Analysis of TTSS-dependent suppression of inflammatory cytokine production by enterohemorrhagic Escherichia coli.

• Project/Area Number: 19790320
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: Osaka University
• Principal Investigator: KODAMA Toshio Osaka University, 微生物病研究所, 助教 (20346133)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,570,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥2,400,000 (Direct Cost: ¥2,400,000)
• Keywords: 腸管出血性大腸菌 / 3型分泌装置 / エフェクター / 炎症性サイトカイン

Research Abstract

腸管出血性大腸菌感染症の臨床症状では、炎症反応が低いという特徴を有する。しかしながら、その機構は全く明らかにされいない。これまでの解析結果により、この炎症抑制作用は3 型分泌装置(Type III secretion system; TTSS)依存的であり、さらに未知のエフェクターが寄与している可能性が示唆されている。本研究では、3型分泌装置依存的サイトカイン産生抑制機構に寄与するエフェクターの同定を試みた。

Research Products

• [Journal Article] Secreted indole serves as a signal for expression of type III secretion system translocators in enterohaemorrhagic Escherichia coli O157:H7. (2009)
  • Author(s): Hirakawa H, Kodama T, Takumi-Kobayashi A, Honda T, Yamaguchi A.
  • Journal Title: Microbiology 155 Pages: 541-550
  • Peer Reviewed
• [Journal Article] Sixty Years of vibrio parahaemolyticus Research. (2009)
  • Author(s): Honda T, Iida T, Akeda Y, Kodama T.
  • Journal Title: Microbe 3 Pages: 462-466
• [Journal Article] Identification and characterization of a novel type III secretion system in trh-positive Vibrio parahaemolyticus strain TH3996 reveal genetic lineage and diversity of pathogenic machinery beyond the species level. (2009)
  • Author(s): Okada N, Iida T, Park KS, Goto N, Yasunaga T, Hiyoshi H, Matsuda S, Kodama T, Honda T.
  • Journal Title: Infect Immun. 77 Pages: 904-913
  • Peer Reviewed
• [Journal Article] Secreted indole serves as a signal for expression of type III secretion system translocators in enterohaemorrhagic Escherichia coli O157:H7 (2009)
  • Author(s): Hirakawa H, Kodama T, Takumi-Kobayashi A, Honda T, Yamaguchi A.
  • Journal Title: Microbiology 155 Pages: 541-540
  • Peer Reviewed
• [Journal Article] Identification and characterization of a novel type III secretion system in trh-positive Vibrio parahaemolyticus strain TH3996 reveal genetic lineage and diversity of pathogenic machinery beyond,the species level (2009)
  • Author(s): Okada N, Iida T, Park KS, Goto N, Yasunaga T, Hiyoshi H, Matsuda S, Kodama T, Honda T.
  • Journal Title: Infect Immun 77 Pages: 904-913
  • Peer Reviewed
• [Journal Article] Identification of two translocon proteins of Vibrio parahaemolyticus type III secretion system 2. (2008)
  • Author(s): Kodama T, Hiyoshi H, Gotoh K, Akeda Y, Matsuda S, Park KS, Cantarelli VV, Iida T, Honda T.
  • Journal Title: Infect Immun. 76 Pages: 4282-4289
  • Peer Reviewed
• [Journal Article] Sixty Years of vibrio parahaemolyticus Research (2008)
  • Author(s): Honda T, Eda T, Akeda Y, Kodama T
  • Journal Title: Microbe 3 Pages: 462-466
• [Journal Article] Identification of two translocon proteins of Vibrio parahaemolyticus type III secretion system 2 (2008)
  • Author(s): Kodama T, Hiyoshi H, Gotoh K, Akeda Y, Matsuda S, Park KS, Cantarelli VV, Iida T, Honda T.
