Regulation of virulence gene coordinately expressed with the type 3 protein secretion system in enterohemorrhagic Escherichia coli

• Project/Area Number: 19790330
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: IYODA Sunao National Institute of Infectious Diseases, 細菌第一部, 主任研究官 (70300928)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,720,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2007: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: 腸管出血性大腸菌 / 遺伝子発現制御 / 3型蛋白質輸送装置 / LEE

Research Abstract

腸管出血性大腸菌が保有する病原性遺伝子群LEEの転写発現は、LEEにコードされるLerとGrlAによって正に制御されている。GrlAはLEEの発現活性化因子である一方、べん毛遺伝子群の転写発現を負に制御し、エンテロヘモリシンの転写発現は正に制御する因子であることが明らかとなった。GrlAはLEEと他の病原性遺伝子群の発現を協調的に制御するグローバルレギュレータとして機能していることが示唆された。

Research Products

• [Journal Article] Changing Prevalence of O-serogroups and antimicrobial susceptibility among STEC strains isolated from healthy dairy cows over a decade in Japan between 1998 and 2007. (2009)
  • Author(s): Kobayashi H, Kanazaki M, Ogawa T, Iyoda S, Hara-Kudo Y.
  • Journal Title: J Vet Med Sci 71 Pages: 363-366
  • NAID: 110007131172
  • Peer Reviewed
• [Journal Article] Changing Prevalence of Oserogroups and antimicrobial susceptibility among STEC strains isolated from healthy dairy cows over a decade in Japan between 1998 and 2007. (2009)
  • Author(s): Kobayashi H., et al.
  • Journal Title: J Vet Med Sci 71 Pages: 363-366
  • Peer Reviewed
• [Journal Article] Molecular characterization of enterohemorrhagic Escherichia coli O157:H7 isolates dispersed across Japan by pulsed-field gel electrophoresis and multiple-locus variable-number tandem repeat analysis. (2008)
  • Author(s): Pei Y, Terajima J, Saito Y, Suzuki R, Takai N, Izumiya H, Morita-Ishihara T, Ohnishi M, Miura M, Iyoda S, Mitobe J, Wang B, Watanabe H.
  • Journal Title: Jpn J Infect Dis 61 Pages: 58-64
• [Journal Article] Transcription of the ehx enterohemolysin gene is positively regulated by GrlA, a global regulator encoded within the locus of enterocyte effacement in enterohemorrhagic Escherichia coli. (2008)
  • Author(s): Saitoh T, Iyoda S, Yamamoto S, Lu Y, Shimuta K, Ohnishi M, Terajima J, Watanabe H.
  • Journal Title: J Bacteriol 190 Pages: 4822-4830
  • Peer Reviewed
• [Journal Article] Complete genome sequence and comparative analysis of the wild-type commensal Escherichia coli strain SE11 isolated from a healthy adult. (2008)
  • Author(s): Oshima K, Toh H, Ogura Y, Sasamoto H, Morita H, Park SH, Ooka T, Iyoda S, Taylor TD, Hayashi T, Itoh K, Hattori M.
  • Journal Title: DNA Res 15 Pages: 375-386
  • Peer Reviewed
• [Journal Article] 渡辺治雄腸管出血性大腸菌感染症の現状と細菌学的特徴 (2008)
  • Author(s): 伊豫田淳、寺嶋淳
  • Journal Title: 化学療法の領域 24 Pages: 736-740
• [Journal Article] 腸管出血性大腸菌における病原性遺伝子の協調的発現制御機構 (2008)
  • Author(s): 伊豫田淳
  • Journal Title: 日本細菌学雑誌 63 Pages: 407-415
  • NAID: 10025863620
• [Journal Article] Transcription of the ehx enterohemolysin gene is positively regulated by GrlA, a global regulator encoded within the locus of enterocyte effacement in enterohemorrhagic Escherichia coli. (2008)
  • Author(s): Saitoh T., et al.
  • Journal Title: J Bacteriol. 190 Pages: 4822-4830
  • Peer Reviewed
• [Journal Article] Complete genome sequence and comparative analysis of the wild-type commensal Escherichia coli strain SE11 isolated from a healthy adult. (2008)
  • Author(s): Oshima K., et al.
  • Journal Title: DNA Res. 15 Pages: 375-386
  • Peer Reviewed
• [Journal Article] Molecular characterization of enterohemorrhagic Escherichia coli157:H7 isolates dispersed across Japan by pulsed-field gel electrophoresis and multiplelocus variable-number tandem repeat analysis (2008)
  • Author(s): Pei Y., et. al.
  • Journal Title: Jpn J Infect Dis 61 Pages: 58-64
  • Peer Reviewed
• [Journal Article] Identification of enterohemorrhagic Escherichia coli O157-Specific DNA sequence obtained from amplified fragment length polymorphism analysis. (2007)
  • Author(s): Tokunaga A, Kawano M, Okura M, Iyoda S, Watanabe H, Osawa R.
  • Journal Title: Microbiol Immunol 51 Pages: 883-888
  • NAID: 10020279323
  • Peer Reviewed
• [Journal Article] Identification of enterohemorrhagic Escherichia coli O157-Specific DNA sequence obtained from amplified fragment length polymorphism analysis (2007)
  • Author(s): Tokunaga A., et. al.
  • Journal Title: Microbiol Immunol 51 Pages: 883-888
  • Peer Reviewed
• [Presentation] 腸管出血性大腸菌におけるLEEとエンテロヘモリシンの多重発現制御機構 (2010)
  • Author(s): 伊豫田淳
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋市・名古屋国際会議場
  • Year and Date: 2010-03-12
• [Presentation] 腸管出血性大腸菌における病原性遺伝子群の統括的発現制御因子GrlAの機能に関する遺伝学的解析 (2009)
  • Author(s): 伊豫田淳
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 京都・国立京都国際会館
  • Year and Date: 2009-03-26
• [Presentation] 腸管出血性大腸菌における病原性遺伝子群の統括的発現制御因子GrlAの機能に関する遺伝学的解析 (2008)
  • Author(s): 伊豫 田淳
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 京都・国立京都国際会館
  • Year and Date: 2008-03-26