Rapid Detection of Point Mutations Conferring Resistance to Fluoroquinolone in the gyrA of Helicobacter pylori

• Project/Area Number: 19790327
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: Keio University
• Principal Investigator: NISHIZAWA Toshihiro Keio University, 医学部, 助教 (80348648)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,030,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2007: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: ヘリコバクター・ピロリ / フルオロキノン / gyrA遺伝子 / H.phlore / フルオロキノロン / H.pylori

Research Abstract

内視鏡後のH. pylori培養検体においてアレル特異的PCR法によりキノロン耐性にかかわるgyrA遺伝子変異株と野生株を2本のPCRチューブで3〜4時間で簡便に識別可能であった。糞便検体については他の腸内細菌のgyrA遺伝子も増幅されてしまい判定不能であった。従来のH. pylori薬剤感受性試験は費用がかかり、10〜14日間の日数を要するが、H. pyloriのキノロン耐性に関わるgyrA遺伝子変異においてアレル特異的PCR法による迅速検出法は簡便で有用と考えられた。

Research Products

• [Journal Article] Early Helicobacter pylori eradication restores sonic hedgehog expression in the gastric mucosa of Mongolian gerbils. (2009)
  • Author(s): Nishizawa T, Suzuki H, Nakagawa I, Minegishi Y, Masaoka T, Iwasaki E, Hibi T
  • Journal Title: Digestion 79(2) Pages: 99-108
  • Peer Reviewed
• [Journal Article] Fluoroquinolone-based third-line therapy for Helicobacter pylori eradication. (2009)
  • Author(s): Nishizawa T, Suzuki H, Hibi T
  • Journal Title: J Clin. Biochem. Nutr. 44(2) Pages: 119-124
  • Peer Reviewed
• [Journal Article] ワーファリン内服中のメトロニダゾールによる除菌治療の注意点 (2009)
  • Author(s): 西澤俊宏、鈴木秀和
  • Journal Title: Helicobacter Research. 13(1) Pages: 63-65
  • Peer Reviewed
• [Journal Article] Past Rifampicin Dosing Determines Rifabutin Resistance of Helicobacter pylori (2009)
  • Author(s): Suzuki S, Suzuki H, Nishizawa T, Kaneko F, Ootani S, Muraoka H, Saito Y, Kobayashi I, Hibi T
  • Journal Title: Digestion 14.79(1) Pages: 1-4
  • Peer Reviewed
• [Journal Article] Sitafloxacin and garenoxacin may overcome the antibiotic resistance of H. pylori with gyrA mutation (2009)
  • Author(s): Suzuki H, Nishizawa T, Muraoka H, Hibi T
  • Journal Title: Antimicrob Agents Chemother 53(4) Pages: 1720-1721
  • Peer Reviewed
• [Journal Article] Early Helicobacter pylori eradication restores sonic hedgehog expression in the gastric mucosa of Mongolian aerbils (2009)
  • Author(s): Toshihiro Nishizawa
  • Journal Title: Digestion 79(2) Pages: 99-108
  • Peer Reviewed
• [Journal Article] Rebamipide promoted restoration of sonic hedgehog expression in the gastric mucosa after early eradication of Helicobacter pylori infection (2009)
  • Author(s): Nishizawa T
  • Journal Title: Digestion (In press)
  • Peer Reviewed
• [Journal Article] Fluoroquinolone-based third-line therapy for Helicobacter pylori eradication (2009)
  • Author(s): Nishizawa T
  • Journal Title: J Clin. Biochem. Nutr. 44(2) Pages: 119-124
  • Peer Reviewed
• [Journal Article] Sitafloxacin and garenoxacin may overcome the antibiotic resistance of H. pylori with gyrA mutation (2009)
  • Author(s): Suzuki H, Nishizawa T
  • Journal Title: Antimicrob Agents Chemother 53(4) Pages: 1720-1721
  • Peer Reviewed
• [Journal Article] Past Rifampicin Dosing Determines Rifabutin Resistance of Helicobacter pylori (2009)
