Study on the mechanism of hepatitis C virus infection-induced cytopathic effect

• Project/Area Number: 19790339
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Kobe University
• Principal Investigator: DENG Lin Kobe University, 大学院・医学研究科, グローバルCOE研究員 (40437497)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,570,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥2,400,000 (Direct Cost: ¥2,400,000)
• Keywords: C型肝炎ウイルス / アポトーシス / ミトコンドリア

Research Abstract

本研究では、HCV clone J6/JFH1株とHuh7.5細胞を用いて、HCV感染初期における肝細胞の細胞障害の分子機序について検討した。HCV感染により、ミトコンドリアにおける活性酸素種(ROS)の産生が増加し、アポトーシス促進タンパク質であるBaxの活性化及びBaxのミトコンドリアへの移行が亢進し、ミトコンドリア障害を介するcaspase-3依存的なアポトーシスが誘導されることを明らかにした。

Research Products

• [Journal Article] Efficient production of infectious hepatitis C virus with adaptive mutations in cultured hepatoma cells (2009)
  • Author(s): Bungyoku Y, Shoji I, Makine T, Adachi T, Hayashida K, Nagano-Fujii M, Ide Y, Deng L, Hotta H.
  • Journal Title: J. Gen. Virol. (in press)
  • Peer Reviewed
• [Journal Article] HCV replication suppresses cellular glucose uptake through down-regulation of cell surface expression of glucose transporters (2009)
  • Author(s): Kasai D, Adachi T, Deng L, Nagano-Fujii M, Sada K, Ikeda M, Kato N, Ide YH, Shoji I, Hotta H.
  • Journal Title: J. Hepatol. 50(5) Pages: 883-894
  • Peer Reviewed
• [Journal Article] HCV replication suppresses cellular glucose uptake through down-regulation of cell surface expression of glucose transporters (2009)
  • Author(s): Daisuke Kasai
  • Journal Title: Journal of Hepatology (In press)
  • Peer Reviewed
• [Journal Article] Efficient production of infectious hepatitis C virus with adaptive mutations in cultured hepatoma cells (2009)
  • Author(s): Yasuaki Bungyoku
  • Journal Title: Journal of General Virology (In press)
  • Peer Reviewed
• [Journal Article] Hepatitis C virus infection induces apoptosis through a Bax-triggered, mitochondrion-mediated, caspase-3-dependent pathway (2008)
  • Author(s): Deng L, Adachi T, Kitayama K, Bungyoku Y, Kitazawa S, Ishido S, Shoji I, Hotta H.
  • Journal Title: J. Virol. 82(21) Pages: 10375-10385
  • Peer Reviewed
• [Journal Article] Hepatitis C virus infection induces apoptosis through a Bax-triggered, mitochondrion-mediated. caspase-3-dependent pathway (2008)
  • Author(s): Lin Deng
  • Journal Title: Journal of Virology 82 Pages: 10375-10385
  • Peer Reviewed
• [Presentation] C型肝炎ウイルス感染によるアポトーシスの分子機序の解析 (2008)
  • Author(s): Deng Lin, 足達哲也, 北山喜九美, 分玉泰彰, 北澤荘平, 石戸総, 勝二郁夫, 堀田博
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2008-10-26
• [Presentation] HCV infection induces Bax-triggered, mitochondria-mediated, caspase-3-dependent apoptosis (2008)
  • Author(s): Deng L, Adachi T, Kitayama K, Bungyoku Y, Kitazawa S, Ishido S, Shoji I, Hotta H.
  • Organizer: 15^<th> international symposium on hepatitis c virus & related virus
  • Place of Presentation: San Antonio, USA
  • Year and Date: 2008-10-07
• [Presentation] Hepatitis C virus infection induces apoptosis via a mitochondrial-related caspase pathway (2007)
  • Author(s): DENG LIN
  • Organizer: I4^th international symposium on hepatitis c viras & related virus
  • Place of Presentation: Glasgow, United Kingdom
  • Year and Date: 2007-11-09
• [Presentation] C型肝炎ウイルス感染による細胞変性効果の分子機序の解析 (2007)
  • Author(s): Deng Lin, 長野基子, 北山喜九美, 安春英, 分玉泰彰, 堀田博
  • Organizer: 第55回日本ウイルス学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2007-10-22
• [Presentation] Hepatitis C virus infection induces apoptosis via a mitochondrial-related caspase pathway (2007)
  • Author(s): Deng L, Nagano-Fujii M, Kitayama K, An C, Bungyoku Y, Hotta H.
  • Organizer: 14^<th> international symposium on hepatitis c virus & related virus
  • Place of Presentation: Glasgow, United Kingdom
  • Year and Date: 2007-09-11