  • Journal Title: Infect Immun 76 Pages: 4282-4289
  • Peer Reviewed
• [Journal Article] Identification and characterization of VopT, a novel ADP-ribosyltransferase effector protein secreted via the Vibrio parahaemolyticus type III secretion system 2. (2007)
  • Author(s): Kodama. T., Rokuda. M., Park. K. S., Cantarelli. V. V., Mastuda. S, Iida. T. and Honda. T.
  • Journal Title: Cell. Microbiol. 9(11) Pages: 2598-2609
  • Peer Reviewed
• [Journal Article] Tyrosine phosphorylation controls cortactin binding to two enterohaemorrhagic Escherichia coli effectors : Tir and EspFu/TccP. (2007)
  • Author(s): Cantarelli. V. V., Kodama. T., Nijstad. N., Abolghait. S. K., Noda. S., Okada. M., Iida. T. and Honda. T.
  • Journal Title: Cell. Microbiol. 9(7) Pages: 1782-1795
  • Peer Reviewed
• [Journal Article] Demonstration and characterization of manganese superoxide dismutase of Providencia alcalifaciens. Microbiol. (2007)
  • Author(s): Chen. X., Kodama. T., Iida. T. and Honda. T.
  • Journal Title: Immunol. 51(10) Pages: 951-961
  • Peer Reviewed
• [Journal Article] A targeting approach for delivery of polymer microparticle- antibody conjugate against Vibrio parahaemolyticus-induced cytotoxicity to human intestinal epithelial cells. (2007)
  • Author(s): Gao. F., Kodama. T., Chen. X., Okada. K. and Honda. T.
  • Journal Title: J. Drug. Target. 15(6) Pages: 428-436
  • Peer Reviewed
• [Journal Article] Identification and characterization of VopT, a novel ADP-ribosyltransferase effector protein secreted via the Vibrio parahaemolyticus type III secretion system 2. (2007)
  • Author(s): Kodama. T., Rokuda. M., Park. K. S., Cantarelli. V. V., Mastuda. S, Iida. T., Honda. T.
  • Journal Title: Cell. Microbiol. 9(11) Pages: 2598-2609
  • Peer Reviewed
• [Journal Article] Tyrosine phosphorylation controls cortactin binding to two enterohaemorrhagic Escherichia coli effectors:Tir and EspFu/TccP (2007)
  • Author(s): Cantarelli. V. V., Kodama. T., Nijstad. N., Abolghait. S. K., Noda. S., Okada. M., Iida. T., Honda. T.
  • Journal Title: Cell. Microbiol. 9(7) Pages: 1782-1795
  • Peer Reviewed
• [Journal Article] Demonstration and characterization of manganese superoxide dismutase of Providencia alcalifaciens. (2007)
  • Author(s): Chen. X., Kodama. T., Iida. T., Honda. T.
  • Journal Title: Microbiol. Immunol. 51(10) Pages: 951-961
  • NAID: 10020278623
  • Peer Reviewed
• [Journal Article] A targeting approach for delivery of polymer microparticle- antibody conjugate against Vibrio parahaemolyticus-induced cytotoxicity human intestinal epithelial cells. (2007)
  • Author(s): Gao. F., Kodama. T., Chen. X., Okada. K., Honda. T.
  • Journal Title: J. Drug. Target. 15(6) Pages: 428-436
  • Peer Reviewed
• [Presentation] 腸炎ビブリオのTTSS2遺伝子群の発現促進因子の検索 (2009)
  • Author(s): 後藤和義、児玉年央、日吉大貴、井筒香織、本田武司、飯田哲也
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] 腸炎ビブリオのTTSS2遺伝子群の発現制御機構の解析 (2009)
  • Author(s): 児玉年央、後藤和義、日吉大貴、井筒香織、本田武司、飯田哲也
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] TRH産生腸炎ビブリオのPathogenicity Islandの解析 (2009)
  • Author(s): 岡田奈津実、飯田哲也、児玉年央、本田武司
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] 腸炎ビブリオT3SSエフェクター分泌に必須のシャペロンの同定 (2009)
  • Author(s): 木村友美、明田幸宏、児玉年央、飯田哲也、本田武司
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] 腸炎ビブリオ耐熱性溶血毒の細胞膜上の局在の解析 (2009)
  • Author(s): 松田重輝、飯田哲也、児玉年央、本田武司
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] 腸炎ビブリオT3SS2シャペロンの同定とその機能解析 (2009)
  • Author(s): 明田幸宏、児玉年央、大石和徳、飯田哲也、本田武司
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] Contribution of Vibrio parahaemolyticus virulence factors to cytotoxicity and enterotoxicity. (2009)
  • Author(s): Toshio Kodama, Hirotaka Hiyoshi, Tetsuya Iida, and Takeshi Honda.
