The serum proteome analysis for discovery of novel biomarkers of hepatocellular carcinoma.

• Project/Area Number: 19790393
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: Chiba University
• Principal Investigator: SOGAWA Kazuyuki Chiba University, 医学部附属病院, 助教 (50436440)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,690,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: プロテオーム / 原発性肝細胞癌 / MALDI-TOF MS / 二次元電気泳動 / Proteimics / Proteomics

Research Abstract

原発性肝細胞癌診断に用いる腫瘍マーカーは画像診断に及ばなく、新たなマーカーの探索が急務である。ペプチド解析では、原発性肝細胞癌患者術前後血清8組(計16検体)を使用し、ClinProt^<TM> systemで解析を行った。Pre-platelet basic proteinの分解産物は術前で著しく発現増大した。タンパク質解析では、同血清を使用し、血清中のメジャータンパク質12種類を除去し、逆相HPLCで分画した後、各フラクションを二次元電気泳動で解析した。apolipoprotein Jは術前で著しく発現増大した。

Research Products

• [Journal Article] A Search for Novel Markers of Alcohol Abuse Using Magnetic beads and MALDI-TOF/TOF Mass Spectrometry. (2009)
  • Author(s): Kazuyuki Sogawa, Mamoru Satoh, Yoshio Kodera, Takeshi Tomonaga, Masaomi Iyo, Fumio Nomura.
  • Journal Title: Proteomics-Clinical applications. (In press.)
  • Peer Reviewed
• [Journal Article] Application of proteomic technologies to discover and identify biomarkers for excessive alcohol consumption. (2007)
  • Author(s): Nomura F, Tomonaga T, Sogawa K, Wu D, Ohashi T.
  • Journal Title: J Chromatogr B Analyt Technol Biomed Life Sci. 855 Pages: 35-41
  • Peer Reviewed
• [Journal Article] Diagnostic values of surface-enhanced laser desorption/ionization technology for screening of habitual drinkers. (2007)
  • Author(s): Sogawa K, Itoga S, Tomonaga T, Nomura F.
  • Journal Title: Alcohol Clin Exp Res. 31
  • Peer Reviewed
• [Journal Article] Diagnostic values of surface-enhanced laser desorption/ionization technology for screening of habitual drinkers. (2007)
  • Author(s): Sogawa K, Itoga S, Tomonaga T, Nomura F.
  • Journal Title: Alcoholism C1nical and Experimental Research Supplement1 Pages: 22-26
  • Peer Reviewed
• [Presentation] Search for Novel Markers of Alcohol Abuse Using three-step proteome analyses. (2009)
  • Author(s): Kazuyuki Sogawa, Mamoru Sato, Yoshio Kodera, Takeshi Tomonaga, Fumio Nomura.
  • Organizer: PepTalk 2009
  • Place of Presentation: San Diego, USA.
  • Year and Date: 2009-01-16
• [Presentation] Search for Novel Markers of Alcohol Abuse Using three-step proteome analyses (2009)
  • Author(s): Sogawa K, Sato M, Kodera Y, Tomonaza T, Nomura F
  • Organizer: PepTalk 2009
  • Place of Presentation: San Diego, California, USA
• [Presentation] ProtemChip system・CmProt systemによる新たな飲酒マーカーの探索・同定 (2008)
  • Author(s): 曽川一幸, 佐藤守, 小寺義男, 朝長毅, 野村, 文夫
  • Organizer: 第57回日本医学検査学会
  • Place of Presentation: 札幌
• [Presentation] プロテオーム解析による新規飲酒マーカーの探索 (2008)
  • Author(s): 曽川一幸、石井知里、佐藤守、小寺義男、朝長毅、野村文夫
  • Organizer: 第6回日本ヒトプロテオーム機構大会
  • Place of Presentation: 大阪
• [Presentation] プロテオーム解析による新規飲酒マーカーの探索・同定 (2008)
  • Author(s): 曽川一幸, 佐藤守, 小寺義男, 朝長毅, 野村文夫
  • Organizer: 日本臨床検査自動化学会第40回大会
  • Place of Presentation: 横浜
• [Presentation] Search for Novel Markers of Alcohol Abuse Using MALDI-TOF MS (2008)
  • Author(s): Sogawa K, Satoh M, Kodera Y, Tomonaga T, Nomura F.
  • Organizer: PepTalk The Definitive Protein-Focused Event
  • Place of Presentation: San Diego, California, USA
• [Presentation] 原発性肝細胞癌の新たなマーカー探索へのthree-step血清プロテオーム解析の応用 (2007)
  • Author(s): 木村明佐子, 曽川一幸, 佐藤守, 小寺義男, 朝長毅, 横須賀收, 野村文夫
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-10-04
• [Presentation] New Strategies for Target, Marker, and Drug Identification Discover a new biomarkerfor early detection of hepatocellular carcinoma using MALDI-TOF MS and ClinProTools. (2007)
  • Author(s): 劉洋, 須永雅彦, 曽川一幸, 佐藤守, 西村基, 根津雅彦, 小寺義男, 朝長毅, 野村文夫
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-10-03
• [Remarks]
  • URL: http://www.m.chiba-u.ac.jp/class/moldiag/
• [Remarks]
  • URL: http://www.m.chiba-u.ac.jp/class/moldiag/index.html
• [Remarks]
  • URL: http://www.m.chiba-u.ac.jp/class/moldiag/index.html