Rapid Detection of Point Mutations Conferring Resistance to Fluoroquinolone in the gyrA of Helicobacter pylori
| Project/Area Number |
19790327
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2007 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥3,030,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | ヘリコバクター・ピロリ / フルオロキノン / gyrA遺伝子 / H.phlore / フルオロキノロン / H.pylori |
|---|
| Research Abstract |
内視鏡後のH. pylori培養検体においてアレル特異的PCR法によりキノロン耐性にかかわるgyrA遺伝子変異株と野生株を2本のPCRチューブで3〜4時間で簡便に識別可能であった。糞便検体については他の腸内細菌のgyrA遺伝子も増幅されてしまい判定不能であった。従来のH. pylori薬剤感受性試験は費用がかかり、10〜14日間の日数を要するが、H. pyloriのキノロン耐性に関わるgyrA遺伝子変異においてアレル特異的PCR法による迅速検出法は簡便で有用と考えられた。
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Report
(3 results)
Research Products
(56 results)
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[Presentation] Prediction of the results of metronidazole-based second-line and gatifloxacin-based third-line therapies after the failure of H. pylori eradication.2007
Author(s)
Nishizawa, T., Suzuki, H., Nakagawa I., Iwasaki, E., Masaoka, T., Hibi, T.
Organizer
The 1st Japan and US Collaboration Conference in Gastroenterology.
Place of Presentation
Tokyo
Year and Date
2007-11-16
Related Report
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[Presentation] Third line H. pylori eradication therapy after the failure of metronidazole-containing second line therapy2007
Author(s)
Nishizawa, T., Suzuki, H., Umezawa, A., Iwasaki, E., Muraoka, H., Masaoka, T., Kobayashi, I., Hibi, T
Organizer
The 12th Taishotoyama International Symposium
Place of Presentation
Shimoda
Year and Date
2007-04-27
Related Report
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