  • Organizer: 2009 International CVRDC-RIMD joint symposium
  • Place of Presentation: Korea
• [Presentation] Contribution of Vibrio parahaemolyticus virulence factors to cytotoxicity and enterotoxicity (2009)
  • Author(s): Toshio Kodama, Hirotaka Hiyoshi, Tetsuya Iida, and Takeshi Honda
  • Organizer: 2009 International CVRDC-RIMD joint symposium
  • Place of Presentation: 韓国・慶州
• [Presentation] TRH産生腸炎ビブリオのBathogenicity Islandの解析 (2009)
  • Author(s): 岡田奈津実、飯田哲也、児玉年央、本田武司
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] TRH産生腸炎ビブリオのPathogenicity Islandの解析 (2008)
  • Author(s): 岡田奈津実、飯田哲也、児玉年央、本田武司
  • Organizer: 日本細菌学会関西支部
  • Place of Presentation: 京都
  • Year and Date: 2008-11-08
• [Presentation] 腸炎ビブリオのTTSS2遺伝子群の発現促進因子の検索 (2008)
  • Author(s): 後藤和義、児玉年央、日吉大貴、井筒香織、本田武司、飯田哲也
  • Organizer: 日本細菌学会関西支部
  • Place of Presentation: 京都
  • Year and Date: 2008-11-08
• [Presentation] 腸炎ビブリオT3SSエフェクター分泌に必須のシャペロンの同定 (2008)
  • Author(s): 木村友美、明田幸宏、児玉年央、飯田哲也、本田武司
  • Organizer: 日本細菌学会関西支部
  • Place of Presentation: 京都
  • Year and Date: 2008-11-08
• [Presentation] TRH産生腸炎ビブリオのBathogenicity Islandの解析 (2008)
  • Author(s): 岡田奈津実、飯田哲也、児玉年央、本田武司
  • Organizer: 日本細菌学会関西支部
  • Place of Presentation: 京都
  • Year and Date: 2008-11-08
• [Presentation] TRH産生腸炎ビブリオのPathogenicity Islandの解析 (2008)
  • Author(s): 岡田奈津実、飯田哲也、児玉年央、本田武司
  • Organizer: 腸炎ビブリオシンポジウム
  • Place of Presentation: 富山
  • Year and Date: 2008-10-23
• [Presentation] 腸炎ビブリオのTTSS2遺伝子群の発現促進因子の検索 (2008)
  • Author(s): 後藤和義、児玉年央、日吉大貴、井筒香織、本田武司、飯田哲也
  • Organizer: 腸炎ビブリオシンポジウム
  • Place of Presentation: 富山
  • Year and Date: 2008-10-23
• [Presentation] 腸炎ビブリオT3SSエフェクター分泌に必須のシャペロンの同定 (2008)
  • Author(s): 木村友美、明田幸宏、児玉年央、飯田哲也、本田武司
  • Organizer: 腸炎ビブリオシンポジウム
  • Place of Presentation: 富山
  • Year and Date: 2008-10-23
• [Presentation] 腸炎ビブリオ耐熱性溶血毒の細胞毒性発現には細胞膜ラフトとのassociationが必要である (2008)
  • Author(s): 松田重輝、飯田哲也、児玉年央、本田武司
  • Organizer: 腸炎ビブリオシンポジウム
  • Place of Presentation: 富山
  • Year and Date: 2008-10-23
• [Presentation] TRH産生腸炎ビブリオのBathogenicity Islandの解析 (2008)
  • Author(s): 岡田奈津実、飯田哲也、児玉年央、本田武司
  • Organizer: 腸炎ビブリオシンポジウム
  • Place of Presentation: 富山
  • Year and Date: 2008-10-23
• [Presentation] 腸炎ビブリオ耐熱性溶血毒の細胞毒性発現には細胞膜ラフトとのassociationが必要である (2008)
  • Author(s): 松田重輝、飯田哲也、児玉年央、本田武司
  • Organizer: 陽炎ビブリオシンポジウム
  • Place of Presentation: 富山
  • Year and Date: 2008-10-23
• [Presentation] Contribution of Vibrio parahaemolyticus virulence factors to cytotoxicity and enterotoxicity. (2008)
  • Author(s): Toshio Kodama, Hirotaka Hiyoshi, Tetsuya Iida, and Takeshi Honda.
  • Organizer: US-Japan cooperative medical science program -cholera and other bacteria enteric infections
  • Place of Presentation: 博多
• [Presentation] 腸炎ビブリオの病原性(下痢原性)に寄与する因子の同定 (2008)
  • Author(s): 児玉年央、日吉大貴、飯田哲也、本田武司
  • Organizer: 日本感染症学会中日本地方会学術集会
  • Place of Presentation: 大阪
