The expression and function of CD133 in cancer cells

• Project/Area Number: 19790240
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pathological medical chemistry
• Research Institution: Kinki University
• Principal Investigator: ARAO Tokuzo Kinki University, 医学部, 講師 (20441074)
• Research Collaborator: NISHIO Kazuto 近畿大学, 医学部, 教授 (10208134)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,230,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: CD133 / がん幹細胞 / 分子腫瘍学 / mTORシグナル / HIF-1A / 消化器癌 / 幹細胞 / マイクロアレイ

Research Abstract

がん細胞の幹細胞マーカーCD133の発現は、mTORシグナル阻害により発現が誘導され、低酸素およびHIF-1A誘導剤により発現が著明に抑制されることを見出した.本研究によりCD133発現制御メカニズムは、HIF-1Aを介して抑制的に制御されていることを初めて明らかにした.この研究はがん幹細胞の発生初期のシグナル伝達およびがん幹細胞機能維持の原因解明につながる手がかりになると考えられる.

Research Products

• [Journal Article] Circulating endothelial cells in non-small cell lung cancer patients treated with carboplatin and paclitaxel (2009)
  • Author(s): Kawaishi M, Fujiwara Y, Fukui T, Kato T, Yamada K, Ohe Y, Kunitoh H, Sekine I, Yamamoto N, Nokihara H, Watabe T, Shimoda Y, Arao T, Nishio K, Tamura T, Koizumi F
  • Journal Title: J Thorac Oncol 4(2) Pages: 208-13
• [Journal Article] Epidermal growth factor receptor lacking C-terminal autophosphorylation sites retains signal transduction and high sensitivity to epidermal growth factor receptor tyrosine kinase inhibitor (2009)
  • Author(s): Maegawa M, Arao T, Yokote H, Matsumoto K, Kudo K, Tanaka K, Kaneda H, Fujita Y, Ito F, Nishio K
  • Journal Title: Cancer Sci 100(3) Pages: 552-7
  • NAID: 10025143753
• [Journal Article] Identification of Predictive Biomarkers for Response to Trastuzumab Using Plasma FUCA Activity and N-Glycan Identified by MALDI-TOF-MS (2009)
  • Author(s): Matsumoto K, Shimizu C, Arao T, Andoh M, Katsumata N, Kohno T, Yonemori K, Koizumi F, Yokote H, Aogi K, Tamura K, Nishio K, Fujiwara Y
  • Journal Title: J Proteome Res 8(2) Pages: 457-462
• [Journal Article] Identification of prognostic biomarkers in gastric cancer using endoscopic biopsy samples (2008)
  • Author(s): Yamada Y, Arao T, Gotoda T, Taniguchi H, Oda I, Shirao K, Shimada Y, Hamaguchi T, Kato K, Hamano T, Koizumi F, Tamura T, Saito D, Shimoda T, Saka M, Fukagawa T, Katai H, Sano T, Sasako M, Nishio K
  • Journal Title: Cancer Sci 99(11) Pages: 2193-9
  • NAID: 10024012959
• [Journal Article] Association of epidermal growth factor receptor (EGFR)gene mutations with EGFR amplification in advanced non-small cell lung cancer (2008)
  • Author(s): Morinaga R, Okamoto I, Fujita Y, Arao T, Sekijima M, Nishio K, Ito H, Fukuoka M, Kadota J, Nakagawa K
  • Journal Title: Cancer Sci 99(12) Pages: 2455-60
  • NAID: 10024014322
• [Journal Article] EphA4 promotes cell proliferation and migration through a novel EphA4-FGFR1 signaling pathway in the human glioma U251 cell line (2008)
  • Author(s): Fukai J, Yokote H, Yamanaka R, Arao T, Nishio K, Itakura T
  • Journal Title: Mol Cancer Ther 7(9) Pages: 2768-78
• [Journal Article] N-Glycan fucosylation of epidermal growth factor receptor modulates receptor activity and sensitivity to epidermal growth factor receptor tyrosine kinase inhibitor (2008)
  • Author(s): Matsumoto K, Yokote H, Arao T, Maegawa M, Tanaka K, Fujita Y, Shimizu C, Hanafusa T, Fujiwara Y, Nishio K
  • Journal Title: Cancer Sci 99(8) Pages: 1611-7
  • NAID: 10024009907
• [Journal Article] Establishment and molecular profiling of a novel human pancreatic cancer panel for 5-FU (2008)
  • Author(s): Yanagihara K, Takigahira M, Tanaka H, Arao T, Aoyagi Y, Oda T, Ochiai A, Nishio K
