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Studies on development of serodiagnosis system for dog infected in Echinococcus multilocularis by using specific recombinant antigen

Research Project

Project/Area Number 19790310
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Parasitology (including Sanitary zoology)
Research InstitutionHokkaido Institute of Public Health

Principal Investigator

KOUGUCHI Hirokazu  Hokkaido Institute of Public Health, 感染症センター生物科学部, 研究職員 (50435567)

Research Collaborator YAGI Kinpei  北海道立衛生研究所, 感染症センター, 主任研究員 (70414323)
KATOH Yoshinobu  北海道立衛生研究所, 感染症センター生物科学部, 主任研究員 (00414326)
MATSUMOTO Jun  日本大学, 生物資源学部・獣医学科, 専任講師 (70296169)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥2,430,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywords蛋白質 / 遺伝子 / バイオテクノロジー / エキノコックス / 寄生虫 / 組換え抗原 / 血清診断 / タンパク質
Research Abstract

エキノコックス症に感染したイヌの血清診断法に関する研究を行った。それまで、イヌの血清診断は全く未開発であったが、本研究により、診断法の基盤となる抗原をコードするいくつかの遺伝子や糖タンパク質成分を見いだすことが出来た。2年間の研究期間中、その遺伝子群を基に10数種の組換え抗原タンパク質を作製し、感染イヌおよび健常イヌ血清を用いて評価を行った。その中、数種類の感度および特異性共に良好な組換えタンパク質の作製に成功した。また、幼虫から抽出した糖タンパク質成分の血清学的評価も良好であった。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (16 results)

All 2009 2008 2007

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (6 results)

  • [Journal Article] Molecular characterization of the protease from Clostridium botulinum serotype Cresponsible for nicking in botulinum neurotoxin complex2009

    • Author(s)
      Suzuki T, Yoneyama T, Miyata K, Mikami A, Chikai T, Inui K, Kouguchi H, Niwa K, Watanabe T, Miyazaki S, Ohyama T
    • Journal Title

      Biochem Biophys Res Commun 379

      Pages: 309-313

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression and stability of the nontoxic component of the botulinum toxin complex2009

    • Author(s)
      Miyata K, Yoneyama T, Suzuki T, Kouguchi H, Inui K, Niwa K, Watanabe T, Ohyama T
    • Journal Title

      Biochem Biophys Res Commun 384

      Pages: 126-130

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization of the protease from Clostridium botulinum serotype C responsible for nicking in botulinum neurotoxin complex2009

    • Author(s)
      Tomonori Suzuki, et al.
    • Journal Title

      Biochemical and Biophysical Research Communications 379

      Pages: 309-312

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression and stability of the nontoxic component of the botulinum toxin complex2009

    • Author(s)
      Keita Miyata, et al.
    • Journal Title

      Biochemical and Biophysical Research Communications (印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of emY162 encoding an immunogenic protein cloned from an adult worm-specific cDNA library of Echinococcus multilocularis2008

    • Author(s)
      Yoshinobu Katoh, Hirokazu Kouguchi, Jun Matsumoto, Akiko Goto, Tomohiro Suzuki, Yuzaburo Oku and Kinpei Yagi
    • Journal Title

      Biochim Biophys Acta 1780

      Pages: 1-6

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 二次元電気泳動法を利用した多包虫症血清診断の試み2008

    • Author(s)
      孝口裕一 山野公明 加藤芳伸 鈴木智宏 八木欣平
    • Journal Title

      北海道立衛生研究所報 58

      Pages: 87-90

    • NAID

      120006794460

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 二次元電気泳動法を利用した多包虫症血清診断の試み2008

    • Author(s)
      孝口 裕一, ら
    • Journal Title

      北海道立衛生研究所報 58

      Pages: 87-90

    • NAID

      120006794460

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of emY162 encoding an immunogenic protein cloned from an adult worm-specific cDNA library of Echinococcus multilocularis2008

    • Author(s)
      Yoshinobu Katoh, et. al.
    • Journal Title

      Biochimica et Biophysica Acta 1780

      Pages: 1-6

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The vaccination potential of EMY162 antigen against Echinococcus multilocularis infection2007

    • Author(s)
      Hirokazu Kouguchi, Jun Matsumoto, Yoshinobu Katoh, Yuzaburo Oku, Tomohiro Suzuki and Kinpei Yagi
    • Journal Title

      Biochem Biophys Res Commun 363

      Pages: 915-920

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Vaccination Potential of EMY162 Antigen against Echinococcus multilocularis Infection2007

    • Author(s)
      Hirokazu Kouguchi, et. al.
    • Journal Title

      Biochemical and Biophysical Research Communications 363

      Pages: 915-920

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] EMY162 protein as a vaccine candidate to reduce level of alveolar hydatid disease2009

    • Author(s)
      Katoh Y, Kouguchi H, Matsumoto J, Suzuki T, Yagi K
    • Organizer
      World Conference of Magic Bullets (EHRLICH II) congress of hydatidology
    • Place of Presentation
      Nuremberg, Germany
    • Year and Date
      2009-10-03
    • Related Report
      2008 Final Research Report
  • [Presentation] 多包条虫etacestodeに対するワクチン開発に関する研究2009

    • Author(s)
      党志勝 八木欣平 奥祐三郎 孝口裕一 梶野喜一 渡辺純一 中尾亮 杉本千尋
    • Organizer
      日本獣医学会
    • Place of Presentation
      栃木県宇都宮市
    • Year and Date
      2009-04-03
    • Related Report
      2008 Final Research Report
  • [Presentation] 多包条虫metacestodeに対するワクチン開発に関する研究2009

    • Author(s)
      党 志勝, ら
    • Organizer
      第147回日本獣医学会学術集会
    • Place of Presentation
      栃木県宇都宮市栃木県総合文化センター
    • Year and Date
      2009-04-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] EMY162 protein as a vaccine candidate to reduce level of alveolar hydatid disease.2008

    • Author(s)
      Yoshinobu Katoh, et al.
    • Organizer
      World Conference of Magic Bullets(EHRLICH II)congress of hydatidology
    • Place of Presentation
      Nuremberg' Germany
    • Year and Date
      2008-10-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] 組換えEMY162の中間宿主における多包条虫感染防御効果2008

    • Author(s)
      孝口裕一 松本淳 加藤芳伸 奥祐三郎 鈴木智宏 八木欣平
    • Organizer
      日本獣医学会
    • Place of Presentation
      神奈川県相模原市
    • Year and Date
      2008-03-29
    • Related Report
      2008 Final Research Report
  • [Presentation] 組換えEMY162抗原の中間宿主における多包条虫感染防御効果2008

    • Author(s)
      八木 欣平
    • Organizer
      日本獣医学会
    • Place of Presentation
      神奈川県相模原市
    • Year and Date
      2008-03-29
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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