Analysis on the induction mechanism of innate immune response to Listeria monocytogenes through Toll-like receptors

• Project/Area Number: 19790319
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: Kyoto University
• Principal Investigator: TSUCHIYA Kohsuke Kyoto University, 大学院・医学研究科, 助教 (50437216)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,780,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: Toll-like receptors / リステリア / 細胞内寄生菌 / サイトカイン / トル様受容体 / 自然免疫 / 細胞内寄生細菌 / ガンマー・インターフェロン / MyD88 / 多重欠損マウス / Toll-like receptor / ガンマーインターフェロン / 腫瘍壊死因子 / ノックアウトマウス

Research Abstract

Toll-like receptors(TLR)は、病原体由来の構造パターン(PAMPs)を認識し、侵入した病原体に対する防御に働くと考えられているが、病原体は通常複数のPAMPsを有しており、細胞内で特殊な動態を示す場合もある。そこで、細胞内寄生細菌であるリステリアの感染モデルを用いて各TLRの役割を解析したところ、複数のTLRを含むMyD88依存的なレセプターが補償的に働くことで宿主防御が強固に維持されていることが明らかになった。

Research Products

• [Journal Article] Dependency of caspase-1 activation induced in macrophages by Listeria monocytogenes on cytolysin, listeriolysin O, after evasion from phagosome into the cytolpas, (2008)
  • Author(s): Hara H, Tsuchiya K, Nomura T, Kawamura I, Shoma S, Mitsuyama M.
  • Journal Title: The Journal of Immunology 180 Pages: 7859-7868
  • Peer Reviewed
• [Journal Article] Critical involvement of pneumolysin in production of interleukin-lalpa and caspase-1-depentdent cytokines in infection with Streptococcus pneumoniae in vitro : a novel function of pneumolysin in caspase-1 activation (2008)
  • Author(s): Shoma S, Tsuchiya K, Kawamura I, Nomura T, Hara H, Uchiyama R, Daim S, Mitsuyama M.
  • Journal Title: Infection and Immunity 76 Pages: 1547-1557
  • Peer Reviewed
• [Journal Article] Dependency of caspase-1 activation induced in macrophages by Listeria monocytogenes on cytolysin, listeriolysin O, after evasion from phagosome into the cytoplasm (2008)
  • Author(s): Hara H, Tsuchiya K, Nomura T, Kawamura I, Shoma S, Mitsuyama M
  • Journal Title: The Journal of Immunology 180 Pages: 7859-7868
  • Peer Reviewed
• [Journal Article] Critical involvement of pneumolysin in production of interleukin-1alpha and caspase-1-dependent cytokines in infection with Streptococcus pneumoniae in vitro : a novel function of pneumolysin in casoase-1 activation (2008)
  • Author(s): Shoma S, Tsuchiya K, Kawamura I, Nomura T, Hara H, Uchiyama R, Daim S, Mitsuyama M
  • Journal Title: Infection and Immunity 76 Pages: 1547-1557
  • Peer Reviewed
• [Journal Article] Critical Involvement of Pneumolysin in Production of Interleukin-1α and Caspase-I-Dependent Cylokines in Infeclion with Streptococcus pneumoniae in vitro: a Novel function of pneumolysin in Caspase-1 Activation (2008)
  • Author(s): Shereen Shoma. Kohsuke Tsuchiya. Ikuo Kawamura, Takamasa Nomura, Hideki Hara, Pyosuke Uchiyama. Sylvia Daim, and Masao Mitsuyama
  • Journal Title: Infection and Immunity 76 Pages: 1547-1557
  • Peer Reviewed
• [Journal Article] Cytolysin-dependent escape of the bacterium from the phagosome is required but not sufficient for induction of the Th1 Immune rewponse against Listeria monocytogenes infection : distinct role of Listeriolysin O determined by cytolysin gene replacement (2007)
  • Author(s): Hara H, Kawamura I, Nomura T, Tominaga T, Tsuchiya K, Mitsuyama M.
  • Journal Title: Infection and Immunity 75 Pages: 3791-3801
  • Peer Reviewed
• [Journal Article] Involvement of caspase-9 in the inhibition of necrosis of RAW 264 cells infected with Mycobacterium tuverculosis (2007)