• [Presentation] Contribution of Vibrio parahaemolyticus virulence factors to cytotoxicity and enterotoxicity. (2008)
  • Author(s): Hirotaka Hiyoshi, Toshio Kodama, Tetsuya Iida, and Takeshi Honda.
  • Organizer: Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji
• [Presentation] Identification of two translocon proteins of Vibrio parahaemolyticus Type III secretion system 2. (2008)
  • Author(s): Toshio Kodama, Hirotaka Hiyoshi, Tetsuya Iida, and Takeshi Honda.
  • Organizer: Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji
• [Presentation] Identification and characterization of a Type III secretion-associated chaperone in the Type III secretion system 1 of Vibrio parahaemolyticus. (2008)
  • Author(s): Yukihiro Akeda, Kannna Okayama, Tomomi Kimura, Rikard Dryselius, Toshio Kodama, Kazunori Oishi, Tetsuya Iida, and Takeshi Honda.
  • Organizer: Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji
• [Presentation] 腸炎ビブリオ耐熱性溶血毒の細胞毒性発現には細胞膜ラフトとのassociationが必要である (2008)
  • Author(s): 松田重輝、飯田哲也、児玉年央、本田武司
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 京都
• [Presentation] 腸炎ビブリオの耐熱性溶血毒と2つのIII型分泌装置の細胞毒性と腸管毒性における役割 (2008)
  • Author(s): 日吉大貴、児玉年央、飯田哲也、本田武司
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 京都
• [Presentation] Contribution of Vibrio parahaemolyticus virulence factors to cytotoxicity and enterotoxicity (2008)
  • Author(s): Toshio Kodama, Hirotaka Hiyoshi, Tetsuya Iida, and Takeshi Honda
  • Organizer: US-Japan cooperative medical science program -cholera and other bacterial enteric infections
  • Place of Presentation: 博多
• [Presentation] Contribution Of Vibrio parahaemolyticus virulence factors to cytotoxicity and enterotoxicity (2008)
  • Author(s): Hirotaka Hiyoshi, Toshio Kodama, Tetsuya Iida, and Takeshi Honda
  • Organizer: Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji
• [Presentation] Identification of two translocon proteins of Vibrio parahaemolyticus Type III secretion system 2 (2008)
  • Author(s): Toshio Kodama, Hirotaka Hiyoshi, Tetsuya Iida, and Takeshi Honda
  • Organizer: Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji
• [Presentation] Identification and characterization of a Type III secretion-associated chaperone in the Type III secretion system 1 of Vibrio parahaemolyticus (2008)
  • Author(s): Yukihiro Akeda, Kannna Okayama, Tomomi Kimura, Rikard Dryselius, Toshio Kodama, Kazunori Oishi, Tetsuya Iida, and Takeshi Honda
  • Organizer: Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji
• [Presentation] 腸炎ビブリオの耐熱性溶血毒と2つのIII型分泌装置の細胞毒性と腸管毒性活性における役割 (2007)
  • Author(s): 日吉大貴、児玉年央、飯田哲也、本田武司
  • Organizer: 日本細菌学会関西支部
  • Place of Presentation: 大阪
  • Year and Date: 2007-11-10
• [Presentation] Identification of two translocon proteins of Vibrio parahaemolyticus Type III secretion system 2. (2007)
  • Author(s): Kodama. T., Hiyoshi. H., Matsuda. S., Akeda. Y., Iida. T. and Honda. T.