  • Journal Title: Cancer Sci 99(9) Pages: 1859-64
  • NAID: 10024011346
• [Journal Article] Aberrant expression of Fra-2 promotes CCR4 expression and cell proliferation in adult T-cell leukemia (2008)
  • Author(s): Nakayama T, Hieshima K, Arao T, Jin Z, Nagakubo D, Shirakawa AK, Yamada Y, Fujii M, Oiso N, Kawada A, Nishio K, Yoshie O
  • Journal Title: Oncogene 27(23) Pages: 3221-32
• [Journal Article] Clock and ATF4 transcription system regulates drug resistance in human cancer cell lines. (2007)
  • Author(s): Igarashi Y
  • Journal Title: Oncogene 26(33) Pages: 4749-4760
  • Peer Reviewed
• [Journal Article] ZNF143 interacts with p73 and is involved in cisplatin resistance. (2007)
  • Author(s): Wakasugi
  • Journal Title: Oncogene 26(36) Pages: 5194-5203
  • Peer Reviewed
• [Journal Article] AZD2171 shows potent antitumor activity against gastric cancer over-expressing FGFR2/KGFR (2007)
  • Author(s): Takeda M
  • Journal Title: Clin Cancer Res. 13(10) Pages: 3051-3057
  • Peer Reviewed
• [Journal Article] Antibody-dependent cellular cytotoxicity of cetuximab against tumor cells with wild-type of mutant epidermal growth factor receptor. (2007)
  • Author(s): Kimura H
  • Journal Title: Cancer Sci. 98(8) Pages: 1275-1280
  • Peer Reviewed
• [Journal Article] Aberrant expression of Fra-2 promotes CCR4 expression and cell proliferation in adult T-cell leukemia.
  • Author(s): Nakayama T
  • Journal Title: Oncogene Epub
  • Peer Reviewed
• [Presentation] Biomarker Discovery for Angiogenesis with Multi-Plex Immunoassay (2008)
  • Author(s): 荒尾徳三
  • Organizer: 第67回日本癌学会学術総会ランチョンセミナー31
  • Place of Presentation: 名古屋
• [Presentation] Multi-Plex Immunoassay(Bio-Plex) (2008)
  • Author(s): 西尾和人
  • Organizer: 第46回日本癌治療学会総会シンポジウム
  • Place of Presentation: 名古屋
• [Presentation] Microarray analysis for gastrointestinal cancer (2008)
  • Author(s): 山田康秀
  • Organizer: 第67回日本癌学会学術総会ワークショップ14-3
  • Place of Presentation: 名古屋
• [Presentation] Microarray analysis for EGFR tyrosine kinase inhibitor resistant cell line. (2008)
  • Author(s): Arao T
  • Organizer: American Association for Cancer Research Annual Meeting (AACR)
  • Place of Presentation: San Diego
• [Presentation] Suppression of CD133 expression by siRNA modulated the expression of the genes associated with differentiation in cancer cells (2008)
  • Author(s): Matsumoto K
  • Organizer: AACR
  • Place of Presentation: San Diego
• [Presentation] IMP-3 promotes cellular proliferation activity in gastric cancer (2008)
  • Author(s): Tanaka K
  • Organizer: AACR
  • Place of Presentation: San Diego
• [Presentation] Antitumor activity and mode of action of a unique interlocked molecule rotaxane (2008)
  • Author(s): Fujita Y
  • Organizer: AACR
  • Place of Presentation: San Diego
• [Presentation] Cells expressing EGFR lacing C-terminal are still hypersensitive to EGFR tyrosine kinase inhibitor (2008)
  • Author(s): Maegawa M
  • Organizer: AACR
  • Place of Presentation: San Diego
• [Presentation] FOXQ1 is a novel p21 regulator in colorectal cancer cells (2008)
  • Author(s): Kaneda H
  • Organizer: AACR
  • Place of Presentation: San Diego
• [Presentation] Microarray analysis for gastrointestinal cancer (2008)
  • Author(s): 山田康秀
  • Organizer: 第67回日本癌学会学術総会ワークショップ14-3
  • Place of Presentation: 名古屋市
• [Presentation] Inhibition of phosphor-tyrosine in VEGFR2+CD45dim population as a biomarker for VEGFE2 tyrosin kinase inhibitors (2008)
  • Author(s): Kudo K.