  • Author(s): Uchiyama R, Kawamura I, Fujimura T, Kawanishi M, Tsuchiya K, Tominaga T, Kaku T, Fukasawa Y, Sakai S, Nomura T, Mitsuyama M.
  • Journal Title: Infection and Immunity 75 Pages: 2894-2902
  • Peer Reviewed
• [Journal Article] Cytolysin-Depcndent Escape of the Bacterium From the Phagosome Is Required but Not Surricient For Induction of the thi Immune Response against Listeria monocytogenes Infection: Distinct Role of Listeriolysin O Determined by Cytolysin Gene Replacement (2007)
  • Author(s): Hideki Hara. Ikuo Kawamura, Takamasa Nomura, Takanari Tominaga, Kohsuke Tsuchiya, and Masao Mitsuyama
  • Journal Title: Infection and Immunity 75 Pages: 3791-3801
  • Peer Reviewed
• [Journal Article] Involvement of Caspase-9 in the Inhibition of Necrosis of RAW 264 Cells Infected with Mycobacterium tubereulosis (2007)
  • Author(s): Ryosuke Uchiyama, Ikuo Kawamura, Takao Fujimura, Michiko Kawanishi. Kohsuke Tsuchiya, Takanari Tominaga, Taijin Kaku, Yutaka Fukasawa, Shunsuke Sakai, Takamasa Nomura. and Masao Mitsuyama
  • Journal Title: Infection and Immunity 75 Pages: 2894-2902
  • Peer Reviewed
• [Presentation] MyD88 signals are essential but redundant in host defense againstListeria monocytogenes (2009)
  • Author(s): Tsuchiya K., Nomura T., Hara H., Shen Y., Sakai S., Kurenuma T., Yamamoto T., Kawamura I., Mitsuyama M.
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2009-03-12
• [Presentation] MyD88 signals are essential but redundant in host defense against Listeria monocytogenes (2009)
  • Author(s): Isuchiya K., Nomura T., Hara H., Shen Y., Sakai S., Kdrenuma T., Yamamoto T., Kawamura I., and Mitsuyama M
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2009-03-12
• [Presentation] Critical involvement of pneumolysin in the production of caspase-1-dependent cytokines in infection with Streptococcus pneumoniae. (2008)
  • Author(s): Tsuchiya K., Shoma S., Kawamura I., Nomura T., Mitsuyama M.
  • Organizer: The 8th Awaji Interanational Forum on Infection and Immunity
  • Place of Presentation: 兵庫県淡路市
  • Year and Date: 2008-09-07
• [Presentation] Critical involvement of pneurnolysin in the production of caspase-1-dependent cytokines in infection with Streptococcus pneumoniae (2008)
  • Author(s): Tsuchiya K., Shoma S., Kawamura I., Nomura T., Mitsuyama M
  • Organizer: The 8th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 兵庫県淡路島
  • Year and Date: 2008-09-07
• [Presentation] Critical involvement of pneumolysin in the production of caspase-1-dependent cytokines in infection with Streptococcus pneumoniae (2008)
  • Author(s): Tsuchiya K., Kawamura I., Nomura T., Mitsuyama M.
  • Organizer: XII. International Congress of Bacteriology and Applied Microbiology
  • Place of Presentation: イスタンブール・トルコ
  • Year and Date: 2008-08-05
• [Presentation] Escape of Listeria monocytogenes producing broad-range phospholipase C from autophagy-dependent killing (2007)
  • Author(s): Tsuchiya K., Kawamura I., Nomura T., Hara H., Mitsuyama M.
  • Organizer: 日本免疫学会総会・学術集会
  • Place of Presentation: 東京・品川
  • Year and Date: 2007-11-21
• [Presentation] Escape of Listeria monocytogenes producing broad-range phospholipase C from autophagy dependent killing (2007)
  • Author(s): Kohsuke Tsuchiya, Ikuo Kawamura, Takamasa Nomura, Ilideki Ilara, Masao. Nitsuyama
  • Organizer: 日本免疫学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-21