Genomic comparison of the O-antigen biosynthesis gene clusters of Escherichia coli strains

• Project/Area Number: 19790325
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: University of Miyazaki
• Principal Investigator: IGUCHI Atsushi University of Miyazaki, フロンティア科学実験総合センター, 研究員 (00437948)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,630,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: 遺伝 / 大腸菌 / O抗原 / 水平伝播 / 比較ゲノム

Research Abstract

本研究では、O抗原コード領域とその周辺領域を株間で比較することにより、水平伝播領域の特定および入れ換わった領域の特徴を調べた。その結果、複数の大腸菌株でO抗原コード領域を含む大きな領域が水平伝播により入れ換わったことが明らかとなった。

Research Products

• [Journal Article] The complete genome sequence and com parative genome analysis of enteropathoge nic E (2009)
  • Author(s): Iguchi A, Thomson N R, Ogura Y, Saun ders D, Ooka T, Henderson I R, Harris D, Asadulghani M, Kurokawa K, Dean P Kenny B, Quail M A, Thurston S, Dou gan G, Hayashi T, Parkhill J, Frankel G
  • Journal Title: coli O127:H6 strain E2348/69 vol.191 Pages: 347-354
  • Peer Reviewed
• [Journal Article] The Emergence and Constrained Radiations of Escherichia coli O157 : A Precise Reconstruction (2009)
  • Author(s): Leopold R S, Magrini V, Holt J N, Shaikh N, Mardis R E, Cagno J, Ogura Y, Iguchi A, Hayashi T, Mellmann A, Karch H, Besser E T, Sawyer A S, Whittam S T, Tarr I P
  • Journal Title: PNAS vol.106 Pages: 8713-8718
  • Peer Reviewed
• [Journal Article] The complete genome sequence and comparative genome analysis of enteropathogenic E. coli 0127 : H6 strain E2348/69 (2009)
  • Author(s): Iguchi A., et.al.
  • Journal Title: J. Bacteriol 191(1) Pages: 347-354
  • Peer Reviewed
• [Journal Article] Genomic comparison of the O-antigen biosynthesis gene clusters of Escherichia coli O55 strains belonging to three distinct lineages (2008)
  • Author(s): Iguchi A, Ooka T, Ogura Y, Asadulghani, Nakayama K, Frankel G, Hayashi T
  • Journal Title: Microbiology vol.154 Pages: 559-570
  • Peer Reviewed
• [Journal Article] Genomic comparison of the O-antigen biosynthesis gene clusters of Escherichia coli 055 strains belonging to three distinct lineages (2008)
  • Author(s): Iguchi A., et.al.
  • Journal Title: Microbiology 154(2) Pages: 559-570
  • Peer Reviewed
• [Journal Article] Systematic identification and sequence analysis of the genomic islands of the enteropathogenic Escherichia coli strain B171-8 by the combined use of Whole Genome PCR Scanning and fosmid mapping (2008)
  • Author(s): Ogura Y, et.al.
  • Journal Title: J. Bacteriol 190(21) Pages: 6948-6960
  • Peer Reviewed
• [Journal Article] Genomic comparison of the O-antigen biosynthesis gene clusters of Escherichia coli 055 strains belonging to three distinct lineages (2008)
  • Author(s): Atsushi Iguchi
  • Journal Title: Microbiology 154 Pages: 559-570
  • Peer Reviewed
• [Journal Article] The defective prophage pool of Escherichia coli O157 : prophage-prophage interactions potentiate horizontal transfer of virulence determinants
  • Author(s): Asadulghani M, Ogura Y, Ooka T, Itoh T, Sawaguchi A, Iguchi A, Nakayama K,Hayashi T
  • Journal Title: PLoS Pathogen (in press)
  • Peer Reviewed
• [Journal Article] The defective prophage pool of Escherichia coli 0157 : prophage-p rophage interactions potentiate horizontal transfer of virulence deter minants
  • Author(s): Asadulghani, et.al.
  • Journal Title: PLoS Pathog. (in press)
  • Peer Reviewed
• [Presentation] 腸管病原性大腸菌(EPEC)E2348/69株のゲノム解析 (2009)
  • Author(s): 井口純,他
  • Organizer: 第3回ゲノム微生物学会年会
• [Presentation] The complete genome sequence and comparative genome analysis of EPEC O127 : H6 strain E2348/69 (2009)
  • Author(s): 井口純,他(ワークショップ)
  • Organizer: 第82回日本細菌学会総会
• [Presentation] 腸管病原性大腸菌(EPEC)E2348/69株のゲノム解析 (2009)
  • Author(s): 井口純, 他
  • Organizer: 第3回ゲノム微生物学会年会
  • Place of Presentation: 東京
• [Presentation] The complete genome sequence and comparative genome analysis of EPEC O127 : H6 strain E2348/69 (2009)
  • Author(s): 井口純, 他(ワークショップ)
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] 大腸菌におけるO抗原をコードする領域の組換え過程の解析 等 (2008)
  • Author(s): 井口 純
  • Organizer: 日本ゲノム微生物学会 等
  • Place of Presentation: 大阪大学 等
  • Year and Date: 2008-03-06
• [Presentation] 大腸菌におけるO抗原をコードする領域の組換え過程の解析 (2008)
  • Author(s): 井口純,他
  • Organizer: 第2回ゲノム微生物学会年会
• [Presentation] 大腸菌におけるO抗原をコードする領域の組換え過程 (2008)
  • Author(s): 井口純,他
  • Organizer: 第81回日本細菌学会総会
• [Presentation] 大腸菌におけるO抗原合成遺伝子群を含む水平伝播領域の解析 (2007)
  • Author(s): 井口純,他
  • Organizer: 第11回腸管出血性大腸菌感染症シンポジウム