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋市
• [Presentation] Mass spectrometric analysis of plasma N-glycan in pancreas cancer (2008)
  • Author(s): Sakamoto H.
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋市
• [Presentation] Identification of predictive biomarkers for response to Trastuzumab using glycobiological analysis (2008)
  • Author(s): Matsumoto K
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋市
• [Presentation] Microarray analysis for wild type and delE746_A750 type of EGFR introduced cells (2008)
  • Author(s): Maegawa M.
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋市
• [Presentation] Antitumor activity and mode of action of a unique interlocked molecule rotaxane (2008)
  • Author(s): Fujita Y.
  • Organizer: American Association for Cancer Research Annual Meeting 2008
  • Place of Presentation: San Diego
• [Presentation] uppression of CD133 expression by siRNA modulated the expresskon of the genes associated with differentiationin cancer cell (2008)
  • Author(s): Matsumoto K.
  • Organizer: American Association for Cancer Research Annual Meeting 2008
  • Place of Presentation: San Diego
• [Presentation] IMP-3 promotes cellular proliferation activity in gastric cancer (2008)
  • Author(s): Tanaka K.
  • Organizer: American Association for Cancer Research Annual Meeting 2008
  • Place of Presentation: San Diego
• [Presentation] Microarray analysis for EGFR tyrosine kinase inhibitor resistant cell line (2008)
  • Author(s): Arao T.
  • Organizer: American Association for Cancer Research Annual Meeting 2008
  • Place of Presentation: San Diego
• [Presentation] Cells expressing EGFR lacing C-terminal are still hypersensitive to EGFR tyrosine kinase inhibitor (2008)
  • Author(s): Maegawa M.
  • Organizer: American Association for Cancer Research Annual Meeting 2008
  • Place of Presentation: San Diego
• [Presentation] FOXQ1 is a novel p21 regulator in colorectal cancer cells (2008)
  • Author(s): Kaneda H.
