Analysis of regulation of Rab27A responsible for human inheritance disease Griscelli syndrome.

• Project/Area Number: 19790242
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Pathological medical chemistry
• Research Institution: Tohoku University
• Principal Investigator: ITOH Takashi Tohoku University, 大学院・生命科学研究科, 助教 (50373270)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,780,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 分子病態学 / 色素細胞 / 色素小胞 / Rab27A / メラノサイト / メラノソーム / リソソーム関連オルガネラ / FLJ13130 / mFLJ00332

Research Abstract

Griscelli症候群原因遺伝子であるRab27AはEPI64と呼ばれる酵素によって不活性化される。今回、EPI64を含むTBC1D10ファミリー (EPI64, FLJ13130, mFLJ00332) の機能の比較を行うことで、FLJ13130とmFLJ00332がEPI64とは異なる機能を持つことを明らかにでき、本申請の目的であった、EPI64の制御メカニズム解明の基礎的なデータを得ることが出来た。

Research Products

• [Journal Article] Identification and characterization of a novel Tre-2/Bub2/Cdc16 (TBC) protein that possesses Rab3A-GAP activity (2009)
  • Author(s): Ishibashi, K., Kanno, E., Itoh, T., and Fukuda, M.
  • Journal Title: Genes Cells 14(1) Pages: 41-52
  • Peer Reviewed
• [Journal Article] Identification and characterization of a novel Tre-2/Bub2/Cdc16 (TBC) protein that possesses Rab3A-GAP activity (2009)
  • Author(s): Ishibashi, K., 他
  • Journal Title: Genes to Cells 14(1) Pages: 41-52
  • Peer Reviewed
• [Journal Article] 低分子量GTPase Rabとエフェクター (2008)
  • Author(s): 伊藤敬、福田光則
  • Journal Title: 蛋白質 核酸 酵素 53(16) Pages: 2065-2070
• [Journal Article] Rab35 and its GAP EPI64C in T cells regulate receptor recycling and immunological synapse formation (2008)
  • Author(s): Patino-Lopez, G., Dong, X., Ben-Aissa, K., Bernot, K. M., Itoh, T., Fukuda, M., Kruhlak, M. J., Samelson, L. E., and Shaw, S.
  • Journal Title: J. Biol. Chem. 283(26) Pages: 18323-18330
  • Peer Reviewed
• [Journal Article] Rab35 and its GAP EPI64C in T cells regulate receptor recycling and immunologicalsynapse formation (2008)
  • Author(s): Patino-Lopez, G., 他
  • Journal Title: Journal of Biological Chemistry 283(26) Pages: 18323-18330
  • Peer Reviewed
• [Journal Article] 低分子量GTPase Rabとエフェクター (2008)
  • Author(s): 伊藤敬、福田光則
  • Journal Title: 蛋白質核酸酵素 53(16) Pages: 2065-2070
• [Presentation] TBCドメインを含む新規Rab3A-GAPのスクリーニング (2007)
  • Author(s): 石橋弘太郎、菅野栄子、伊藤敬、福田光則
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-13