Elucidation of unique cooperative action of multiple viral proteins for efficient virus budding

• Project/Area Number: 19790341
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Hiroshima University
• Principal Investigator: IRIE Takashi Hiroshima University, 大学院・医歯(薬)学総合研究科, 助教 (70419498)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,870,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥570,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2007: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: 出芽 / 粒子形成 / パラミクソウイルス / 出芽・粒子形成 / ウイルスRNA合成 / 自然免疫 / 感染症

Research Abstract

本研究では、センダイウイルス(SeV)をモデルとして、マトリクス蛋白質が原動力となるエンベロープウイルスの粒子形成、出芽を、ウイルス増殖に必須ではないアクセサリー蛋白質であるC蛋白質が促進するメカニズムを解明した。また、研究の過程で、C蛋白質がウイルスの(-)鎖ゲノムRNAと(+)鎖アンチゲノムRNAの合成を制御し、(-)鎖ゲノムRNAをもった感染性ウイルス粒子の産生効率を最適化していることを明らかにした。

Research Products

• [Journal Article] Paramyxovirus Sendai virus C proteins are essential for maintenance of negative-sense RNA genome in virus particles (2008)
  • Author(s): Irie, T., N. Nagata, T. Yoshida, T. Sakaguchi
  • Journal Title: Virology 374 Pages: 495-505
  • Peer Reviewed
• [Journal Article] Recruitment of Alix/AIP1 to the plasma membrane by the Sendai virus C protein facilitates budding of virus-like particles (2008)
  • Author(s): Iire, T., N. Nagata, T. Yoshida, T. Sakaguchi
  • Journal Title: Virology 371 Pages: 108-120
  • Peer Reviewed
• [Journal Article] Contribution of the leader sequence to homologous viral interference among Sendai virus strains (2008)
  • Author(s): Shimazu, Y., S. Takao, T. Irie, K. Kiyotani, T. Yoshida, T. Sakaguchi
  • Journal Title: Virology 372 Pages: 64-71
  • Peer Reviewed
• [Journal Article] Recruitment of Alix/AIPI to the plasma membrane by Sendai virus C protein facilitates budding of virus-like particles (2008)
  • Author(s): 入江 崇
  • Journal Title: Virology 371 Pages: 108-120
  • Peer Reviewed
• [Journal Article] Paramyxovirus Sendai virus C proteins are essential for maintenance of negative-sense RNA genome in virus particles (2008)
  • Author(s): 入江 崇
  • Journal Title: Virology (掲載決定)
  • Peer Reviewed
• [Journal Article] Contribution of the leader sequence to homologous viral interference among Sendai virus strains (2008)
  • Author(s): 島津 幸枝
  • Journal Title: Virology 371 Pages: 64-71
  • Peer Reviewed
• [Journal Article] Modification of the PSAP region of M protein lead to attenuation of VSV in vitro and in vivo (2007)
  • Author(s): Irie, T., E. Carnero, A. Okumura, A. Garcia-Sastre, R.N. Harty
  • Journal Title: J Gen Virol 88 Pages: 2559-2567
  • Peer Reviewed
• [Journal Article] The YLDL sequence within Sendai virus M protein is critical for budding of virus-like particles and interacts with ALix/AIP1 independently of C protein (2007)
  • Author(s): Irie, T., Y. Shimazu, T. Yoshida, T. Sakaguchi
  • Journal Title: J Virol 81 Pages: 12263-73
  • NAID: 120000875252
  • Peer Reviewed
• [Journal Article] Modification of the PSAP region of the matrix protein lead to attenuation of vesicular stomatitis virus in vitro and in vivo (2007)
  • Author(s): 入江 崇
  • Journal Title: Journal of General Virology 88 Pages: 2559-2567
  • Peer Reviewed
• [Journal Article] パラミクソウイルスの出芽機構 (2007)
  • Author(s): 入江 崇
  • Journal Title: ウイルス 57 Pages: 1-8
  • NAID: 10019530623
• [Presentation] センダイウイルスC蛋白質の支援免疫応答に対する機能的多様性 (2008)
  • Author(s): 入江崇
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山県岡山市
  • Year and Date: 2008-10-26
• [Presentation] センダイウイルスM蛋白質YLDL配列の重要性 (2008)
  • Author(s): 坂口剛正(入江崇)
  • Organizer: 第56回日 本ウイルス学会学術集会
  • Place of Presentation: 岡山県岡山市
  • Year and Date: 2008-10-26
• [Presentation] センダイウイルスM蛋白質YLDL配列の重要性 (2008)
  • Author(s): 入江崇
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山県岡山市
  • Year and Date: 2008-10-26
• [Presentation] センダイウイルスC蛋白質の自然免疫応答に対する機能的多様性 (2008)
  • Author(s): 入江崇
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山県岡山市
  • Year and Date: 2008-10-26
• [Presentation] Paramyxovirus Sendai Virus C proteins are essential for maintenance of negative-sense RNA genome in virus particles (2008)
  • Author(s): 入江崇
  • Organizer: The 8th Awaji International Forum in Infection and Immunity
  • Place of Presentation: 兵庫県淡路市
  • Year and Date: 2008-09-10
• [Presentation] Paramyxovirus Sendai Virus C proteins are essential for maintenance of negative-sense RNA genome in virus particles (2008)
  • Author(s): 入江崇
  • Organizer: The 14th International Congress of Virology
  • Place of Presentation: トルコ, イスタンブール
  • Year and Date: 2008-08-11
• [Presentation] センダイウイルスの複製におけるC蛋白質の役割の再考 (2007)
  • Author(s): 入江崇
  • Organizer: 第55回日本ウイルス学会学術集会
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2007-10-22
• [Presentation] Recruitment of Alix/AIP1 to the plasma membrane by Sendai virus C protein facilitates budding of virus-like particles (2007)
  • Author(s): 入江崇
  • Organizer: The 7th Awaji International Forum in Infection and Immunity
  • Place of Presentation: 兵庫県淡路市
  • Year and Date: 2007-09-03
• [Presentation] Recruitment of Alix/AIPl to the plasma membrane by Sendai virus C protein facilitates budding of virus-like particles (2007)
  • Author(s): 入江 崇
  • Organizer: The 7th Awaji International Forum in Infection and Immunity
  • Place of Presentation: 兵庫県淡路市
  • Year and Date: 2007-09-03
• [Presentation] C蛋白質によるセンダイウイルスVLPの出芽促進機構の解明 (2007)
  • Author(s): 入江崇
  • Organizer: 第23回中 国四国ウイルス研究会
  • Place of Presentation: 愛媛県松山市
  • Year and Date: 2007-06-16
• [Presentation] C蛋白質によるセンダイウイルスVLPの出芽促進機構の解明 (2007)
  • Author(s): 入江 崇
  • Organizer: 第23回中国四国ウイルス研究会
  • Place of Presentation: 愛媛県松山市
  • Year and Date: 2007-06-16
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/isaikin/