  • Author(s): Suzuki S, Suzuki H, Nishizawa T
  • Journal Title: Digestion 79(1) Pages: 1-4
  • Peer Reviewed
• [Journal Article] ワーファリン内服中のメトロニダゾールによる除菌治療の注意点 (2009)
  • Author(s): 西澤俊宏
  • Journal Title: Helicobacter Research 13(1) Pages: 63-65
• [Journal Article] Alpha-ketoglutarate oxidoreductase, an essential salvage enzyme of energy metabolism, in coccoid form of Helicobacter pylori (2008)
  • Author(s): Tsugawa H, Suzuki H, Nakagawa I, Nishizawa T , Saito Y, Suematsu M, Hibi T
  • Journal Title: Biochem Biophys Res Commun 376(1) Pages: 46-51
  • Peer Reviewed
• [Journal Article] Gatifloxacin-based triple therapy as third-line regimen for Helicobacter pylori eradication (2008)
  • Author(s): Nishizawa T, Suzuki H, Nakagawa I, Iwasaki E, Masaoka T, Hibi T
  • Journal Title: J Gastroenterol Hepatol 23:s2 Pages: 167-170
  • Peer Reviewed
• [Journal Article] IgG4-negative autoimmune pancreatitis with sclerosing cholangitis and colitis : possible association with primary sclerosing cholangitis? (2008)
  • Author(s): Saeki K, Hozawa S, Miyata N, Nishizawa, T, Soma H, Iwao Y, Kameyama K, Hibi T
  • Journal Title: Intern. Med. 47(10) Pages: 943-948
  • NAID: 130000078683
  • Peer Reviewed
• [Journal Article] FDの病態とグレリン (2008)
  • Author(s): 西澤俊宏、鈴木秀和
  • Journal Title: 消化器の臨床 Vol. No. 4
• [Journal Article] ガチフロキサシンはどういう薬剤ですか? H. pylori 除菌に有効ですか? (2008)
  • Author(s): 西澤俊宏、鈴木秀和
  • Journal Title: Helicobacter Research Vol. 12. no 2 Pages: 59-61
• [Journal Article] Helicobacter pylori研究の年間レビュー、非悪性胃十二指腸疾患とのかかわり (2008)
  • Author(s): 鈴木秀和、西澤俊宏
  • Journal Title: Helicobacter Research. Vol. 12. no 2 Pages: 26-28
• [Journal Article] Gatifloxacin-based triple therapy as third-line regimen for Helicobacter pylori eradication (2008)
  • Author(s): Nishizawa T
  • Journal Title: J Gastroenterol Hepatol 23(2) Pages: 167-170
  • Peer Reviewed
• [Journal Article] Alphaketoglutarate oxidoreductase, an essential salvage enzyme of energy metabolism, in coccoid form of Helicobacter pylori (2008)
  • Author(s): Tsugawa H, Suzuki H, Nakagawa I, Nishizawa T
  • Journal Title: Biochem Biophys Res Commun. 376(1) Pages: 46-51
  • Peer Reviewed
• [Journal Article] IgG4-negatie autoimmune pancreatitis with sclerosing cholangitis and colitis : possible association primary sclerosing cholangitis? (2008)
  • Author(s): Saeki K, Hozawa S, Miyata N, Nishizawa, T
  • Journal Title: Intern. Med. 47(10) Pages: 943-948
  • Peer Reviewed
• [Journal Article] FDの病態とグレリン (2008)
  • Author(s): 西澤俊宏
  • Journal Title: 消化器の臨床 4(11) Pages: 385-388
• [Journal Article] A new eradication resistance index as a predictor of metronidazole-containing second-line treatment of Helicobacter pylori. (2007)
  • Author(s): Nishizawa T, Suzuki H, Masaoka T, Iwasaki E, Hibi T
  • Journal Title: Digestion 76(3-4) Pages: 215-220
  • Peer Reviewed
• [Journal Article] Rapid detection of point mutations conferring resistance to fluoroquinolone in the gyrA of Helicobacter pylori by allele-specific polymerase Chain Reaction. (2007)
  • Author(s): Nishizawa T, Suzuki H, Ayako U, Muraoka M, Iwasaki E, Masaoka M, Kobayashi I, Hibi T.