  • Organizer: American Association for Cancer Research Annual Meeting 2008
  • Place of Presentation: San Diego
• [Presentation] 胃癌高発現遺伝子KIAA1199の生物学的検討 (2007)
  • Author(s): 荒尾徳三
  • Organizer: 第11回がん分子標的治療研究会総会・ポスター
  • Place of Presentation: 大阪市
• [Presentation] Microdissection and extraction of RNA from cancer and stromal tissues in epithelial ovarian cancer. (2007)
  • Author(s): Tsuda H
  • Organizer: 第66回日本癌学会学術総会・ポスター
  • Place of Presentation: 横浜市
• [Presentation] Biomarker study for gastrointestinal cancer using microarray analysis. (2007)
  • Author(s): Arao T
  • Organizer: 第66回日本癌学会学術総会・シンポジウム
  • Place of Presentation: 横浜市
• [Presentation] Suppression of SRPX2 mRNA expression inhibits cellular growth and adhesion in gastric cancer cells. (2007)
  • Author(s): Tanaka K
  • Organizer: 第66回日本癌学会学術総会・ポスター
  • Place of Presentation: 横浜市
• [Presentation] KIAA1199 is a novel therapeutic target for gastric cancer (2007)
  • Author(s): Arao T
  • Organizer: American Association for Cancer Research, Annual Meeting
  • Place of Presentation: Los Angeles, CA
• [Presentation] SRPX2 is a novel therapeutic target in gastric cancer (2007)
  • Author(s): Tanaka K
  • Organizer: American Association for Cancer Research, Annual Meeting
  • Place of Presentation: Los Angeles, CA
• [Book] Cancer Treatment Navigator (2008)
  • Author(s): 荒尾徳三 松本和子 西尾和人
  • Publisher: メディカルレビュー社
• [Book] 日本臨床増刊号・肺癌 (2008)
  • Author(s): 荒尾徳三 金田裕靖 西尾和人
  • Publisher: 日本臨床社
• [Book] 日本臨床増刊号・肺癌 (2008)
  • Author(s): 木村英晴 荒尾徳三
  • Publisher: 日本臨床社
• [Book] がんの分子標的治療 (2008)
  • Author(s): 荒尾徳三 山田康秀 西尾和人
  • Publisher: 南山堂
• [Book] Cancer Treatment Navigator (2008)
  • Author(s): 荒尾徳三
  • Publisher: メディカルレビュー社
• [Book] がんの分子標的治療第45章消化器がん (2008)
  • Author(s): 荒尾徳三
  • Publisher: 南山堂
• [Book] 日本臨床増刊号・肺癌 (2008)
  • Author(s): 荒尾徳三
  • Publisher: 日本臨床社
• [Book] がん分子標的治療 (2007)
  • Author(s): 荒尾徳三
  • Publisher: メディカルレビュー社
• [Book] 最新医学 (2007)
  • Author(s): 西尾和人
  • Publisher: 最新医学社
• [Book] Mebio Oncology (2007)
  • Author(s): 荒尾徳三
  • Publisher: メジカルビュー社
• [Book] 分子消化器病 (2007)
  • Author(s): 荒尾徳三
  • Publisher: 先端医学社
• [Book] 細胞工学 (2007)
  • Author(s): 荒尾徳三
  • Publisher: 秀潤社
• [Book] 腫瘍内科 (2007)
  • Author(s): 荒尾徳三
  • Publisher: 科学評論社
• [Book] ゲノム医学 (2007)
  • Author(s): 阿部譲
  • Publisher: メディカルレビュー社
• [Remarks]
  • URL: http://www.med.kindai.ac.jp/genom/
• [Remarks]
  • URL: http://www.med.kindai.ac.jp/genom/arao.html
• [Remarks]
  • URL: http://www.med.kindai.ac.jp/genom/
• [Patent(Industrial Property Rights)] 胃癌の判定方法 (2008)
  • Inventor(s): 荒尾徳三、西尾和人他
  • Industrial Property Rights Holder: 近畿大学他
  • Industrial Property Number: 2008-126457
  • Filing Date: 2008-05-13
• [Patent(Industrial Property Rights)] N結合糖鎖を利用した膵臓癌の診断方法 (2008)
  • Inventor(s): 荒尾徳三、西尾和人他
  • Industrial Property Rights Holder: 近畿大学他
  • Industrial Property Number: 2008-123391
  • Filing Date: 2008-05-09
• [Patent(Industrial Property Rights)] 胃癌の判定方法 (2008)
  • Inventor(s): 荒尾徳三, 他
  • Industrial Property Rights Holder: 近畿大学, 他
  • Industrial Property Number: 2008-126457
  • Filing Date: 2008-05-13
• [Patent(Industrial Property Rights)] N結合糖鎖を利用した膵臓癌の診断方法 (2008)
  • Inventor(s): 荒尾徳三, 他
  • Industrial Property Rights Holder: 近畿大学, 他
  • Industrial Property Number: 2008-123391
  • Filing Date: 2008-05-09