K, Taniguchi T.
  • Journal Title: Neurosci Lett. Volume: 473 Pages: 182-185
  • Peer Reviewed
• [Journal Article] Loss of Dopaminoreceptive Neurons causes L-dopa Resistant Parkinsonism in Tauopathy
  • Author(s): Shunmei Chiba, Erika Takada, Mamoru Tadokoro, Taizo Taniguchi, Keiichi Kadoyama, Noboru Suzuki, Seiya Kato
  • Journal Title: Neurobiology of Aging, resived
  • Peer Reviewed
• [Presentation] タウ型認知症における難治性パーキンソニズムの組織・病理学的検討 (2011)
  • Author(s): 千葉俊明、田所衛、鈴木登、加藤誠也
  • Organizer: 第100回日本病理学会
  • Place of Presentation: 横浜
• [Presentation] PARKINSONISM INDUCED BY TAUOPATHY : ANALYSIS OF SJLB MICE (2010)
  • Author(s): Taniguchi, T, Chiba, S.
  • Organizer: the 2010 ISN/APSN joint meeting
  • Place of Presentation: Busan, Korea
  • Year and Date: 2010-09-27
• [Presentation] 難治性パーキンソニズムを伴うタウ型認知症モデルマウスにおける組織・病理学的検討 (2010)
  • Author(s): 千葉俊明
  • Organizer: 第29回分子病理学研究会
  • Place of Presentation: 筑波大学 総合研究棟D115・116室
  • Year and Date: 2010-08-01
• [Presentation] 神経再生を中心とした再生医学・幹細胞研究 (2010)
  • Author(s): 千葉俊明
  • Organizer: 平成22年総会琉球大学医学会
  • Place of Presentation: 琉球大学臨床講義室
  • Year and Date: 2010-07-22
• [Presentation] Loss of Dopaminoreceptive Neurons causes L-dopa ResistantParkinsonism in Tauopathy. (2010)
  • Author(s): 千葉俊明、谷口泰造、田所衛、鈴木登、加藤誠也
  • Organizer: 第29回分子病理研究会
  • Place of Presentation: 筑波
• [Presentation] 認知症モデルマウスにおける移植治療への検討 (2010)
  • Author(s): 千葉俊明、谷口泰造、鈴木登
  • Organizer: 第9回日本再生医療学会
  • Place of Presentation: 広島
• [Patent(Industrial Property Rights)] 非ヒトモデル動物を用いたパーキンソン症候群の検査方法 (2009)
  • Inventor(s): 谷口泰造、千葉俊明
  • Industrial Property Rights Holder: 谷口泰造
  • Filing Date: 2009-08-21