  • Journal Title: J. Clin. Microbiol 45(2) Pages: 303-305
  • Peer Reviewed
• [Journal Article] Reduced conscious blood flow in the stomach during non-steroidal anti-inflammatory drugs administration assessed by flash echo imaging. (2007)
  • Author(s): Nishizawa T, Suzuki H, Masaoka T, Iwasaki E, Hibi T
  • Journal Title: Scand. J. Gasroenterol 42(9) Pages: 1040-1044
  • Peer Reviewed
• [Journal Article] Enhanced gastric transmural blood flow in aspirin intoxication (2007)
  • Author(s): Nishizawa T, Suzuki H, Masaoka T, Hibi T
  • Journal Title: J Gastroenterol Hepatol 22(11) Pages: 2037-2037
  • Peer Reviewed
• [Journal Article] Helicobacter pylori eradication restored Sonic hedgehog expression in the stomach. (2007)
  • Author(s): Nishizawa T, Suzuki H, Masaoka T, Miegishi Y, Eisuke I, Hibi T
  • Journal Title: Hepatogastroenterology 54(75) Pages: 697-700
  • Peer Reviewed
• [Journal Article] 膵癌との鑑別に苦慮した膵仮性嚢胞を伴うアルコール性膵炎の1例 (2007)
  • Author(s): 西澤俊宏,朴沢重成,鈴木秀和,今枝博之,樋口肇,久松理一,永田博司,島津元秀,向井万起男,日比紀文
  • Journal Title: 膵臓 22 Pages: 503-508
  • NAID: 10024366606
  • Peer Reviewed
• [Journal Article] Helicobacter pyloriの二次・三次除菌療法 (2007)
  • Author(s): 西澤俊宏、鈴木秀和
  • Journal Title: 消化管Network Vol. 8, No4
• [Journal Article] 肥満遺伝子産物、レプチン。その病態生理学的意義。 (2007)
  • Author(s): 鈴木秀和、西澤俊宏
  • Journal Title: 医学のあゆみ Vol. 223, No7 Pages: 521-524
  • Peer Reviewed
• [Journal Article] 臨床医学の展望、消化器病学、上部消化管疾患。 (2007)
  • Author(s): 鈴木秀和、正岡建洋、西澤俊宏、岩崎栄典、日比紀文
  • Journal Title: 日本医事新報 4322 Pages: 64-65
• [Journal Article] 抗へリコバクター食品 (2007)
  • Author(s): 鈴木秀和、西澤俊宏、日比紀文
  • Journal Title: G. I. Research 15(1) Pages: 55-59
• [Journal Article] Rapid detection of point mutations conferring resistance to f1 uoroquinolone in the gyrA of Helicobacter pylori by allele-specific poljrmerase Chain Reaction (2007)
  • Author(s): Toshihiro Nishizawa
  • Journal Title: J. Clin. Microbiol. 45(2) Pages: 303-305
  • Peer Reviewed
• [Journal Article] A new eradication resistance index as a predictor of metronidazole-containing second-line treatment of Helicobacter pylori (2007)
  • Author(s): Toshihiro Nishizawa
  • Journal Title: Digestion 76(3-4) Pages: 215-220
  • Peer Reviewed
• [Journal Article] Helicobacter pylori eradication restored Sonic hedgehog expression in the stomach (2007)
  • Author(s): Toshihiro Nishizawa
  • Journal Title: Hepatogastroenterology 54(75) Pages: 697-700
  • Peer Reviewed
• [Journal Article] Reduced conscious blood flow in the stomach during NSAIDsadministration assessed by flash echo imaging (2007)
  • Author(s): Toshihiro Nishizawa
  • Journal Title: Scand. J. Gasroenterol 42(9) Pages: 1040-1044
  • Peer Reviewed
• [Journal Article] Enhanced gastric transmural blood flow in aspirinIntoxication (2007)
  • Author(s): Toshihiro Nishizawa
  • Journal Title: J Gastroenterol Hepatol 22(11) Pages: 2037-2037
  • Peer Reviewed
• [Journal Article] Helicobacter plyori の二次・三次除菌療法 (2007)
  • Author(s): 西滓 俊宏
  • Journal Title: 消化管Network. Vol.8,No4 Pages: 79-80
• [Journal Article] レプチン。その病態生理学的意義。 (2007)
  • Author(s): 鈴木 秀和, 西澤 俊宏
  • Journal Title: 医学のあゆみ Vol.223,N07 Pages: 521-524
• [Journal Article] Rebamipide promoted restoration of sonic hedgehog expression in the gastric mucosa after early eradication of Helicobacter pylori infection
  • Author(s): Nishizawa T, Suzuki H, Nakagawa I, Minegishi Y, Masaoka T, Iwasaki E, Hibi T
  • Journal Title: Digestion (in prss)
  • Peer Reviewed
• [Presentation] Earlier H pylori eradication restores sonic hedgehog expression in the gastric mucosa (2008)
  • Author(s): Toshihiro Nishizawa
  • Organizer: Digestive Disease Week 2008
  • Place of Presentation: San Diego
  • Year and Date: 2008-05-19
• [Presentation] Earlier H. pylori eradication restores sonic hedgehog expression in the gastric mucosa (2008)
  • Author(s): Nishizawa, T. Hidekazu Suzuki, Yuriko Minegishi, Tatsuhiro Masaoka, Eisuke Iwasaki, Toshifumi Hibi
  • Organizer: Earlier H. pylori eradication restores sonic hedgehog expression in the gastric mucosa
  • Place of Presentation: Sandiego
  • Year and Date: 2008-05-18
• [Presentation] Prediction of the results of metronidazole-based second-line and gatifloxacin-based third-line therapies after the failure of H. pylori eradication. (2007)
  • Author(s): Nishizawa, T., Suzuki, H., Nakagawa I., Iwasaki, E., Masaoka, T., Hibi, T.
  • Organizer: The 1st Japan and US Collaboration Conference in Gastroenterology.