  • Organizer: US-Japan cooperative medical science program -cholera and other bacterial enteric infections
  • Place of Presentation: Austin, Texas, USA.
• [Presentation] Identification of two translocon proteins of Vibrio parahaemolyticus Type III secretion system 2. (2007)
  • Author(s): Kodama. T., Hiyoshi. H., Matsuda. S., Akeda. Y., Iida. T. and Honda. T.
  • Organizer: Vibrio 2007 -the second conference on the biology of Vibrios
  • Place of Presentation: Paris, France.
• [Presentation] 腸炎ビブリオの耐熱性溶血毒と2つのIII型分泌装置の細胞毒性と腸管毒性活性における役割 (2007)
  • Author(s): 日吉大貴、児玉年央、飯田哲也、本田武司
  • Organizer: 腸炎ビブリオシンポジウム
  • Place of Presentation: 神戸
• [Presentation] 腸管出血性大腸菌が産生するエフェクターNleAの宿主細胞内修飾 (2007)
  • Author(s): 児玉年央、阪本怜、飯田哲也、本田武司
  • Organizer: 腸管出血性大腸菌シンポジウム
  • Place of Presentation: 長野
• [Presentation] 腸炎ビブリオTTSS2遺伝子群の発現制御機構の解析 (2007)
  • Author(s): 児玉年央、森田幹晴、井筒香織、朴権三、飯田哲也、本田武司
  • Organizer: 日本細菌学会
  • Place of Presentation: 大阪
• [Presentation] 腸管出血性大腸菌が産生するエフェクターNleAの宿主細胞内修飾 (2007)
  • Author(s): 阪本怜、児玉年央、飯田哲也、本田武司
  • Organizer: 日本細菌学会
  • Place of Presentation: 大阪
• [Presentation] Providencia alcalifaciensの3型分泌装置依存的細胞毒性機構の解析 (2007)
  • Author(s): 陳修浩、児玉年央、飯田哲也、本田武司
  • Organizer: 日本細菌学会
  • Place of Presentation: 大阪
• [Presentation] Identification of two translocon proteins of Vibrio parahaemolyticus Type III secretion system 2 (2007)
  • Author(s): Kodama. T., Hiyoshi. H., Matsuda. S., Akeda. Y., Iida. T., Honda. T.
  • Organizer: Vibrio 2007 -the second conference on the biology of Vibrios
  • Place of Presentation: Paris, France
• [Presentation] Identification of two translocon proteins of Vibrio parahaemolyticus Type III secretion system 2 (2007)
  • Author(s): Kodama. T., Hiyoshi. H., Matsuda. S., Akeda. Y., Iida. T., Honda. T.
  • Organizer: US-Japan cooperative medical science program -cholera and other bacterial enteric infections
  • Place of Presentation: Austrin, Texas, USA