Investigation of the activation mechanism of the β-catenin signal by Helicobacter pylori CagA

• Project/Area Number: 19790312
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: Hokkaido University
• Principal Investigator: KAMIYA Naoko Hokkaido University, 遺伝子病制御研究所, 助教 (40279352)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,780,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 病原性 / ヘリコバクター・ピロリ / 胃癌 / CagA遺伝子 / β-cateninシグナル

Research Abstract

cagA遺伝子を保有するヘリコバクター・ピロリ菌(ピロリ菌)感染は、胃癌発症の危険率を有意に高める。これまでに、CagAがss-cateninシグナルを活性化することを報告している。本研究では、CagAが結合する新規細胞内標的分子を明らかにし、ss-cateninシグナルを活性化する分子機構を解明した。また、ss-cateninシグナル活性化に必要なCagAの責任領域を明らかにした。

Research Products

• [Journal Article] Structural and functional diversity in the PAR1b/MARK2-binding region of Helicobacter pylori CagA. (2008)
  • Author(s): H. Lu, Y. Saito, M. Umeda, N. Murata-Kamiya, H. Zhang, H. Higashi and M. Hatakeyama.
  • Journal Title: Cancer Sci. 99 Pages: 2004-2011
  • Peer Reviewed
• [Journal Article] ヘリコバクター・ピロリCagAによる細胞がん化メカニズム (2008)
  • Author(s): 齊藤康弘,紙谷尚子,畠山昌則
  • Journal Title: 実験医学 26 Pages: 2445-2452
• [Journal Article] 胃がん発症におけるelicobacter pylori CagAの意義 (2008)
  • Author(s): 齊藤康弘,紙谷尚子,畠山昌則
  • Journal Title: 血液・腫瘍科 56 Pages: 219-225
• [Journal Article] Y. Kurashima, N. Murata-Kamiya, K. Kikuchi, H. Higashi, T. Azuma, S. Kondo and M. Hatakeyama. (2008)
  • Author(s): Y. Kurashima, N. Murata-Kamiya, K. Kikuchi, H. Higashi, T. Azuma, S. Kondo and M. Hatakeyama.
  • Journal Title: Int. J. Cancer 122 Pages: 823-831
  • Peer Reviewed
• [Journal Article] Structural and functional diversity in the PARIb/MARK2-binding region of Helicobacter pylori CagA (2008)
  • Author(s): Lu, H
  • Journal Title: Cancer Science 99 Pages: 2004-2011
  • Peer Reviewed
• [Journal Article] Deregulation of β-catenin signal by Helicobacter pylori CagA requires the Ca2A-multimerization sequence (2008)
  • Author(s): Ktirashima, Y
  • Journal Title: International Journal of Cancer 122 Pages: 823-831
  • Peer Reviewed
• [Journal Article] 胃がん発症におけるHelicobacter pylori CagAの意義 (2008)
  • Author(s): 齊藤康弘
  • Journal Title: 血液・腫瘍科 56 Pages: 219-225
• [Journal Article] 胃がん発症におけるHelicobacter pylori CagAの意義。 (2008)
  • Author(s): 齊藤 康弘
  • Journal Title: 血液・腫瘍科 56 Pages: 219-225
• [Journal Article] Helicobacter pylori CagAはPAR1b/MARK2キナーゼを標的として上皮細胞の極性を破壊する (2007)
  • Author(s): 紙谷尚子,畠山昌則
  • Journal Title: 分子消化器病 4 Pages: 392-396
• [Journal Article] Helicobacter pylori CagA interacts with E-cadherin and deregulates the ss-catenin signal that promotes intestinal transdifferentiation in gastric epithelial cells. (2007)
  • Author(s): N. Murata-Kamiya, Y. Kurashima, Y. Teishikata, Y. Yamahashi, Y. Saito, H. Higashi, H. Aburatani, T. Akiyama, R.M. Peek Jr., T. Azuma and M. Hatakeyama.
  • Journal Title: Oncogane 26 Pages: 4617-4626
  • Peer Reviewed
• [Journal Article] Helicobacter pylori CagA targets PAR1/MARK kinase to disrupt epithelial polarity. (2007)
  • Author(s): I. Saadat, H. Higashi, C. Obuse, M. Umeda, N. Murata-Kamiya, Y. Saito, H. Lu, N. Ohnishi, T. Azuma, A. Suzuki, S. Ohno and M. Hatakeyama.
  • Journal Title: Nature 447 Pages: 330-333
  • Peer Reviewed
• [Journal Article] Deregulation of β -catenin signal by Helicobacter pylori CagA requires the CagA-multimerization sequence. (2007)
  • Author(s): Kurashima, Y.
  • Journal Title: International Journal of Cancer 122 Pages: 823-831
  • Peer Reviewed
• [Journal Article] Helicobacter pylori CagA targets PAR1/MARK kinase to disrupt epithelial cell polarity. (2007)
  • Author(s): Saadat, I.
  • Journal Title: Nature 447 Pages: 330-333
  • Peer Reviewed
• [Journal Article] Helicobacter pylori CagA interacts with E-cadherin and deregulates the β-catenin signal that promotes intestinal transdifferentiation in gastric epithelial cells. (2007)
  • Author(s): Murata-Kamiya, N.
  • Journal Title: Oncogene 26 Pages: 4617-4626
  • Peer Reviewed
• [Journal Article] Helicobacter pylori CagAはPAR1b/MARK2キナーゼを標的として上皮細胞の極性を破壊する。 (2007)
  • Author(s): 紙谷 尚子
  • Journal Title: 分子消化器病学 4 Pages: 114-118
• [Presentation] Elucidation of Helicobacter pylori CagA structure involved in deregulation of the ss-catenin signal. (2008)
  • Author(s): 紙谷尚子
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] Destabilization of the E-cadherin/ss-catenin complex by the Helicobacter pylori CagA protein. (2008)
  • Author(s): 紙谷尚子
  • Organizer: Cell polarity 2007 -International Symposium on Cell Polarity and Future Medicine-
  • Place of Presentation: 湘南国際村
• [Presentation] Elucidation of Helicobacter pylori CagA structure involved in deregulation of the β-catenin signal (2008)
  • Author(s): Kamiya, N
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] The role of PAR1/MARK polarity kinase in the interaction between Helicobacter pylori CagA and SHP-2 oncoprotein. (2007)
  • Author(s): 紙谷尚子
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Destabilization of the E-cadherin/b-catenin complex by the Helicobacter pylori CagA protein. (2007)
  • Author(s): Kamiya, N.
  • Organizer: Cell Polarity 2007 -International Symposium on Cell Polarity System and Future Medicine-
  • Place of Presentation: 湘南国際村・Shonan Village Center
• [Presentation] The role of PAR1/MARK polarity kinase in the interaction between Helicobacter pylori CagA and SHP2 oncoprotein (2007)
  • Author(s): Kamiya, N.
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