  • Place of Presentation: Tokyo
  • Year and Date: 2007-11-16
• [Presentation] Prediction of the results of MNZ-based second-line and GFLX-based third-line therapies after H.pylori eradication failure. (2007)
  • Author(s): Toshihiro Nishizawa
  • Organizer: Japan and US Collaboration Conference
  • Place of Presentation: Tokyo
  • Year and Date: 2007-11-16
• [Presentation] 感染スナネズミ胃炎でのsonic hedgehog及びRUNX3発現と除菌後の胃粘膜再生-胃がん予防の除菌時期の考察 (2007)
  • Author(s): 西澤俊宏, 鈴木秀和,日比紀文
  • Organizer: 49回日本消化器病学会大会シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2007-10-20
• [Presentation] 感染不ナネズミ胃炎でのsonic hedgehog及びRUNX3発現と除菌後の胃粘膜再生-胃がん予防の除菌時期の考察 (2007)
  • Author(s): 西澤 俊宏
  • Organizer: 49回日本消化器病学会大会, シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2007-10-20
• [Presentation] Sonic hedgehog expression in the gastric mucosa with or without Heterotopic proliferative glands after the Helicobacter pylori eradication. (2007)
  • Author(s): Nishizawa, T., Hidekazu Suzuki, Yuriko Minegishi , Tatsuhiro Masaoka, Eisuke Iwasaki, Toshifumi Hibi
  • Organizer: Gastrointestinal response to injury.
  • Place of Presentation: Quebec Canada.
  • Year and Date: 2007-10-03
• [Presentation] Sonic hedgehog expression in the gastric mucosa with or without Heterotopic proliferative glands after the H・ pylori eradication. (2007)
  • Author(s): Toshihiro Nishizawa
  • Organizer: Gastroiatestmal response to injury
  • Place of Presentation: Quebec Canada
  • Year and Date: 2007-10-03
• [Presentation] 除菌療法によるsonic hedgehog発現変化と胃粘膜再生 (2007)
  • Author(s): 西澤俊宏, 鈴木秀和,峯岸ゆり子,正岡建洋,岩崎栄典,齋藤義正,日比紀文
  • Organizer: 日本消化器免疫学会総会
  • Place of Presentation: 品川
  • Year and Date: 2007-07-09
• [Presentation] ガチフロキサシンによる三次除菌療法 (2007)
  • Author(s): 西澤俊宏, 鈴木秀和, 正岡建洋, 村岡宏江, 岩崎栄典, 鈴木雅之,小林寅哲, 日比紀文
  • Organizer: 第13 回ヘリコバクター学会
  • Place of Presentation: 大津
  • Year and Date: 2007-06-22
• [Presentation] ガチフロキサシンによる三次除菌療法 (2007)
  • Author(s): 西澤 俊宏
  • Organizer: 第13回ヘリコバクター学会
  • Place of Presentation: 大津
  • Year and Date: 2007-06-22
• [Presentation] Eradication resistance index as a predictor of response to metronidazole-containing second-line treatment for Helicobacter pylori infection (2007)
  • Author(s): Nishizawa, T., Hidekazu Suzuki, Tatsuhiro Masaoka , Eisuke Iwasaki, Toshifumi Hibi
  • Organizer: Digestive Disease Week 2007
  • Place of Presentation: Washington, D. C.
  • Year and Date: 2007-05-23
• [Presentation] Eradication resistance index as a predictor of response to MNZ-containing second-line treatment for H.pylori infection (2007)
  • Author(s): Toshihiro Nishizawa
  • Organizer: Digestive Disease Week 2007
  • Place of Presentation: Washington, D.C
  • Year and Date: 2007-05-23
• [Presentation] Third line H. pylori eradication therapy after the failure of metronidazole-containing second line therapy (2007)
  • Author(s): Nishizawa, T., Suzuki, H., Umezawa, A., Iwasaki, E., Muraoka, H., Masaoka, T., Kobayashi, I., Hibi, T
  • Organizer: The 12th Taishotoyama International Symposium
  • Place of Presentation: Shimoda
  • Year and Date: 2007-04-27
• [Presentation] Third line H. pylori eradication therapy after the failure ofmetronidazole-containing second line therapy. (2007)
  • Author(s): Toshihiro Nishizawa
  • Organizer: Taishotoyama International Symposum.
  • Place of Presentation: Shimoda.
  • Year and Date: 2007-04-27
• [Book] Helicobacter pylori感染症『Annual review2007消化器』林紀夫、日比紀文編集。 (2007)
  • Author(s): 鈴木秀和、西澤俊宏、日比紀文
  • Publisher: 中外医学社。東京。
• [Book] Helicobacter plyor感染症『Annual review 2007 消化器』 (2007)
  • Author(s): 鈴木秀和、西澤俊宏
  • Publisher: 中外医学社